“…Probably, the acidic nature of humic substances plays an essential role in their reactivity and performance in alkaline soils. The abundance of different functional groups in humic acid, especially acid functional groups like carboxyl, determines their adsorption capacities for HMs (Yang, Miao et al 2015, Bezuglova andKlimenko 2022). In expressing the effect of humic substances in the growth process of plants, the following have been reported: I) improvement in root formation and development of the assimilation apparatus (Canellas, Olivares et al 2002, Zandonadi, Santos et al 2010, Mora, Baigorri et al 2012, changes of phosphate metabolism and energy transfer (Rosa, Silva et al 2018, de Castro, Berbara et al 2021, acceleration in protein metabolism (Vaughan 1974, Vaccaro, Ertani et al 2015, increase in the intensity of respiration processes, photosynthesis, and water exchange and increasing the concentration of chlorophyll and ascorbic acid (Liu, Cooper et al 1998, Lotfi, Kalaji et al 2018, Shamsullah 2020.…”