Ants (Hymenoptera: Formicidae) as Carriers of Fungi in Hospital Environments: An Emphasis on the Genera &lt;I&gt;Tapinoma&lt;/I&gt; and &lt;I&gt;Pheidole&lt;/I&gt;

Pantoja, Lydia Dayanne Maia; Filho, Renato Evando Moreira; Brito, Érika Helena Salles de; Aragão, Tiago Bessa; Brilhante, Raimunda Sâmia Nogueira; Cordeiro, Rossana de Aguiar; Rocha, Marcos F.G.; Monteiro, André Jalles; Quinet, Yves; Sidrim, José Júlio Costa

doi:10.1603/033.046.0423

Cited by 17 publications

(15 citation statements)

References 33 publications

(26 reference statements)

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“…Ascomycetes dominate the mycobiota recovered from typical indoor environments (2), as humans, insects, and other animals contribute significantly to this population, both as vectors and as hosts (2,46). The extent to which these observations carry over to cleanrooms and other facilities kept very clean is less clear, however.…”

Section: Discussionmentioning

confidence: 99%

Pyrosequencing-Derived Bacterial, Archaeal, and Fungal Diversity of Spacecraft Hardware Destined for Mars

Duc

Vaishampayan

Nilsson

et al. 2012

Appl Environ Microbiol

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ABSTRACTSpacecraft hardware and assembly cleanroom surfaces (233 m2in total) were sampled, total genomic DNA was extracted, hypervariable regions of the 16S rRNA gene (bacteria and archaea) and ribosomal internal transcribed spacer (ITS) region (fungi) were subjected to 454 tag-encoded pyrosequencing PCR amplification, and 203,852 resulting high-quality sequences were analyzed. Bioinformatic analyses revealed correlations between operational taxonomic unit (OTU) abundance and certain sample characteristics, such as source (cleanroom floor, ground support equipment [GSE], or spacecraft hardware), cleaning regimen applied, and location about the facility or spacecraft. National Aeronautics and Space Administration (NASA) cleanroom floor and GSE surfaces gave rise to a larger number of diverse bacterial communities (619 OTU; 20 m2) than colocated spacecraft hardware (187 OTU; 162 m2). In contrast to the results of bacterial pyrosequencing, where at least some sequences were generated from each of the 31 sample sets examined, only 13 and 18 of these sample sets gave rise to archaeal and fungal sequences, respectively. As was the case for bacteria, the abundance of fungal OTU in the GSE surface samples dramatically diminished (9× less) once cleaning protocols had been applied. The presence of OTU representative of actinobacteria, deinococci, acidobacteria, firmicutes, and proteobacteria on spacecraft surfaces suggests that certain bacterial lineages persist even following rigorous quality control and cleaning practices. The majority of bacterial OTU observed as being recurrent belonged to actinobacteria and alphaproteobacteria, supporting the hypothesis that the measures of cleanliness exerted in spacecraft assembly cleanrooms (SAC) inadvertently select for the organisms which are the most fit to survive long journeys in space.

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Section: Discussionmentioning

confidence: 99%

Pyrosequencing-Derived Bacterial, Archaeal, and Fungal Diversity of Spacecraft Hardware Destined for Mars

Duc

Vaishampayan

Nilsson

et al. 2012

Appl Environ Microbiol

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“…represented 25% of the isolates in our study, which probably reflects the environmental condition under which this research was conducted, considering that the species belonging to this genus, along with Penicillium spp. and Aspergillus spp., represent common airborne fungi in indoor and outdoor environments (Solomon et al , 2006; Pantoja et al , 2009; Miao et al , 2010).…”

Section: Discussionmentioning

confidence: 99%

Yeasts from Macrobrachium amazonicum: a focus on antifungal susceptibility and virulence factors of Candida spp.

