Application of chitosan spraying on acclimatization success of tiger orchid (Grammatophyllum scriptum) plantlets

Pitoyo, Ari; Hani, Mutia Rizka; Anggarwulan, Endang

doi:10.13057/nusbiosci/n070222

Cited by 8 publications

(6 citation statements)

References 2 publications

(1 reference statement)

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“…Kananont et al [51] studied the effects of various types of CHT at different concentrations on the seed germination of various orchid species and found that none of the six CHT types tested (CHT polymers: P 70 , P 80 Chitosan is also known to stimulate plantlet growth and development under greenhouse conditions. Pitoyo et al [56] studied the effects of CHT on the growth of Grammatophyllum scriptum plantlets and suggested that CHT had significant effects on some parameters, including plantlet height and leaf length. Kumari et al [57] investigated the effects of different CHT concentrations (2.5, 5, 7.5, and 10 mg•L −1 ) on the growth and development of Dendrobium cv.…”

Section: Chitosanmentioning

confidence: 99%

Organic Compounds: Contents and Their Role in Improving Seed Germination and Protocorm Development in Orchids

Utami

Hariyanto

2020

International Journal of Agronomy

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In nature, orchid seed germination is obligatory following infection by mycorrhizal fungi, which supplies the developing embryo with water, carbohydrates, vitamins, and minerals, causing the seeds to germinate relatively slowly and at a low germination rate. The nonsymbiotic germination of orchid seeds found in 1922 is applicable to in vitro propagation. The success of seed germination in vitro is influenced by supplementation with organic compounds. Here, we review the scientific literature in terms of the contents and role of organic supplements in promoting seed germination, protocorm development, and seedling growth in orchids. We systematically collected information from scientific literature databases including Scopus, Google Scholar, and ProQuest, as well as published books and conference proceedings. Various organic compounds, i.e., coconut water (CW), peptone (P), banana homogenate (BH), potato homogenate (PH), chitosan (CHT), tomato juice (TJ), and yeast extract (YE), can promote seed germination and growth and development of various orchids. They also stimulate seedling development, formation of protocorm-like bodies (PLBs), plantlet growth, and multiple shoot formation. The addition of organic compounds to culture media, individually or in combination, accelerates seed germination and seedling development. Different types and concentrations of organic nutrients are needed for the success of in vitro cultures, depending on the species and genotype.

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Section: Chitosanmentioning

confidence: 99%

Organic Compounds: Contents and Their Role in Improving Seed Germination and Protocorm Development in Orchids

Utami

Hariyanto

2020

International Journal of Agronomy

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“…After meristem culture and micropropagation of some cassava varieties [11], the seedlings were acclimatized without taking into account the graduation conditions in acclimatization process, and other environmental and intrinsic factors in plantlets [12]. In fact, the nutrient watering solutions, relative humidity and temperature constitute important factors that determine the acclimatization process [13] [14]. Moreover, the effect of substrates is very decisive in the acclimatization success [15] [16] but few have discussed the use of local products including palm fibers and sawdust instead of peat and vermiculite.…”

Section: Introductionmentioning

confidence: 99%

Effects of Non-Conventional Substrate and NPK Fertilizer on Cassava Plantlets Acclimatization for Plant Material Production

Cacaï¹,

Ahanhanzo²,

Ahoya³

et al. 2021

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The adaptation of cassava plantlets to natural conditions remains the main obstacle in the process of free virus plantlets production. In this study, cassava plantlets acclimatization was assessed by pre-acclimatization, height of acclimated plantlets, substrate, and nutrient watering solution. The results revealed that pre-acclimatization gave a high survival rate (61.9%) whereas the direct tunnel acclimatization was conducted to low rate (27.31%) of plantlets. Furthermore, the height of plantlets influenced significantly (p < 0.05) their survival rate with best survival rate observed on plantlet height from 1 cm to 5 cm. There was no significant difference between the substrates used and watering solutions. However, the Sawdust substrate gave a high survival rate (46.67%) and better phylogenesis (1.8) in the variety RB 89509. Likewise, plantlets treated with NPK nutrient watering solutions (20-10-10) had a better survival rate (48.57%), better phylogenesis (2.125) with the variety RB 89509 and better growth in height (1.18 cm) with the variety BF92/0267. These findings constitute a database for the establishment of the technical itinerary for the acclimatization of cassava plantlets.

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“…are paracytic; and shaped of guard cells are kidney . The type of stomata of Grammatophyllum scriptum is mostly tetracytic, there are four epidermal cells surrounding by two guard cells (Pitoyo et al 2015). was studied on dermal anatomy of two interesting corticolous orchids from Western Ghats, Cymbidium aloifolium Swart and Luisia birchea (A.…”

Section: Resultsmentioning

confidence: 99%

Stomata cells studies of Paraphalaenopsis spp. from in vitro and greenhouse condition

Garvita

Wawangningrum

2020

Biodiversitas

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Abstract. Garvita RV, Wawangningrum H. 2020. Stomata cells studies of Paraphalaenopsis spp. from in vitro and greenhouse condition. Biodiversitas 21: 1116-1121. In vitro micropropagation is an efficient biotechnological strategy for conservation and commercial plantlet production. The ultimate success of in vitro microporapagation depends on the ability to transfer plants out of culture, known as acclimatization stage. The morphology, anatomy and physiology of plantlets in in vitro culture conditions is different from the plants in environment condition (greenhouse), therefore they are unable to compete with soil microbes and to cope with the environmental conditions. The aim of this research is to determine stomata morphology and distribution of Paraphalaenopsis spp (Orchidaceae) from in vitro growth and greenhouse conditions. The leaf stomata cells of Paraphalaenopsis spp. (Paraphalaenopsis serpentilingua, Paraphalaenopsis labukensis and Paraphalaenopsis laycockii) was undertaken by observing the stomata preparation of the upper leaf and lower leaf by using microscope fitted with optic visual seven. The descriptive method was used to describe the stomata morphology by observing the epidermis of longitudinal section and stomata length of upper and lower leaf by using a microscope. The results showed that these plants had anomocytic type of stomata cells. The distributions of stomata are in the upper and lower epidermis leaf in all Paraphalaenopsis spp from in vitro growth and greenhouse.

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Application of chitosan spraying on acclimatization success of tiger orchid (Grammatophyllum scriptum) plantlets

Cited by 8 publications

References 2 publications

Organic Compounds: Contents and Their Role in Improving Seed Germination and Protocorm Development in Orchids

Organic Compounds: Contents and Their Role in Improving Seed Germination and Protocorm Development in Orchids

Effects of Non-Conventional Substrate and NPK Fertilizer on Cassava Plantlets Acclimatization for Plant Material Production

Stomata cells studies of Paraphalaenopsis spp. from in vitro and greenhouse condition

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