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“…culture as already described [10]. Briefly, tissues were dissociated with collagenase (50 mg per 100 ml) in the presence of DNase I (1 mg per 100 ml) in Hepes buffer at pH 7.3.…”
Section: Animals and Reagentsmentioning
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“…culture as already described [10]. Briefly, tissues were dissociated with collagenase (50 mg per 100 ml) in the presence of DNase I (1 mg per 100 ml) in Hepes buffer at pH 7.3.…”
Section: Animals and Reagentsmentioning
“…The various substances to be tested were included in the culture media from the first day and throughout, with media being changed daily. That period of treatment was required to register clear-cut effects on CBG biosynthesis by hepatocytes in culture [10] and actually reflects the time lag likewise seen in i o in rats administered with corticosterone and thyroxine or after adrenalectomy [18]. Cellular DNA contents were measured by the bis-benzimide technique [19] and taken as an index of cell number.…”
Section: Animals and Reagentsmentioning
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