Application of luminescent nanocrystals as labels for biological molecules

Riegler, Jürgen; Nann, Thomas

doi:10.1007/s00216-004-2706-y

Cited by 110 publications

(60 citation statements)

References 33 publications

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“…Recently, having intrinsic excellent optical properties, QDs have been tested as luminescent probes for biological processes, especially fluorescent biological labels and imaging, which have shown the potential biological applications of QDs (5)(6)(7)(8). Nowadays, however, highly efficient QDs are often synthesized in organic media, as such synthesis processes allow for advanced shape and composition control (9).…”

Section: Introductionmentioning

confidence: 99%

Study on the interaction between 2‐mercaptoethanol, dimercaprol and CdSe quantum dots

et al. 2008

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The interactions between 2-mercaptoethanol, dimercaprol and CdSe quantum dots (QDs) in organic media have been investigated by spectral methods. The results showed that the fluorescence (FL) emission of CdSe QDs gradually decreased, with a slight red-shift, after adding thiols to CdSe QDs solutions. With the increase of the concentrations of thiols, the resonance light scattering (RLS) signal of CdSe QDs had been strongly enhanced in the wavelength range 300-500 nm, which was confirmed by the formation of larger CdSe QDs particles. The effect of thiols on the FL emission of CdSe QDs could be described by a Stern-Volmer-type equation with the concentration ranges 1.0 x 10(-6)-7.5 x 10(-4) mol/L for 2-mercaptoethanol and 1.0 x 10(-7)-2.5 x 10(-5) mol/L for dimercaprol. The possible mechanism of the interaction was proposed according to the results of UV-vis absorption and micro-Raman spectroscopy. The results indicated that FL quenching was mainly attributable to the exchange of the QDs surface molecules.

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Section: Introductionmentioning

confidence: 99%

Study on the interaction between 2‐mercaptoethanol, dimercaprol and CdSe quantum dots

et al. 2008

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“…The use of bioinorganic conjugates -QDs/protein systems -could overcome this problem and therefore represent an attractive tool as a novel method for use as bioactive fluorescent probes in sensing, imaging, immunoassays, and other diagnostics applications. [19,59,[78][79][80][81][82][83][84][85][86][87] In 1998, two research groups, Alivisatos et al [89] and Nie et al [56], reported the first use of quantum dots nanocrystals to label single molecules during living-cell assays and this topic is now being intensively investigated.…”

Section: Synthesis Of Colloidal Quantum Dotsmentioning

confidence: 99%

Aqueous Near‐Infrared Fluorescent Composites Based on Apoferritin‐Encapsulated PbS Quantum Dots

et al. 2008

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“…[120][121][122][123][124][125][126][127][128] Here we have selected to discuss a few representative publications that exploit unique quantum dot properties. Figure 60, core/shell quantum dots have narrow linewidths (~25nm FWHM) with a large range of tunability dependent upon the size and composition of the core.…”

Section: Overview Of Biological Applications Of Cdse/zns Nanocrystalsmentioning

confidence: 99%

Synthesis, surface studies, composition and structural characterization of CdSe, core/shell and biologically active nanocrystals

Rosenthal

McBride

Pennycook

et al. 2007

Surface Science Reports

211

209

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Nanostructures, with their very large surface to volume ratio and their non-planar geometry, present an important challenge to surface scientists. New issues arise as to surface characterization, quantification and interface formation. This review summarizes the current state of the art in the synthesis, composition, surface and interface control of CdSe nanocrystal systems, one of the most studied and useful nanostructures. KeywordsCadmium; Selenium; Selenide; CdSe; nanocrystal; quantum dot; nanoparticle; surface; synthesis; electron microscopy; Z-STEM; RBS; alloy; core/shell; fluorescence; quantum yield; growth mechanism; expitaxy; spectroscopy 1.0 Introduction BackgroundWe are currently witnessing an explosion of research in nanoscale science, engineering, and biotechnology. As the nano-revolution proceeds, basic research is leading to the development of true nanotechnologies which have the potential to impact fields from health care and opto-electronics to energy and the environment. One particular type of nanostructure, semiconductor nanocrystals (also known as quantum dots), may ultimately be employed in technologies in each of these fields. For example, antibody-conjugated CdSe/ ZnS core-shell nanocrystals have been used to detect respiratory syncytial virus. 1 The high brightness of the quantum dots enables the virus to be detected in a matter of minutes, whereas the previous assay required 4 days. The ramification of this new technology is that anti-viral drugs can be given early in the infection when they are most effective, reducing Publisher's Disclaimer: This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. NIH Public Access Author ManuscriptSurf Sci Rep. Author manuscript; available in PMC 2011 April 6. NIH-PA Author ManuscriptNIH-PA Author Manuscript NIH-PA Author Manuscript hospitalizations and deaths. In the field of opto-electronics CdSe nanocrystals have been incorporated in light emitting diodes2 -6 and it has been demonstrated that they can serve as the gain media in a laser.7 , 8 Nanocrystals are also poised to play a role in solar energy conversion.9 -14 A specific example is a device in which elongated CdSe nanocrystals (nanorods) are mixed with a conducting polymer and sandwiched between aluminum and indium tin oxide electrodes to create a device with a power conversion efficiency of 1.7 %. 12 Nanocrystals may also have a positive impact on the environment, as it has been shown that they can be used to sequester CO 2 . 15 The spark that ignited two decades of intense research in nanocrystal synthesis, properties, and applications was Louis Brus' demonstration that qua...

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Application of luminescent nanocrystals as labels for biological molecules

Cited by 110 publications

References 33 publications

Study on the interaction between 2‐mercaptoethanol, dimercaprol and CdSe quantum dots

Study on the interaction between 2‐mercaptoethanol, dimercaprol and CdSe quantum dots

Aqueous Near‐Infrared Fluorescent Composites Based on Apoferritin‐Encapsulated PbS Quantum Dots

Synthesis, surface studies, composition and structural characterization of CdSe, core/shell and biologically active nanocrystals

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