APLNR marks a cardiac progenitor derived with human induced pluripotent stem cells

Lam, Yin‐Yu; Chan, Chun-Ho; Geng, Long; Wong, Nicodemus; Keung, Wendy; Cheung, Yiu‐fai

doi:10.1016/j.heliyon.2023.e18243

Cited by 2 publications

(3 citation statements)

References 39 publications

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“…4A–D ). Similar to our previous study 32 , SHF gene expressions, including ISL1, MEF2C, HAND2 and FGF10 , were found in the sequenced time points (Fig. 3C ).…”

Section: Resultssupporting

confidence: 89%

“…To understand the emergence of TOF-DG-cluster B0, we examined the transcriptomic profile of in vitro cardiac progenitors. Based on our previous study 32 , second heart field (SHF) gene expression peaked on post-differentiation Day (D) 7 and 8. We therefore performed scRNA-seq on hiPSC-cardiac progenitors (hiPSC-CPs) on D7 and D8 and immature hiPSC-CMs on D11 from 2 controls (Control 1 and 2) and 2 TOF- DG (TOF-DG1 and 2) and 1 TOF-ND (TOF-ND1) patients (Fig.…”

Section: Resultsmentioning

confidence: 93%

“…No missense or nonsense mutations of previously reported CHD-related genes were found from any of the hiPSC lines. Cardiac differentiation was accomplished through the APLNR + sorting protocol developed by our laboratory 32 . All hiPSC lines showed satisfactory differentiation efficiency with over 70% TNNT2+ cells.…”

Section: Resultsmentioning

confidence: 99%

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Abnormal developmental trajectory and vulnerability to cardiac arrhythmias in tetralogy of Fallot with DiGeorge syndrome

Chan,

Lam,

Wong

et al. 2023

Commun Biol

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Tetralogy of Fallot (TOF) is the most common cyanotic congenital heart disease. Ventricular dysfunction and cardiac arrhythmias are well-documented complications in patients with repaired TOF. Whether intrinsic abnormalities exist in TOF cardiomyocytes is unknown. We establish human induced pluripotent stem cells (hiPSCs) from TOF patients with and without DiGeorge (DG) syndrome, the latter being the most commonly associated syndromal association of TOF. TOF-DG hiPSC-derived cardiomyocytes (hiPSC-CMs) show impaired ventricular specification, downregulated cardiac gene expression and upregulated neural gene expression. Transcriptomic profiling of the in vitro cardiac progenitors reveals early bifurcation, as marked by ectopic RGS13 expression, in the trajectory of TOF-DG-hiPSC cardiac differentiation. Functional assessments further reveal increased arrhythmogenicity in TOF-DG-hiPSC-CMs. These findings are found only in the TOF-DG but not TOF-with no DG (ND) patient-derived hiPSC-CMs and cardiac progenitors (CPs), which have implications on the worse clinical outcomes of TOF-DG patients.

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“…4A–D ). Similar to our previous study 32 , SHF gene expressions, including ISL1, MEF2C, HAND2 and FGF10 , were found in the sequenced time points (Fig. 3C ).…”

Section: Resultssupporting

confidence: 89%

Section: Resultsmentioning

confidence: 93%