Application of Bio-Layer Interferometry for the analysis of protein/liposome interactions

Wallner, Jakob; Lhota, Gabriele; Jeschek, Dominik; Mäder, Alexander; Vorauer-Uhl, Karola

doi:10.1016/j.jpba.2012.10.008

Cited by 70 publications

(55 citation statements)

References 30 publications

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“…3b), indicating strong binding affinity between protein and telodendrimer. However, the dissociation rate was too slow in PBS and not sufficient for dissociation constant ( k off ) fitting[52]. BSA, the most abundant protein in blood plasma, was observed to accelerate telodendrimer dissociation in a concentration dependent manner (Fig.…”

Section: Resultsmentioning

confidence: 99%

Affinity-controlled protein encapsulation into sub-30 nm telodendrimer nanocarriers by multivalent and synergistic interactions

et al. 2016

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Novel nanocarriers are highly demanded for the delivery of heterogeneous protein therapeutics for disease treatments. Conventional nanoparticles for protein delivery are mostly based on the diffusion-limiting mechanisms, e.g., physical trapping and entanglement. We develop herein a novel linear-dendritic copolymer (named telodendrimer) nanocarrier for efficient protein delivery by affinitive coating. This affinity-controlled encapsulation strategy provides nanoformulations with a small particle size (<30 nm), superior loading capacity (>50% w/w) and maintained protein bioactivity. We integrate multivalent electrostatic and hydrophobic functionalities synergistically into the well-defined telodendrimer scaffold to fine-tune protein binding affinity and delivery properties. The ion strength and density of the charged groups as well as the structure of the hydrophobic segments are important and their combinations in telodendrimers are crucial for efficient protein encapsulation. We have conducted a series of studies to understand the mechanism and kinetic process of the protein loading and release, utilizing electrophoresis, isothermal titration calorimetry, Förster resonance energy transfer spectroscopy, bio-layer interferometry and computational methods. The optimized nanocarriers are able to deliver cell-impermeable therapeutic protein intracellularly to kill cancer cells efficiently. In vivo imaging studies revealed cargo proteins preferentially accumulate in subcutaneous tumors and retention of peptide therapeutics is improved in an orthotopic brain tumor, these properties are evidence of the improved pharmacokinetics and biodistributions of protein therapeutics delivered by telodendrimer nanoparticles. This study presents a bottom-up strategy to rationally design and fabricate versatile nanocarriers for encapsulation and delivery of proteins for numerous applications.

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Section: Resultsmentioning

confidence: 99%

Affinity-controlled protein encapsulation into sub-30 nm telodendrimer nanocarriers by multivalent and synergistic interactions

et al. 2016

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“…The binding kinetics were measured on the basis of the attachment of liposomes that increase the thickness of the layer of protein-coated surface that is correlated directly to the spectral shift as measured by biolayer interferometry. 420 This method has considerable high-throughput capability for identification of lead biomolecular drugs. Similarly, viral interactions with receptors on host cell membranes can be mimicked using receptor-bound liposomes.…”

Section: Analytical Applicationsmentioning

confidence: 99%

New Developments in Liposomal Drug Delivery

2015

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“…Using BLI the affinity and kinetics of various interactions can be determined, such as protein-protein, 52 protein-nucleic acid 53 or binding of proteins to liposomes. 54 …”

Section: Bio-layer Interferometrymentioning

confidence: 99%

How to Study Protein-protein Interactions

Podobnik¹,

Kraševec²,

Zavec³

et al. 2016

ACSi

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In memory of prof. dr. Janko Jamnik. AbstractPhysical and functional interactions between molecules in living systems are central to all biological processes. Identification of protein complexes therefore is becoming increasingly important to gain a molecular understanding of cells and organisms. Several powerful methodologies and techniques have been developed to study molecular interactions and thus help elucidate their nature and role in biology as well as potential ways how to interfere with them. All different techniques used in these studies have their strengths and weaknesses and since they are mostly employed in in vitro conditions, a single approach can hardly accurately reproduce interactions that happen under physiological conditions. However, complementary usage of as many as possible available techniques can lead to relatively realistic picture of the biological process. Here we describe several proteomic, biophysical and structural tools that help us understand the nature and mechanism of these interactions.

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Application of Bio-Layer Interferometry for the analysis of protein/liposome interactions

Cited by 70 publications

References 30 publications

Affinity-controlled protein encapsulation into sub-30 nm telodendrimer nanocarriers by multivalent and synergistic interactions

Affinity-controlled protein encapsulation into sub-30 nm telodendrimer nanocarriers by multivalent and synergistic interactions

New Developments in Liposomal Drug Delivery

How to Study Protein-protein Interactions

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