Application of Chitinous Materials in Production and Purification of a Poly(l-lactic acid) Depolymerase from Pseudomonas tamsuii TKU015

Liang, Tzu Wen; Jen, Shan Ni; Nguyen, Anh Dzung; Wang, San-Lang

doi:10.3390/polym8030098

Cited by 21 publications

(15 citation statements)

References 29 publications

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“…Optimum temperature and pH for PLA depolymerase activity are 60 °C and 10, respectively. Liang et al (2016) purified the 58-kDa PLA depolymerase from Pseudomonas tamsuii TKU015. Sangwan and Wu (2008) exploited molecular techniques and reported genera Thermopolyspora, Thermomonospora and Paecilomyces for PLA degradation under controlled (aerobic) composting.…”

Section: Polylactic Acid (Pla)mentioning

confidence: 99%

Review on the current status of polymer degradation: a microbial approach

Pathak

Bithel

2017

Bioresour. Bioprocess.

501

214

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Inertness and the indiscriminate use of synthetic polymers leading to increased land and water pollution are of great concern. Plastic is the most useful synthetic polymer, employed in wide range of applications viz. the packaging industries, agriculture, household practices, etc. Unpredicted use of synthetic polymers is leading towards the accumulation of increased solid waste in the natural environment. This affects the natural system and creates various environmental hazards. Plastics are seen as an environmental threat because they are difficult to degrade. This review describes the occurrence and distribution of microbes that are involved in the degradation of both natural and synthetic polymers. Much interest is generated by the degradation of existing plastics using microorganisms. It seems that biological agents and their metabolic enzymes can be exploited as a potent tool for polymer degradation. Bacterial and fungal species are the most abundant biological agents found in nature and have distinct degradation abilities for natural and synthetic polymers.

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Section: Polylactic Acid (Pla)mentioning

confidence: 99%

Review on the current status of polymer degradation: a microbial approach

Pathak

Bithel

2017

Bioresour. Bioprocess.

501

214

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“…Chitin, chitosan, colloidal chitin, and water soluble chitosan have commonly been used as the major carbon/nitrogen (C/N) sources for the isolation of strains producing chitinolytic enzymes and the production of chitinolytic enzymes by these isolated strains [ 9 , 15 , 16 , 17 , 18 ]. To cut down fermentation expenses, the inexpensive materials of shrimp heads, shrimp shells, crab shells, and squid pens have recently been evaluated as the sole C/N sources for the production of bioactive compounds [ 5 , 6 , 9 , 12 , 13 , 14 , 15 , 16 , 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40 , 41 , 42 , 43 , 44 , 45 , 46 , 47 , 48 , 49 , 50 , 51 , 52 , 53 , 54 , 55 ,…”

Section: Introductionmentioning

confidence: 99%

Production and Potential Applications of Bioconversion of Chitin and Protein-Containing Fishery Byproducts into Prodigiosin: A Review

et al. 2020

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The technology of microbial conversion provides a potential way to exploit compounds of biotechnological potential. The red pigment prodigiosin (PG) and other PG-like pigments from bacteria, majorly from Serratia marcescens, have been reported as bioactive secondary metabolites that can be used in the broad fields of agriculture, fine chemicals, and pharmacy. Increasing PG productivity by investigating the culture conditions especially the inexpensive carbon and nitrogen (C/N) sources has become an important factor for large-scale production. Investigations into the bioactivities and applications of PG and its related compounds have also been given increased attention. To save production cost, chitin and protein-containing fishery byproducts have recently been investigated as the sole C/N source for the production of PG and chitinolytic/proteolytic enzymes. This strategy provides an environmentally-friendly selection using inexpensive C/N sources to produce a high yield of PG together with chitinolytic and proteolytic enzymes by S. marcescens. The review article will provide effective references for production, bioactivity, and application of S. marcescens PG in various fields such as biocontrol agents and potential pharmaceutical drugs.

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“…To reduce costs and recycle chitinous processing materials more efficiently, shrimp shells, crab shells and squid pens have been used as the sole C/N sources for screening chitinolytic and proteolytic enzyme-producing bacteria [ 7 , 8 , 9 , 10 ]. Unlike chitin and chitosan, chitin-containing fishery byproducts show greater potential as C/N sources since they can produce chitinolytic enzymes, proteases and other high-value-added bioactive materials [ 11 , 12 , 13 , 14 , 15 , 16 , 17 , 18 , 19 ].…”

Section: Introductionmentioning

confidence: 99%

“…Among chitin-containing fishery byproducts, squid pens contain the highest percentage of proteins and are most suitable for producing enzymes [ 7 , 8 , 9 , 10 , 11 ] and various bioactive compounds, such as antioxidants [ 13 , 14 , 15 ], α-glucosidase inhibitors (αGI) [ 16 , 17 , 18 ], biofertilizers [ 19 , 20 ], compounds with antitumor properties [ 13 ], biosurfactants [ 21 ], exopolysaccharides [ 21 , 22 , 23 ], insecticidal materials [ 24 , 25 ] and chitin, as well as chitin oligomers [ 20 ].…”

Section: Introductionmentioning

confidence: 99%

Conversion of Squid Pens to Chitosanases and Proteases via Paenibacillus sp. TKU042

et al. 2018

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Chitosanases and proteases have received much attention due to their wide range of applications. Four kinds of chitinous materials, squid pens, shrimp heads, demineralized shrimp shells and demineralized crab shells, were used as the sole carbon and nitrogen (C/N) source to produce chitosanases, proteases and α-glucosidase inhibitors (αGI) by four different strains of Paenibacillus. Chitosanase productivity was highest in the culture supernatants using squid pens as the sole C/N source. The maximum chitosanase activity of fermented squid pens (0.759 U/mL) was compared to that of fermented shrimp heads (0.397 U/mL), demineralized shrimp shells (0.201 U/mL) and demineralized crab shells (0.216 U/mL). A squid pen concentration of 0.5% was suitable for chitosanase, protease and αGI production via Paenibacillus sp. TKU042. Multi-purification, including ethanol precipitation and column chromatography of Macro-Prep High S as well as Macro-Prep DEAE (diethylaminoethyl), led to the isolation of Paenibacillus sp. TKU042 chitosanase and protease with molecular weights of 70 and 35 kDa, respectively. For comparison, 16 chitinolytic bacteria, including strains of Paenibacillus, were investigated for the production of chitinase, exochitinase, chitosanase, protease and αGI using two kinds of chitinous sources.

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Application of Chitinous Materials in Production and Purification of a Poly(l-lactic acid) Depolymerase from Pseudomonas tamsuii TKU015

Cited by 21 publications

References 29 publications

Review on the current status of polymer degradation: a microbial approach

Review on the current status of polymer degradation: a microbial approach

Production and Potential Applications of Bioconversion of Chitin and Protein-Containing Fishery Byproducts into Prodigiosin: A Review

Conversion of Squid Pens to Chitosanases and Proteases via Paenibacillus sp. TKU042

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