Apo B gene haplotype is associated with lipid profile of higher risk for coronary heart disease in Caucasian Brazilian men

Abstract

We investigated using haplotype analysis whether genetic variation of the apo B gene is associated with a higher risk for coronary heart disease in a Brazilian population. Ins/Del, XbaI, and EcoRI polymorphic sites of the apolipoprotein B (apo B) gene were studied in 67 patients with CHD and in 67 age‐matched healthy individuals selected from a population of Brazilians. The allelic frequency of apo B polymorphisms did not differ between the CHD patients and controls. However, a significant linkage disequilibrium was observed between the XbaI site and Ins/Del polymorphism of the apo B gene in CHD individuals (χ2, P < 0.01). The simultaneous presence of the rare X+ and Del alleles (X+Del haplotype) in males of CHD group was associated with significantly higher serum levels of total cholesterol (P < 0.01), triglycerides (P < 0.05), and LDL‐cholesterol (P < 0.05), and with a higher TC/HDL‐C ratio (P < 0.05). These data indicate that a single haplotype, X+Del, within the apo B gene exerts an impact on lipid metabolism and may contribute to the susceptibility to development of CHD in males from a population of Brazilians. J. Clin. Lab. Anal. 15:19–24, 2001. © 2001 Wiley‐Liss, Inc.

Interaction between SREBP‐1a and APOB polymorphisms influences total and low‐density lipoprotein cholesterol levels in patients with coronary artery disease

“…In the present study, as well as in a previous investigation (21), the Xba I and SP Ins/Del APOB polymorphisms were found to be associated with serum lipid levels in Brazilian populations of European descent. The Xba I polymorphism was also reported to be associated with CAD in Brazilians (23).…”

“…Linkage disequilibrium was observed between the APOB Xba I and SP Ins/Del polymorphisms in both samples (CAD patients D' = 0.70, p < 0.0001; controls D' = 0.72, p < 0.0001). As in previous studies (21, 22), the Xba I+ allele is linked to the SP Del allele.…”

Interaction between SREBP‐1a and APOB polymorphisms influences total and low‐density lipoprotein cholesterol levels in patients with coronary artery disease

“…In the present study, as well as in a previous investigation (21), the Xba I and SP Ins/Del APOB polymorphisms were found to be associated with serum lipid levels in Brazilian populations of European descent. The Xba I polymorphism was also reported to be associated with CAD in Brazilians (23).…”

“…Linkage disequilibrium was observed between the APOB Xba I and SP Ins/Del polymorphisms in both samples (CAD patients D' = 0.70, p < 0.0001; controls D' = 0.72, p < 0.0001). As in previous studies (21, 22), the Xba I+ allele is linked to the SP Del allele.…”

Association of the 12669G>A Apolipoprotein B Gene Polymorphism with Apo-B Serum Level and Lipid Profile in Patients with Coronary Artery Disease Comparing with Individuals Without Coronary Artery Disease in Zanjan Population of Iran

“…Also, in a study in the Italian population which compared 55 Italian patients to 117 control patients, the allele frequencies at EcoRI (E) sites did not differ between groups [23]. Similar results have been reported in the Brazilian population [18,24], Marshal et al [25] and also in South Asian subjects living in Britain [17]. On the other hand, remarkable positive connections in the frequency of the R-allele and CAD has been reported in other population [15,16,26,27].…”

Apolipoprotein B and angiotensin-converting enzyme polymorphisms and aerobic interval training: randomized controlled trial in coronary artery disease patients

“…Considering that this polymorphism cannot directly compromise lipid metabolism, Chiodini et al ( 10 ) and Boekholdt et al ( 27 ) suggested that it may be in linkage disequilibrium with other variants in the ApoB gene itself or nearby, affecting the LDL-receptor-binding region of apolipoprotein B. This linkage disequilibrium has been reported by Machado et al ( 9 ) between two ApoB polymorphisms [( Ins/Del − Insertion/Deletion (rs17240441) and XbaI − X+/X- (rs693)] in a Brazilian population. These authors observed that haplotypes formed by X+ and Del alleles (X+Del haplotype) were associated with significantly higher serum levels of total cholesterol, triglycerides, and LDL-cholesterol.…”

Interaction between SREBP‐1a and APOB polymorphisms influences total and low‐density lipoprotein cholesterol levels in patients with coronary artery disease

