Application of impedance spectroscopy for monitoring colloid Au-enhanced antibody immobilization and antibody–antigen reactions

Gold Nanoparticles in Biomedical Applications

“…Hepatitis B virus surface antigen was detected using electrochemical impedance spectroscopy (EIS) through immobilization of the antibody onto gold nanoparticles-modified 4-aminothiophenol self-assembled monolayer's. 51 Potentiometric and amperometric immunosensors for Hepatitis B virus surface antigen detection were also constructed by electrostatic adsorption of the antibody onto gold nanoparticles/tris (2, 2-bipyridyl) cobalt (III) multilayer films 52 or by immobilization of the antibody onto gold nanoparticles-modified thiol-containing sol-gel network 53 The antigen-antibody reaction is detected through measuring the changes in the electric potential before and after. Furthermore, a different strategy was used to fabricate an amperometric immunosensor based on immobilization of hepatitis B antibody on a gold electrode modified with gold nanoparticles and horseradish peroxidase 54 in order to avoid non-specific adsorption and also amplify the response of the antigen-antibody reaction.…”

Electrochemical sensor systems based on one dimensional (1D) nanostructures for analysis of bioaffinity interactions

“…Механизм иммобилизации заключается во встраивании антител в матрицу ДДАБ и взаимодействии антител с золотыми наночастицами (возможно за счёт SH-групп). Коллоидные частицы золота имеют большую площадь поверхности и хорошую биосовместимость [26]. Использование ансамбля нанопроводов на электроде позволяет поднять чувствительность системы.…”

Direct Immunosensor Design Based on the Electrochemical Reduction of 4-((4-Nitrophenyl)ethynyl)benzenethiol Monolayers

“…For immunosensor development, this carbohydrate region has become increasingly important and has opened new avenues for Ab surface immobilization strategies [13][14][15][16]. The carbohydrate residues of the Fc region are oxidized with periodate to give aldehydes [17], which can then be reacted with a number of functional groups to covalently bind the Ab to the surface [18][19][20]. Typically, amines, hydrazines, and hydrazides are used to immobilize the oxidized Ab.…”