Brilhante

Paiva

Sampaio

et al. 2011

FEMS Microbiology Ecology

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In the present study, it was sought to compare yeast microbiota of wild and captive Macrobrachium amazonicum and evaluate the antifungal susceptibility and production of virulence factors by the recovered isolates of Candida spp. Additionally, cultivation water was monitored for the presence of fungi. Overall, 26 yeast isolates belonging to three genera and seven species were obtained, out of which 24 were Candida spp., with Candida famata as the most prevalent species for both wild and captive prawns. From cultivation water, 28 isolates of filamentous fungi were obtained, with Penicillium spp., Cladosporium spp. and Aspergillus spp. as the most frequent genera. Eight out of 24 Candida spp. isolates were resistant to azole derivatives, out of which four were recovered from wild-harvested prawns. As for production of virulence factors, three (12.5%) and eight (33.3%) isolates presented phospholipase and protease activity, respectively. This is the first comparative study between wild and captive prawns and the first report on yeast microbiota of M. amazonicum. The most relevant finding was the high percentage of resistant Candida spp., including from wild individuals, which suggests the occurrence of an environmental imbalance in the area where these prawns were captured.

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“…9,11 Alguns autores usam iscas como atrativos para amostragem de formigas, mas este método não foi utilizado no presente estudo, uma vez que Os trabalhadores eram sempre fáceis de localizar, e a intenção era evitar introduzir no experimento outras fontes de fungos, causando contaminação. 15 Isolamento e identificação de fungos Geral e específico chaves taxonômicas foram utilizadas para identificação. 16,20 A diversidade de espécies de formigas encontradas neste estudo é comparável ao que foi observado em estudos anteriores que relataram um intervalo de uma a 23 espécies.…”

Section: Introductionunclassified

Formigas Como Vetores De Bactérias E Fungos No Interior De Um Hospital Do Sul Da Bahia

Almeida¹,

Theodoro²,

Zetoles³

et al. 2019

Democratização Do Conhecimento E Valorização Profissional:caminhos Para O Desenvolvimento

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Resumo Infecções hospitalares, causadas principalmente por bactérias e fungos, constituem causa crescente de morbidade e mortalidade no Brasil e no mundo. Embora alguns insetos sejam vetores reconhecidos de microrganismos, as formigas têm-se revelado como ameaças à saúde pública, atuando como vetores mecânicos de microrganismos patogênicos. Assim, conhecer as espécies de formigas, bem como identificar as espécies de microrganismos que são encontrados e transportados por esses insetos, podem subsidiar ações educativas, complementando programas de prevenção e controle das infecções hospitalares. Foram realizadas coletas em diferentes setores de um hospital público da cidade de Itabuna nos meses de junho a agosto de 2009. Vinte e cinco formigas operárias foram coletadas. Também foi feita coleta, com o auxílio de um swab estéril, do local no qual os insetos foram retiradas. As formigas coletadas foram colocadas no interior de tubos estéreis, numeradas e imediatamente enviadas aos laboratórios de Mimercologia da CEPLAC para identificação e Microbiologia da UESC para processamento, o qual consistiu na inoculação de formigas inteiras em Ágar Sabouraud para crescimento fúngico e na separação das formigas em cabeça, tórax, abdome, patas e antenas, as quais foram colocadas individualmente em tubos com caldo BHI para crescimento bacteriano. Em seguida as bactérias foram inoculadas nos meios de

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Ants (Hymenoptera: Formicidae) as Carriers of Fungi in Hospital Environments: An Emphasis on the Genera <I>Tapinoma</I> and <I>Pheidole</I>

Cited by 17 publications

References 33 publications

Pyrosequencing-Derived Bacterial, Archaeal, and Fungal Diversity of Spacecraft Hardware Destined for Mars

Pyrosequencing-Derived Bacterial, Archaeal, and Fungal Diversity of Spacecraft Hardware Destined for Mars

Yeasts from Macrobrachium amazonicum: a focus on antifungal susceptibility and virulence factors of Candida spp.

Formigas Como Vetores De Bactérias E Fungos No Interior De Um Hospital Do Sul Da Bahia

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