Anything but Conventional Chromatography Approaches in Bioseparation

Roque, Ana C. A.; Pina, Ana Sofia; Azevedo, Ana M.; Aires-Barros, M. Raquel; Jungbauer, Alois; Profio, Gianluca Di; Heng, Jerry Y. Y.; Haigh, Jonathan; Ottens, Marcel

doi:10.1002/biot.201900274

Cited by 58 publications

(56 citation statements)

References 95 publications

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“…Technology Readiness Level (TRL) Index, introduced by NASA (Héder, 2017), can be used to assess the stage toward large-scale implementation where certain technology is located. The TRL index scale goes from 1 to 9, where TRL 1 concerns to fundamental concept/research, TRL 2-4 to technological research, TRL 5-8 to product demonstration, and TRL 9 to implementation (Roque et al, 2020).…”

Section: Future Prospectsmentioning

confidence: 99%

A Structured Approach to Recover Valuable Compounds from Agri-food Side Streams

Moreno-González

Ottens

2021

Food Bioprocess Technol

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Food side streams contain useful compounds such as proteins, sugars, polyphenols, and amino acids that might get discarded during processing. The concentration of these components may be low (e.g., fruit side streams are mainly composed by water, around 90%, while polyphenol content in rapeseed meal is less than 3% dry weight) and therefore effective separation techniques should be evaluated. The aim of this review is to identify the different process steps (like pretreatment, volume reduction, phase change, solid removal, purification, and formulation) required to recover high-value products from agri-food residues. It reviews different plant-based byproducts as sources (cereal bran, fruit pomace, oilseed meals, fruit wastewater) of valuable compounds and discusses the relevant technologies required for processing (such as extraction, adsorption, crystallization, drying, among others). A structured approach to design recovery processes presented focused on high purity products. This work demonstrates that multiple high-value products can be recovered from a single agri-food side stream depending on the processing steps and the origin source (strong and soft structures and wastewater).

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Section: Future Prospectsmentioning

confidence: 99%

A Structured Approach to Recover Valuable Compounds from Agri-food Side Streams

Moreno-González

Ottens

2021

Food Bioprocess Technol

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“…In this way, comparing a precipitation assay with the current protein A chromatography capture step, the lower costs associated (15–140 € per liter precipitant vs 5 000–14 000 € per liter protein A resin), [ 5 ] the scalability of the precipitation process already implemented in the downstream pipeline of biopharmaceuticals, [ 43,44 ] and the potential scale and implementation of magnetic fishing processes under cGMP equipment, [ 45 ] makes affinity magnetic precipitation a potential new platform for the capture of high added value proteins directly from the complex cell supernatant.…”

Section: Discussionmentioning

confidence: 99%

Magnetic Precipitation: A New Platform for Protein Purification

Santos

Iria

Manuel

et al. 2020

Biotechnology Journal

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One of the trends in downstream processing comprises the use of “anything‐but‐chromatography” methods to overcome the current downfalls of standard packed‐bed chromatography. Precipitation and magnetic separation are two techniques already proven to accomplish protein purification from complex media, yet never used in synergy. With the aim to capture antibodies directly from crude extracts, a new approach combining precipitation and magnetic separation is developed and named as affinity magnetic precipitation. A precipitation screening, based on the Hofmeister series, and a commercial precipitation kit are tested with affinity magnetic particles to assess the best condition for antibody capture from human serum plasma and clarified cell supernatant. The best conditions are obtained when using PEG3350 as precipitant at 4 °C for 1 h, reaching 80% purity and 50% recovery of polyclonal antibodies from plasma, and 99% purity with 97% recovery yield of anti‐TNFα mAb from cell supernatants. These results show that the synergetic use of precipitation and magnetic separation can represent an alternative for the efficient capture of antibodies.

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“…Stemming from the rapid growth in demand, significant improvements in upstream processing, such as more efficient fermentation and cell culture technologies, lead to an increase in titre production from an average 0.2 g/L in 1985 to 2.56 g/L in 2014. This shifted the bottleneck in manufacturing towards the downstream processing which heavily relies on costly multi-step packed-bed chromatography (2) and accounts for a total of up to 70% of the total production costs (3). Crystallisation as a purification technique is already widely adapted in the manufacturing of small molecules.…”

Section: Introductionmentioning

confidence: 99%