Application of DNA Typing Methods to the Study of the Epidemiology of Malassezia pachydermatis

Anthony, Richard; Howell, S. A.; Lloyd, D. H.; Pinter, Ljiljana

doi:10.3109/08910609409141346

Cited by 22 publications

(28 citation statements)

References 16 publications

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“…Karyotyping was carried out as described previously (Anthony et al, 1994) with ten randomly chosen isolates. They were harvested after 24 h growth on Sabouraud's dextrose agar.…”

Section: Electrophoretic Karyotypingmentioning

confidence: 99%

“…They were harvested after 24 h growth on Sabouraud's dextrose agar. DNA was extracted by a modification of the method described by Carl and Olson (1985) and gels run as described previously (Anthony et al, 1994).…”

Section: Electrophoretic Karyotypingmentioning

confidence: 99%

“…DNA was extracted as described previously (Anthony et al, 1994). Briefly: cell walls were disrupted by incubating about 0.2 g of the washed cells in 0.5 ml of LET buffer [0.5 M EDTA, 0.01 M Tris and 2% NaOH (pH 7.5)] containing 1 mg of Zymolyase 20T and 1 mg of Novozym, mixing well and holding at 30 °C for 5 h. The spheroplasted cells were centrifuged at 10,000 g and the supernatant discarded then lysed by re-suspending with vigorous mixing in 0.5 ml of GES reagent (5 M guanidium thiocyanate in 100 mM EDTA plus 0.5% N-laurylsarcosine) and holding at room temperature for 20 min.…”

Section: Restriction Fragment Length Polymorphism (Rflp) Analysismentioning

confidence: 99%

“…Twenty µl of the DNA prepared in this way was restricted with BglII to the manufacturers instructions and run on an 0.8% agarose gel at a constant 30 volts for 18 h. For Southern hybridisations DNA was capillary blotted onto Stratagene nylon membranes. Hybridisations were carried out using biotin-labelled poly (GT) (Anthony et al, 1994).…”

Section: Restriction Fragment Length Polymorphism (Rflp) Analysismentioning

confidence: 99%

“…Non-lipid dependent Malassezia yeasts are only occasionally isolated from pig skin (Kuttin and Glas, 1985;Melzig, 1989). It has been demonstrated that all isolates of M. pachydermatis share a remarkably homogeneous chromosome profile by karyotyping and therefore karyotyping provides an effective method of confirming the species of isolates (Anthony et al, 1994). Restriction digestion has been shown to discriminate between different strains of M. pachydermatis isolated from dogs and humans and increased discrimination has been described with the use of a simple repeat poly (GT) probe (Anthony et al, 1994).…”

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confidence: 99%

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Apparent cross-infection with a single strain of Malassezia pachydermatis on a pig farm

Pinter

Anthony

Glumac

2002

Acta Veterinaria Hungarica

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Twenty-nine isolates of Malassezia pachydermatis were recovered from a single farm of 100 pigs in Croatia. In contrast, 290 farm pigs from other locations (northern parts of Croatia and Slovenia) yielded only two non-lipid dependent isolates of M. pachydermatis using the same swabbing procedure. Ten of the 29 isolates from a single farm had their identity confirmed by karyotyping, and were typed by restriction fragment length polymorphism (RFLP) analysis. All but one of these isolates sub-typed were indistinguishable, one isolate produced a slightly different RFLP profile. Control isolates recovered from dog skin gave RFLP profiles that were easily distinguished from those produced by the pig isolates. These results suggest that a single strain of M. pachydermatis had colonised this pig herd.Key words: DNA restriction, Malassezia pachydermatis, pig For quite a long time, members of the genus Malassezia isolated from humans have been classified in two different entities, Pityrosporum ovale and Pityrosporum orbiculare and those isolated from animals have been named Pityrosporum canis. Since 1995 the taxonomy of the genus Malassezia has been revised according to morphology and molecular biological techniques . As a result of this the genus Malassezia has been enlarged to include seven distinct species, three former taxa (M. pachydermatis, M. furfur and M. sympodialis) and four new taxa (M. globosa, M. obtusa, M. restricta and M. slooffiae). All species except M. pachydermatis are lipiddependent (basidiomyceteous) yeasts. Members of this genus belong to the normal mammalian cutaneous microflora and are also believed to act as opportunistic human and animal pathogens (causing seborrhoeic dermatitis, pityriasis versicolor, folliculitis, otitis and some forms of atopic dermatitis).

