Application of microbial biocementation to improve the physico-mechanical properties of cement mortar

Abo-El-Enein, S.A.; Ali, Ahmad Hadi; Talkhan, Fatma N.; Abdel‐Gawwad, Hamdy A.

doi:10.1016/j.hbrcj.2012.10.004

Cited by 132 publications

(53 citation statements)

References 8 publications

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“…Microbiologically induced mineral precipitation improves the strength by about 25% (Ghosh et al 2005) to 33% (Abo-El-Enein et al 2013) according to the reported literature. The reasons for strength improvement as reported (Ghosh et al 2009) are due to a) the growth of filler material within the pores of the cement mortar b) the formation of new phases of silicates and c) uniform distribution of silicate phases and increased Ca/Si ratio within the C-S-H gel of the matrices due to bacterial treatment.…”

Section: Introductionsupporting

confidence: 61%

Investigation of Bacterial Activity on Compressive Strength of Cement Mortar in Different Curing Media

Thiyagarajan

Maheswaran

Mapa

et al. 2016

ACT

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Recently, bacterial concrete is gaining popularity due to the advantages such as self healing property, increased durability, enhanced strength etc. When living organisms are introduced into cementitious materials, many factors are expected to influence their activity. A conducive environment such as temperature, nutrients, pH etc. is necessary for the survival and intended activity of bacteria in concrete. Among all the influential factors, nutrients for the survival of bacteria in cement mortar is considered in this study. In order to supply nutrients for the bacterial growth, three different curing media such as normal water, Luria Bertania broth (controlled nutrients) and wastewater (uncontrolled nutrients) are chosen. Compressive strength are determined at various stages of curing to study the influence of bacterial activity in cement mortar. Further, X ray diffraction and thermo gravimetric analysis are carried out to understand the transformation in hydration phases with the incorporation of bacteria. From the strength study conducted, cubes cured in waste water showed more strength which indicates that the bacterial activity is more in waste water.

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Section: Introductionsupporting

confidence: 61%

Investigation of Bacterial Activity on Compressive Strength of Cement Mortar in Different Curing Media

Thiyagarajan

Maheswaran

Mapa

et al. 2016

ACT

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“…Thus, may have different reactions. In Xu et al [10] and AboEl-Enein [12] studies, calcium source were added into bioconcrete. After which compressive strength were tested and compared.…”

Section: Compressive Strengthmentioning

confidence: 99%

Calcium Lactate addition in Bioconcrete: Effect on Compressive strength and Water penetration

et al. 2016

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Abstract. This paper presents compressive strength and water penetration of bioconcrete with addition of calcium lactate. Bioconcrete has higher engineering concrete properties and durability compared to normal concrete but the natural production of calcium carbonate is limited to the calcium content in cement. Therefore, additional calcium is added as an additional calcium source to study the influence towards compressive strength and water penetration. The bacteria used in this research are Enterococcus faecalis and Bacillus sp. Calcium lactate was added into concrete mix in concentrations of 0.001mol/l, 0.005mol/l and 0.01mol/l of liquid used. The concentration of bacteria added into the mix is by partial replacement of water used in casting, which are 3% for Enterococcus faecalisand 5% for Bacillus sp. Both compressive strength and water penetration test used cubes of 150mm × 150mm × 150mm. The cubes were tested after 28 days. The result of compressive strength for control is 36 MPa while partial replacement of bacteria yields 38.2 MPa for 3% Enterococcus faecalisand 37.0 MPa for 5% Bacillus sp. Calcium lactate with 0.005 mol/L has the best performance with 42.8 MPa for Enterococcus faecalis and 39.6 MPa for Bacillus sp. Whereas for water penetration, the best concentration of calcium lactate which yielded the lowest water penetration is 0.01 mol/l for both Enterococcus faecalis and Bacillus sp which are 8.7 cm and 8 cm respectively. The addition of calcium lactate into bioconcrete is quite promising for improvement of concrete properties and durability.

