Aortic ⍺-smooth muscle actin expressions in aortic disorders and coronary artery disease: An immunohistochemical study

Yuan, Shuai; Wu, Ning

doi:10.14744/anatoljcardiol.2017.7839

Cited by 13 publications

(12 citation statements)

References 20 publications

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“…Lack of α-SMA positive cells in the middle layer of the aortic wall in patients with AAA was observed in comparison to control cells of aorta (Lesiak et al unpublished work). α-SMA expressions in aortic smooth muscle cells of patients with AAA were significantly reduced (Yuan and Wu 2018).…”

Section: Discussionmentioning

confidence: 94%

Searching for new molecular markers for cells obtained from abdominal aortic aneurysm

et al. 2021

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The aim of the study was to investigate specific potential markers for cells obtained from three layers of human AAA divided into three segments along the AAA based on morphological differences. The isolated cells were compared to control commercial cell types from healthy human abdominal aortas. For each type of aortic layer, three specimens from 6 patients were compared. Total RNA was isolated from 36 cell cultures for gene expression profiling and potential new cytometry markers were typed. Isolated cells were analyzed by flow cytometry by using fluorochrome-conjugated antibodies to markers: CNN1, MYH10, ENG, ICAM2, and TEK. The relative expression of 45 genes in primary cell cultures and control lines was analyzed. Statistically significant differences were found in the expression of most of the analyzed genes between individual layers and control lines. Based on relative expression, antibodies were selected for flow cytometry. Gene expression profiles allowed to select new potential cytometry markers: CNN1, MYH10, MYOCD, ENG, ICAM2, TEK. However, none of the tested markers seems to be optimal and characteristic for a specific layer of AAA.

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Section: Discussionmentioning

confidence: 94%

Searching for new molecular markers for cells obtained from abdominal aortic aneurysm

et al. 2021

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“…Mean ± SD 3 ± 3 2.1 ± 1.6 3.9 ± 5.3 4.9 ± 3.9 3.7 ± 4.4 4 ± 6.1 3.5 ± 2.9 2.7 ± 1.6 8.7 ± 1 Cells that were obtained from patients with AAA were analyzed for their phenotypes using flow cytometry and markers specific for the particular expected types of cells. The markers used in our work were selected based on reports by numerous authors who have demonstrated results of immunohistochemical analyses of the aortic wall, showing these specific cells in different parts of the aorta [12][13][14]. Flow cytometry data of cells from the IL of AAAs showed that these cells did not show CD31 or vWF antigens.…”

Section: Cd68mentioning

confidence: 99%

Characteristic of cells isolated from human Abdominal Aortic Aneurysm samples cultured in vitro

et al. 2022

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Introduction:This study aimed to standardize cell culture methods for major cell types isolated from three layers of human AAA. We also aimed to determine cell types in each layer of each AAA segment and compare them with cell types in layers of control, unchanged segments. Material and methods: We divided AAAs into three segments along the AAA and control segments flanking the aneurysm. Isolated cells following expansion were analyzed by flow cytometry, immunochemistry, and microscopic methods. Fluorochrome-conjugated antibodies were used to detect the three major cell types (endothelial cells, smooth muscle cells, and fibroblasts) in each layer of every AAA segment. Results: The culture of cells from the three AAA segments was successfully established in 21% of patients. In all of the layers, only a small proportion of cells showed layer-specific markers of cell types. The majority of cells from every layer were positive for CD90, which is considereda specific marker of fibroblasts in the aorta. Conclusions: We describe a methodology for isolation of cells, their culture conditions, and phenotypic characterization for AAA. The wall of AAA loses its specific types of cells in all of the layers compared with the normal abdominal aortic wall.

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“…In a normal individual, VSMCs of the aorta demonstrate a well-differentiated, contractile state; in atherosclerosis, the VSMCs expressed increased amount of the ⍺-smooth muscle actin (SMA) indicating a shift toward the synthetic phenotype leading to a narrowing in the lumen. In patients with aortic aneurysms, VSMCs expressed decreased amount of the ⍺-smooth muscle actin (SMA) indicating a severe SMC degeneration or a switch between the contractile and synthetic phenotype of SMCs, which may be even bene icial because of the production of an extracellular matrix that is necessary for healing of the vascular wall [23].…”

Section: Pathogenesismentioning

confidence: 99%

Association of Coronary Artery Disease and Abdominal Aortic Aneurysm – A Narrative Review

A¹

2021

RIJCCM

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Studies have shown a strong association between coronary artery disease (CAD) and Abdominal Aortic Aneurysm (AAA). CAD is an independent predictor for developing AAA. The strong risk factors which were associated with the development of AAA were older age, male sex, hypertension, smoking, dyslipidemia, respiratory disease, cerebrovascular disease, claudication, and renal insuffi ciency in predicting the development of AAA. The prevalence of AAA among patients with angiographyverifi ed CAD was higher in men. It also increased to 8.6% in men aged above 65 years. They also found that 2.5% of patients with normal coronary profi le, 4.3% of patients with single vessel disease, 5.7% of patients with double vessel disease and 14.4% of patients with triple vessel disease on angiogram had AAA. The Pathological features like chronic infl ammation, degradation of the extracellular matrix and apoptosis of the vascular smooth muscle cells are common to both CAD and AAA. The vascular Smooth Muscle Cell (VSMC) plays an important role in the pathogenesis of coronary artery disease and aortic aneurysms. Another mechanism identifi ed in these VSMCs is the role of ubiquitin-like containing PHD and RING fi nger domains 1 (UHRF1) as the epigenetic master regulator of VSMC plasticity. Large symptomatic AAA with signifi cant CAD, a combined procedure should be the preferred approach. Asymptomatic AAA and CAD, a staged approach of CABG followed by AAA repair within two weeks should be performed to minimize the risk of AAA rupture. A one-time ultrasound screening for AAAs in men or women 65 to 75 years of age with a history of tobacco use, in fi rst-degree relatives of patients who present with an AAA, in men or women older than 75 years with a history of tobacco use is recommended.

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Aortic ⍺-smooth muscle actin expressions in aortic disorders and coronary artery disease: An immunohistochemical study

Cited by 13 publications

References 20 publications

Searching for new molecular markers for cells obtained from abdominal aortic aneurysm

Searching for new molecular markers for cells obtained from abdominal aortic aneurysm

Characteristic of cells isolated from human Abdominal Aortic Aneurysm samples cultured in vitro

Association of Coronary Artery Disease and Abdominal Aortic Aneurysm – A Narrative Review

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