Application of cellular biosensors for detection of atypical toxic bioactivity in microcystin-containing cyanobacterial extracts

Mankiewicz-Boczek, Joanna; Karwaciak, Iwona; Ratajewski, Marcin; Gągała, Ilona; Jurczak, Tomasz; Zalewski, Maciej; Pułaski, Łukasz

doi:10.1016/j.aquatox.2015.09.004

Cited by 12 publications

(5 citation statements)

References 46 publications

(62 reference statements)

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“…Further, new cyanotoxins are continuously being discovered and this work should continue to remain at the forefront of protecting human and ecological health. 99,100 For example, Mankiewics-Boczek et al (2015) recently found that cellular biosensors detected toxic bioactivity in cyanobacterial extracts that exceeded toxicity levels for MCs; including effects on lipopolysaccharides and cellular wall components. 100 Finally, the toxicity of degradation products of MCs should be explored.…”

Section: Discussionmentioning

confidence: 99%

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Toxic mechanisms of microcystins in mammals

2017

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Microcystins, such as microcystin-leucine arginine (MC-LR), are some of the most toxic and prevalent cyanotoxins produced by cyanobacteria in freshwater and saltwater algal blooms worldwide. Acute and chronic exposures to microcystins are primarily known to cause hepatotoxicity; cellular damage and genotoxicity within mammalian livers. However, studies indicate that similar damage may occur in other mammalian organs and tissues, such as the kidney, heart, reproductive systems, and lungs - particularly following chronic low-dose exposures. Mechanisms of toxicity of mycrocystins are reviewed herein; including cellular uptake, interaction with protein phosphatases PP1 and PP2A, cytoskeletal effects, formation of oxidative stress and induction of apoptosis. In general, the mode of action of toxicity by MCs in mammalian organs are similar to those that have been observed in liver tissues. A comprehensive understanding of the toxic mechanisms of microcystins in mammalian tissues and organs will assist in the development of risk assessment approaches to public health protection strategies and the development of robust drinking water policies.

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Section: Discussionmentioning

confidence: 99%

“…99,100 For example, Mankiewics-Boczek et al (2015) recently found that cellular biosensors detected toxic bioactivity in cyanobacterial extracts that exceeded toxicity levels for MCs; including effects on lipopolysaccharides and cellular wall components. 100 Finally, the toxicity of degradation products of MCs should be explored. 101,102…”

Section: Discussionmentioning

confidence: 99%

Toxic mechanisms of microcystins in mammals

2017

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“…Commercially available adenocarcinoma cell lines were used for the assessment of the effect of the compounds on the activity of transcription factor AP-1 (Luciferase Reporter Stable HeLa cell line, 350-SL-0019-NP; Signosis, Inc., Santa Clara, CA, USA). A stable ovarian cancer reporter cell line NF-κB (SkoV3-Nf-κB) from the Regulation Laboratory IBM PAN, Poland, derived as previously described [68,69], was used for the assessment of NF-κB promoter activity. The cells were seeded into 96-well plates in the density of 1 × 10 4 cell/well and maintained in culture medium for 24 h to allow attachment to the plate surface.…”

Section: Reporter Gene Cell Lines and Ap-1 And Nf-κb Activity Assaysmentioning

confidence: 99%

Interaction of Adenosine, Modified Using Carborane Clusters, with Ovarian Cancer Cells: A New Anticancer Approach against Chemoresistance

Bednarska-Szczepaniak

Przelazły

Kania

et al. 2021

Cancers

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Platinum compounds remain the first-line drugs for the treatment of most lethal gynecological malignancies and ovarian cancers. Acquired platinum resistance remains a major challenge in gynecological oncology. Considering the unique physicochemical properties of the metallacarboranes modifier and the significant role of nucleoside derivatives as anticancer antimetabolites, we designed and synthesized a set of adenosine conjugates with metallacarboranes containing iron, cobalt, or chromium as semi-abiotic compounds that influence the cisplatin sensitivity of ovarian cancer cells. Adherent cultures of ovarian carcinoma cell lines and multicellular spheroids, ranging from sensitive to highly resistant including experimental cell lines “not responding” to platinum drugs were used. Iron-containing metallacarborane conjugates showed the best anticancer activity, especially against resistant cells. Compound modified at the C2′ nucleoside position showed the best activity in resistant cancer cells and highly resistant cancer spheroids exposed to cisplatin, increasing cell cycle arrest, apoptosis or necrosis, and reactive oxygen species production. Moreover, it showed high cellular accumulation and did not induce cross-resistance to cisplatin, carboplatin, doxorubicin, paclitaxel, or gemcitabine in long-term cultures. The reference nido-carborane derivative (no metal ions) and unmodified nucleosides were not as effective. These findings indicate that metallacarborane modification of adenosine may sensitize ovarian cancer cells to cisplatin in combination treatment.

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“…Microcystins, mainly produced by freshwater blue-green algae, are the most common algal toxins. They have the highest occurrence frequency in cyanobacteria blooms and have already resulted in very serious impacts on people [9,10]. Among microcystins, microcystin-LR (MC-LR) has been shown to be one of the most toxic and harmful algal toxins [11].…”

Section: Removal Of Chlorophyll-a and Microcystin-lrmentioning

confidence: 99%

Removal Characteristics of Organic Pollutants from Eutrophic Raw Water by Biological Pretreatment Reactors

Zhu

Yang

Lü

2016

Journal of Chemistry

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Two biological contact oxidation reactors, cascade biofilm reactor (CSBR) and one-step biofilm reactor (OSBR), were used in this paper for pretreatment of eutrophic water from Lake Taihu in China. The CSBR was more effective and stable for eutrophic water treatment than OSBR, in terms of extracellular microcystin-LR,chlorophyll-a, DOC, and biodegradable dissolved organic carbon (BDOC) removal. Removal efficiencies of extracellular microcystin-LR andchlorophyll-a were 75.8% and 59.7% in CSBR and 60.5% and 53.0% after 2 h in OSBR. CSBR had much higher removal efficiency (34.3%) than OSBR (22.7%) for DOC, and CSBR could remove 67% BDOC, accounting for 34% of total DOC in source water. 11.5% of DOC was removed through means other than biological degradation, such as biofilm adsorption and bioflocculation. In CSBR at 5.5~13°C, 57.5% of atrazine was removed at 2 h hydraulic retention time, with background concentration of 136.5 ng/L. Meanwhile, removal efficiencies of three phthalic acid esters (PAEs) (dimethyl phthalate, di-(2-ethylhexyl) phthalate, and di-n-butyl phthalate) were 78.7%, 52.4%, and 85.3%, respectively. Only 35.2% of polycyclic aromatic hydrocarbons (PAHs) could be removed by CSBR with initial concentration of 21.5 μg/L. The results indicated that CSBR is effective in low-molecular-weight organic pollution pretreatment and provides benefits in terms of effluent quality.

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Application of cellular biosensors for detection of atypical toxic bioactivity in microcystin-containing cyanobacterial extracts

Cited by 12 publications

References 46 publications

Toxic mechanisms of microcystins in mammals

Toxic mechanisms of microcystins in mammals

Interaction of Adenosine, Modified Using Carborane Clusters, with Ovarian Cancer Cells: A New Anticancer Approach against Chemoresistance

Removal Characteristics of Organic Pollutants from Eutrophic Raw Water by Biological Pretreatment Reactors

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