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“…On the other hand, calcium carbonate (CaCO3) contributes to increase this resistance, which makes the bioprecipitation a positive contribution [31]. It should be noted that the crystals precipitated by bacteria in cementitious materials, calcium carbonate stand out [12,20,[22][23][30][31][32][33][34][35]. But, to confirm that the crystal precipitated by B. subtilis was calcium carbonate more studies need to be conducted, since this research is a preliminary test.…”

Section: Compressive Strengthmentioning

confidence: 93%

“…3 and 4. It can be seen that in all the compositions in which bacteria were added, there was a crystal precipitation from the 15th day on, possibly calcium carbonate (CaCO3) according to literature [20,22,[30][31][32][33][34][35]. Besides that, the same strain was used in another study [23] that performed an analysis in vitro of the bioprecipitation of calcium carbonate by pH change during biofilm formation.…”

Section: Crack Filling Visual Analysismentioning

confidence: 99%

Crack filling in concrete by addition of Bacillus subtilis spores – Preliminary study

Schwantes-Cezario¹,

Peres²,

Fruet³

et al. 2018

DYNA

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This study aimed to analyze the performance of two concrete mixtures with the addition of B. subtilis spores regarding their capacity of filling cracks during the curing time, evaluating whether the addition can influence the compressive strength of the concrete. For so, six concrete mixtures were studied divided in two mix compositions: 1:1:2 and 1:2:3 (cement: sand: gravel) with w/c of 0.33 and 0.45, respectively. For each composition were added concentrations of 0, 0.3x10 8 or 1.2x10 8 of B. subtilis spores/mL. The results showed that all the samples with spores addition presented crystals precipitation, possibly calcium carbonate, that visually filled the cracks. However, more studies of microstructural analysis should be conducted to prove that the cracks were closed. The compressive strength presented satisfactory results, because the addition of 1.2x10 8 spores/mL did not present significant differences in compressive strength at probability level of 5%, when compared to the reference concrete.Keywords: bioconcrete; concrete with addition of spores; Bacillus subtilis; crack filling; cracks.Relleno de fisuras en concreto por la adición de esporas de Bacilos subtilis -Estudio preliminar ResumenEste estudio tiene como objetivo analizar el desempeño de dos composiciones de hormigón con adición de esporas de B. sutilis con relación a su capacidad de relleno de fisuras durante el periodo de cura, evaluando la influencia de la adición en la resistencia a compresión. Para ello han sido estudiadas seis composiciones de hormigón, divididas en dos dosificaciones: 1:1:2 y 1:2:3 (cemento: árido fino: grava) en peso, con relación a/c de 0,33 y 0,45. Para cada una de las mezclas se utilizaran concentraciones de 0, 0.3x10 8 or 1.2x10 8 de esporas de B. sutilis/mL. Los resultados obtenidos han demostrado que todas las muestras con adición de esporas presentaron precipitación de cristales, posiblemente carbonato de calcio, que fue visualizado en las fisuras. Más estudios microestructurales deberán ser realizados para comprobar que de hecho ocurrió el relleno de las fisuras. La resistencia a compresión presentó resultados satisfactorios, pues no hubo diferencias significativas en relación al hormigón de referencia, a nivel de 5% de probabilidad.Palabras clave: biohormigón; hormigón con adición de esporas; Bacilos subtilis; relleno de fisuras; fisuras.

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Application of microbial biocementation to improve the physico-mechanical properties of cement mortar

Cited by 132 publications

References 8 publications

Investigation of Bacterial Activity on Compressive Strength of Cement Mortar in Different Curing Media

Investigation of Bacterial Activity on Compressive Strength of Cement Mortar in Different Curing Media

Calcium Lactate addition in Bioconcrete: Effect on Compressive strength and Water penetration

Crack filling in concrete by addition of Bacillus subtilis spores – Preliminary study

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