“…Embryos were classified according to their development stage, on a scale from 1 (one cell stage) to 9 (hatched blastocyst stage) and according to their quality as 1 (excellent), 2 (good), 3 (fair) and 4 (degenerate) (Phillips and Jahnke, 2016). For cryopreservation, morula and blastocyst stage embryos with qualities 1 and 2 were placed in a freezing medium with 10% ethylene glycol and 0.3 M sucrose and placed into French straws (Baruselli et al, 2015).…”