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“…Karyotyping was carried out as described previously (Anthony et al, 1994) with ten randomly chosen isolates. They were harvested after 24 h growth on Sabouraud's dextrose agar.…”

Section: Electrophoretic Karyotypingmentioning

confidence: 99%

Section: Electrophoretic Karyotypingmentioning

confidence: 99%

Section: Restriction Fragment Length Polymorphism (Rflp) Analysismentioning

confidence: 99%

Section: Restriction Fragment Length Polymorphism (Rflp) Analysismentioning

confidence: 99%

mentioning

confidence: 99%

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Apparent cross-infection with a single strain of Malassezia pachydermatis on a pig farm

Pinter

Anthony

Glumac

2002

Acta Veterinaria Hungarica

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The genus Malassezia with description of four new species

Guého

Midgley

Guillot

1996

Antonie van Leeuwenhoek

563

465

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The genus Malassezia has been revised using morphology, ultrastructure, physiology and molecular biology. As a result the genus has been enlarged to include seven species comprising the three former taxa M. furfur, M. pachydermatis and M. sympodialis, and four new taxa M. globosa, M. obtusa, M. restricta and M. slooffiae. The descriptions of all the species include morphology of the colonies and of the cells, together with ultrastructural details. The physiological properties studied were the presence of catalase, the tolerance of 37 degrees C and the ability to utilize certain concentrations of Tween 20, 40, 60 and 80 as a source of lipid in a simple medium. Information is given for each of the taxa on mole% GC and also the rRNA sequence from the comparison previously described for the genus.

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Cryptic Diversity of Malassezia pachydermatis from Healthy and Diseased Domestic Animals

2016

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Malassezia pachydermatis is part of the normal cutaneous microbiota of wild and domestic carnivores. However, under certain conditions this yeast can overproliferate and cause several diseases in its host, mainly otitis and dermatitis in dogs. The aim of this study was to conduct a molecular characterization of M. pachydermatis isolates from healthy and diseased domestic animals, in order to assess the molecular diversity and phylogenetic relationship within this species. The large subunit (LSU) and the internal transcribed spacer (ITS) of ribosomal RNA, chitin synthase 2 (CHS2) and β-tubulin genes from sixteen strains isolated from dogs, cats, a goat, a pig and a horse were sequenced. A different number of types of sequences were identified for each target gene, including some types described for the first time. Five sequence types were characterized for the LSU, eleven for the ITS region, nine for CHS2 and eight for β-tubulin. A multilocus analysis was performed including the four genes, and the resulting phylogenetic tree revealed fifteen genotypes. Genotypes were distributed in two well-supported clades. One clade comprised strains isolated from different domestic animals and a strongly supported cluster constituted by strains isolated from cats. The second clade included strains isolated mainly from dogs and an outlier strain isolated from a horse. No apparent association could be observed between the health status of the animal hosts and concrete strains. The multilocus phylogenetic analysis is a useful tool to assess the intraspecific variation within this species and could help understand the ecology, epidemiology and speciation process of M. pachydermatis.

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Application of DNA Typing Methods to the Study of the Epidemiology of Malassezia pachydermatis

Cited by 22 publications

References 16 publications

Apparent cross-infection with a single strain of Malassezia pachydermatis on a pig farm

Apparent cross-infection with a single strain of Malassezia pachydermatis on a pig farm

The genus Malassezia with description of four new species

Cryptic Diversity of Malassezia pachydermatis from Healthy and Diseased Domestic Animals

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