Application of Liposomes for Construction of Vaccines

Tuřánek, Jaroslav; Mašek, Josef; Raška, Milan; Ledvina, M

doi:10.5772/21328

Cited by 4 publications

(4 citation statements)

References 59 publications

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“…In fact, it proved to be superior to alum or a combination of alum and an individual PRR ligand . The adjuvanticity of MDP was also shown to depend on the mode of administration; thus, MDP evoked a potent response when delivered with lipophilic carrier systems, such as liposomes . Liposomes have long been used as carriers of antigens and adjuvants to enhance the immune response .…”

Section: Resultsmentioning

confidence: 99%

Discovery of Nanomolar Desmuramylpeptide Agonists of the Innate Immune Receptor Nucleotide-Binding Oligomerization Domain-Containing Protein 2 (NOD2) Possessing Immunostimulatory Properties

et al. 2018

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Muramyl dipeptide (MDP), a fragment of bacterial peptidoglycan, has long been known as the smallest fragment possessing adjuvant activity, on the basis of its agonistic action on the nucleotide-binding oligomerization domain-containing protein 2 (NOD2). There is a pressing need for novel adjuvants, and NOD2 agonists provide an untapped source of potential candidates. Here, we report the design, synthesis, and characterization of a series of novel acyl tripeptides. A pivotal structural element for molecular recognition by NOD2 has been identified, culminating in the discovery of compound 9, the most potent desmuramylpeptide NOD2 agonist to date. Compound 9 augmented pro-inflammatory cytokine release from human peripheral blood mononuclear cells in synergy with lipopolysaccharide. Furthermore, it was able to induce ovalbumin-specific IgG titers in a mouse model of adjuvancy. These findings provide deeper insights into the structural requirements of desmuramylpeptides for NOD2-activation and highlight the potential use of NOD2 agonists as adjuvants for vaccines.

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Section: Resultsmentioning

confidence: 99%

Discovery of Nanomolar Desmuramylpeptide Agonists of the Innate Immune Receptor Nucleotide-Binding Oligomerization Domain-Containing Protein 2 (NOD2) Possessing Immunostimulatory Properties

et al. 2018

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“…Beyond drug delivery, these liposomes have found a niche in vaccine administration, where they can encase antigens and adjuvants to heighten immune responses while reducing side effects (Turánek et al, 2012, Lu et al, 2012. For conditions involving enzyme deficiencies, RPE liposomes facilitate enzyme replacement therapies by delivering functional enzymes to the required sites within the body.…”

Section: The Reverse Phase Evaporation (Rpe) Methodsmentioning

confidence: 99%

Novel Methods for Liposome Formulation: Advancements and Innovations

ENARU,

FĂRCAŞ,

STĂNILĂ

et al. 2023

BUASVMCN-FST

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Liposomes are nanoscale spheroidal vesicles, predominantly composed of phospholipids, that offer a hydrophilic core surrounded by a hydrophobic bilayer. Their unique structure endows them with excellent biocompatibility and the ability to house both hydrophilic and lipophilic agents, making them exceptionally suited for targeted delivery applications. This versatility has seen liposomes gain widespread use in various industries, including medicine, pharmaceuticals, and nutraceuticals. This comprehensive review meticulously examines the spectrum of liposome fabrication techniques, providing insight into their respective advantages and limitations. Specifically, we scrutinize methods such as reverse phase evaporation, freeze drying, the Bangham method (also known as the thin film hydration technique), ethanol injection, the emulsion method, and ultrasonication. Each of these methodologies contributes to the array of liposomal forms, influencing their final characteristics and suitability for different applications. The present review categorizes liposomes based on the fabrication technique, offering a comparative perspective that is instrumental for researchers and practitioners in selecting the optimal method for their specific delivery system needs.

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“…Synthesis. 1 H and 13 C NMR spectra were measured on a Bruker AVANCE instruments-500, Bruker AVANCE-400, and Varian UNITY INOVA 400 ( 1 H at 500, 400 MHz, 13 C at 125, and 100 MHz). Chemical shifts (δ) of protons and carbons are given in units of ppm and coupling constants (J) in Hz.…”

Section: ■ Conclusionmentioning

confidence: 99%

“…In particular, the internal aqueous space can be used to encapsulate hydrophilic agents whereas the phospholipid bilayer can be used to anchor lipophilic agents via their hydrophobic domain(s) . The latter approach has been used successfully for the construction of targeted liposomes, lipid-based microbubbles, and liposomal vaccines with bioactive agents mounted on liposome surfaces. ,,,− …”

Section: Introductionmentioning

confidence: 99%

Hyaluronic Acid Surface Modified Liposomes Prepared via Orthogonal Aminoxy Coupling: Synthesis of Nontoxic Aminoxylipids Based on Symmetrically α-Branched Fatty Acids, Preparation of Liposomes by Microfluidic Mixing, and Targeting to Cancer Cells Expressing CD44

et al. 2018

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New synthetic aminoxy lipids are designed and synthesized as building blocks for the formulation of functionalized nanoliposomes by microfluidization using a NanoAssemblr. Orthogonal binding of hyaluronic acid onto the outer surface of functionalized nanoliposomes via aminoxy coupling ( N-oxy ligation) is achieved at hemiacetal function of hyaluronic acid and the structure of hyaluronic acid-liposomes is visualized by transmission electron microscopy and cryotransmission electron microscopy. Observed structures are in a good correlation with data obtained by dynamic light scattering (size and ζ-potential). In vitro experiments on cell lines expressing CD44 receptors demonstrate selective internalization of fluorochrome-labeled hyaluronic acid-liposomes, while cells with down regulated CD44 receptor levels exhibit very low internalization of hyaluronic acid-liposomes. A method based on microfluidization mixing was developed for preparation of monodispersive unilamellar liposomes containing aminoxy lipids and orthogonal binding of hyaluronic acid onto the liposomal surface was demonstrated. These hyaluronic acid-liposomes represent a potentially new drug delivery platform for CD44-targeted anticancer drugs as well as for immunotherapeutics and vaccines.

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Application of Liposomes for Construction of Vaccines

Cited by 4 publications

References 59 publications

Discovery of Nanomolar Desmuramylpeptide Agonists of the Innate Immune Receptor Nucleotide-Binding Oligomerization Domain-Containing Protein 2 (NOD2) Possessing Immunostimulatory Properties

Discovery of Nanomolar Desmuramylpeptide Agonists of the Innate Immune Receptor Nucleotide-Binding Oligomerization Domain-Containing Protein 2 (NOD2) Possessing Immunostimulatory Properties

Novel Methods for Liposome Formulation: Advancements and Innovations

Hyaluronic Acid Surface Modified Liposomes Prepared via Orthogonal Aminoxy Coupling: Synthesis of Nontoxic Aminoxylipids Based on Symmetrically α-Branched Fatty Acids, Preparation of Liposomes by Microfluidic Mixing, and Targeting to Cancer Cells Expressing CD44

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