Anxiolytic-like effect of chalcone N-{4’[(2E)-3-(3-nitrophenyl)-1-(phenyl)prop-2-en-1-one]} acetamide on adult zebrafish (Danio rerio): Involvement of the 5-HT system

Ferreira, Maria Kueirislene Amâncio; Silva, Antônio Wlisses da; Silva, Francisca Crislândia Oliveira; Neto, Antônio Eufrásio Vieira; Campos, Adriana Rolim; Santos, Sacha Aubrey Alves Rodrigues; Teixeira, Alexandre Magno Rodrigues; Xavier, Jayze da Cunha; Bandeira, Paulo Nogueira; Nogueira, C.E.S.; Brito, Débora Hellen Almeida de; Rebouças, Emanuela L.; Magalhães, Francisco Ernani Alves; Menezes, Jane Eire Silva Alencar de; Santos, Hélcio Silva dos

doi:10.1016/j.bbrc.2020.03.129

Cited by 20 publications

(8 citation statements)

References 31 publications

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“…In a study whose objective was to show the anxiolytic effects of chalcones using diazepam as a standard, the results showed that all doses caused motor impairment in relation to diazepam, showing a decrease in the locomotor activity of zebrafish, suggesting that chalcones and diazepam are compounds that decrease locomotor activity in zebrafish. Thus, proving that the open field directs toward more specific analyses for anxiety tests 34,35 .…”

Section: Most Pesticides Tend To Increase Locomotion Activity Inmentioning

confidence: 95%

Anxiolytic effects of Leishmania braziliensis proteins in a zebrafish animal model

et al. 2023

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Introduction. Leishmaniasis is among the 22 most neglected diseases worldwide. Few information is related about the evidence of neurological involvement that leishmaniasis can cause due to the ulcerations formed in the skin along short and long term. However, some authors have cited that leishmaniasis can cause inflammation in the central nervous system, which can trigger neuropsychomotor processes such as anxiety. Objective. The present study focuses on the evaluation of the effects of total Leishmania proteins on the central nervous system in the Zebrafish model. Method. Leishmania was initially cultured to extract total proteins by Sonication and Bradford methods. After total protein extraction, the zebrafish were inoculated to observe the symptomatology caused by protein load, and after seven days of inoculation, we performed the behavioral tests (Open Field and Bright & Dark). Results. The fish treated with Leishmania protein showed several cutaneous alterations, as well as behavioral changes. The open field behavioral tests showed a high number of crosses compared to the control group. In the light and dark test, the zebra fish had a longer stay in the light field, and an anxiolytic action was observed in the animals that received the protein administration. Conclusion. The total Leishmania proteins may have an anxiolytic action in the zebrafish model.

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Section: Most Pesticides Tend To Increase Locomotion Activity Inmentioning

confidence: 95%

Anxiolytic effects of Leishmania braziliensis proteins in a zebrafish animal model

et al. 2023

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“…To investigate the anxiolytic effect in the GABAergic system 47 , the GABA A receptor structure, identified as “CryoEM structure of human full-length alpha1beta3gamma2L GABA A in complex with diazepam (Valium), GABA A , and megabody Mb38″ 48 , was used. The protein structure was obtained from the Protein Data Bank repository ( ), which was deposited under the code PDB 6HUP.…”

Section: Methodsmentioning

confidence: 99%

Indole Alkaloids of Rauvolfia ligustrina and Their Anxiolytic Effects in Adult Zebrafish

et al. 2023

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Rauvolfia species are well-known as producers of bioactive monoterpene indole alkaloids (MIAs) which exhibit a broad spectrum of biological activities. A new vobasine-sarpagan-type bisindole alkaloid (1) along with six known monomeric indoles (2, 3/4, 5, and 6/7) were isolated from the ethanol extract from the roots of Rauvolfia ligustrina. The structure of the new compound was elucidated by interpretation of their spectroscopic data (1D and 2D NMR and HRESIMS) and comparison with published data for analog compounds. The cytotoxicity of the isolated compounds was screened in zebrafish (Danio rerio) model. The possible GABAergic (diazepam: positive control) and serotoninergic (fluoxetine: positive control) mechanisms of action in adult zebrafish were also evaluated. No compounds were cytotoxic. Compound 2 and the epimers 3/4 and 6/7 showed mechanism action by GABAA, while compound 1 by serotonin receptor (anxiolytic activity). Molecular docking studies showed that compounds 2 and 5 have a greater affinity by GABAA receptor when compared with diazepam, whereas 1 showed the best affinity for 5HT2AR channel when compared to risperidone.

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“…increased the time spent in the white compartment in the light/dark chamber. Cyproheptadine, a 5-HT 2A receptor antagonist, and pizotifen, a 5-HT 1 and 5-HT 2A/2C receptor antagonist attenuated the anxiolytic-like effect of PAAMNBA (Ferreira et al, 2020 ). This evidence supports an equivalent neuropharmacological substrate between zebrafish and rodents in regulating anxiety-like behaviors, suggesting that zebrafish is valuable as rodents in behavioral pharmacology investigations of anxiety.…”

Section: Neurobiology Of Anxiolytic Effects In Zebrafishmentioning

confidence: 99%

Zebrafish as a Useful Tool in the Research of Natural Products With Potential Anxiolytic Effects

Cueto‐Escobedo

Germán-Ponciano

Guillén-Ruiz

et al. 2022

Front. Behav. Neurosci.

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Zebrafish (Danio rerio) is a popular and valuable species used in many different biomedical research areas. The complex behavior that fish exhibit in response to different stimuli allows researchers to explore the biological and pharmacological basis of affective and mood disorders. In this sense, anxiety is commonly studied in preclinical research with animal models in rodents. During the last decade, those models have been successfully adapted to zebrafish. Stressful stimuli, such as novel environments, chemical substances, light conditions, and predator images, can trigger defensive behaviors considered indicators of an anxiety-like state. In the first stage, models were adapted and validated with different stressors and anxiolytic drugs with promising results and are now successfully used to generate scientific knowledge. In that sense, zebrafish allows several routes of administration and other methodological advantages to explore the anxiolytic effects of natural products in behavioral tests as novel tank, light-dark chamber, and black/white maze, among others. The present work will review the main findings on preclinical research using adult zebrafish to explore anxiolytics effects of natural products as plant secondary metabolites such as flavonoids, alkaloids and terpenes or standardized extracts of plants, among others. Scientific literature confirms the utility of zebrafish tests to explore anxiety-like states and anxiolytic-like effects of plant secondary metabolites, which represent a useful and ethical tool in the first stages of behavioral.

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Anxiolytic-like effect of chalcone N-{4’[(2E)-3-(3-nitrophenyl)-1-(phenyl)prop-2-en-1-one]} acetamide on adult zebrafish (Danio rerio): Involvement of the 5-HT system

Cited by 20 publications

References 31 publications

Anxiolytic effects of Leishmania braziliensis proteins in a zebrafish animal model

Anxiolytic effects of Leishmania braziliensis proteins in a zebrafish animal model

Indole Alkaloids of Rauvolfia ligustrina and Their Anxiolytic Effects in Adult Zebrafish

Zebrafish as a Useful Tool in the Research of Natural Products With Potential Anxiolytic Effects

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