“…In the present study, as well as in a previous investigation (21), the Xba I and SP Ins/Del APOB polymorphisms were found to be associated with serum lipid levels in Brazilian populations of European descent. The Xba I polymorphism was also reported to be associated with CAD in Brazilians (23).…”

“…Linkage disequilibrium was observed between the APOB Xba I and SP Ins/Del polymorphisms in both samples (CAD patients D' = 0.70, p < 0.0001; controls D' = 0.72, p < 0.0001). As in previous studies (21, 22), the Xba I+ allele is linked to the SP Del allele.…”

Association of the 12669G>A Apolipoprotein B Gene Polymorphism with Apo-B Serum Level and Lipid Profile in Patients with Coronary Artery Disease Comparing with Individuals Without Coronary Artery Disease in Zanjan Population of Iran

“…Also, in a study in the Italian population which compared 55 Italian patients to 117 control patients, the allele frequencies at EcoRI (E) sites did not differ between groups [23]. Similar results have been reported in the Brazilian population [18,24], Marshal et al [25] and also in South Asian subjects living in Britain [17]. On the other hand, remarkable positive connections in the frequency of the R-allele and CAD has been reported in other population [15,16,26,27].…”

Apolipoprotein B and angiotensin-converting enzyme polymorphisms and aerobic interval training: randomized controlled trial in coronary artery disease patients

“…Considering that this polymorphism cannot directly compromise lipid metabolism, Chiodini et al ( 10 ) and Boekholdt et al ( 27 ) suggested that it may be in linkage disequilibrium with other variants in the ApoB gene itself or nearby, affecting the LDL-receptor-binding region of apolipoprotein B. This linkage disequilibrium has been reported by Machado et al ( 9 ) between two ApoB polymorphisms [( Ins/Del − Insertion/Deletion (rs17240441) and XbaI − X+/X- (rs693)] in a Brazilian population. These authors observed that haplotypes formed by X+ and Del alleles (X+Del haplotype) were associated with significantly higher serum levels of total cholesterol, triglycerides, and LDL-cholesterol.…”

Interaction between SREBP‐1a and APOB polymorphisms influences total and low‐density lipoprotein cholesterol levels in patients with coronary artery disease

“…In the present study, as well as in a previous investigation (21), the Xba I and SP Ins/Del APOB polymorphisms were found to be associated with serum lipid levels in Brazilian populations of European descent. The Xba I polymorphism was also reported to be associated with CAD in Brazilians (23).…”

“…Linkage disequilibrium was observed between the APOB Xba I and SP Ins/Del polymorphisms in both samples (CAD patients D' = 0.70, p < 0.0001; controls D' = 0.72, p < 0.0001). As in previous studies (21, 22), the Xba I+ allele is linked to the SP Del allele.…”

Association of the 12669G>A Apolipoprotein B Gene Polymorphism with Apo-B Serum Level and Lipid Profile in Patients with Coronary Artery Disease Comparing with Individuals Without Coronary Artery Disease in Zanjan Population of Iran

“…Also, in a study in the Italian population which compared 55 Italian patients to 117 control patients, the allele frequencies at EcoRI (E) sites did not differ between groups [23]. Similar results have been reported in the Brazilian population [18,24], Marshal et al [25] and also in South Asian subjects living in Britain [17]. On the other hand, remarkable positive connections in the frequency of the R-allele and CAD has been reported in other population [15,16,26,27].…”

Apolipoprotein B and angiotensin-converting enzyme polymorphisms and aerobic interval training: randomized controlled trial in coronary artery disease patients

“…Considering that this polymorphism cannot directly compromise lipid metabolism, Chiodini et al ( 10 ) and Boekholdt et al ( 27 ) suggested that it may be in linkage disequilibrium with other variants in the ApoB gene itself or nearby, affecting the LDL-receptor-binding region of apolipoprotein B. This linkage disequilibrium has been reported by Machado et al ( 9 ) between two ApoB polymorphisms [( Ins/Del − Insertion/Deletion (rs17240441) and XbaI − X+/X- (rs693)] in a Brazilian population. These authors observed that haplotypes formed by X+ and Del alleles (X+Del haplotype) were associated with significantly higher serum levels of total cholesterol, triglycerides, and LDL-cholesterol.…”