Antiviral Effect of Aqueous Extracts from Species of the Lamiaceae Family against <i>Herpes simplex</i> Virus Type 1 and Type 2 <i>in vitro</i>

Nolkemper, Silke; Reichling, Jürgen; Stintzing, Florian C.; Carle, Reinhold; Schnitzler, Paul

doi:10.1055/s-2006-951719

Cited by 154 publications

(118 citation statements)

References 14 publications

(20 reference statements)

Supporting

Mentioning

105

Contrasting

Unclassified

Order By: Relevance

“…Formula to determine the virus inhibition percentage: Nolkemper et al (2006) also reported the antiviral action on both types of HSV, HSV-1 and HSV-2, in experiments using spices that presented significant reduction of these virus replications in RC -37 cells.…”

Section: Resultsmentioning

confidence: 99%

“…Extracts from plants, algae and essential oils from seeds or fruits presenting compounds with antiviral effects have been the most studied items within this category. Flavonoid compounds such as quercetin revealed decrease or inhibition of the yield of Herpes simplex virus (HSV 1), and also of type 2 virus, thanks to a probable virucidal action, as the result was an inhibition of viral DNA replication (Nolkemper et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Inhibition of DNA virus: Herpes-1 (HSV-1) in cellular culture replication, through an antioxidant treatment extracted from rosemary spice

Mancini

Torres

Pinto

et al. 2009

Braz. J. Pharm. Sci.

View full text Add to dashboard Cite

This work aimed to evaluate antiviral properties in antioxidants from spices. Phenolic compounds extracted from rosemary (Rosmarinus officinallis, L) by hot water, had their antioxidant activity determined by spectrophotometry using β carotene/linoleic acid system. The rosemary extract was evaluated by antiviral assay of Herpes Virus type-1 (HSV-1) replication in VERO cells, in the presence or absence of the spice. 10,000 TCID 50 /mL of the HSV-1 was kept for 3 h at 4 o C, with 300 ppm of rosemary extract, and 100 ppm of butyl hydroxyl toluene (BHT). Then, these viruses were inoculated in VERO cells incubated at 37 o C in CO 2 -5 %, for seven days. Daily, they were examined and the end point was based on 100% of CPE in virus control (without antioxidants). The HSV-1 replication inhibition percentage (IP) measured the antiviral action from antioxidants, showing viral reductions of the 82.0, 82.5%, in the presence of rosemary and rosemary + BHT, respectively. As an extension, cell test corresponded to the similar viral decrease (IP = 85.0 and 86.3%) in both aforementioned situations. Results lead to conclude that phenolic compounds from rosemary revealed an antiviral action on herpesvirus-1.Uniterms: Natural antioxidants. Virus replication. Phenolic compounds. Herpesvirus. Rosemary.Neste estudo foi avaliada a ação antiviral de antioxidantes de especiaria. Extrato aquoso de alecrim (Rosmarinus officinalis, L), que apresentou atividade antioxidante através de espectrofotometria usando o sistema β caroteno/ácido linoléico, foi avaliado em ensaios com vírus herpes-1 na replicação em células VERO. Nestes ensaios foram utilizados 10.000 TCID 50%/mL do vírus HSV-1, mantidos em contato com 300 ppm do extrato de alecrim e com 100 ppm de butil hidroxi tolueno (BHT), durante 3h a 4°C. Esses vírus, em seguida, foram inoculados em células VERO incubadas a 37 °C/5% de CO 2 por sete dias. Pelo efeito citopático (ECP) e o "end point" de ECP do controle de vírus (sem antioxidante), foi possível observar que houve reduções na replicação viral de 82 e 82,5% na presença do alecrim e do alecrim + BHT, respectivamente. Nessa situação,avaliou-se ainda a redução da adsorção viral às células, que apresentou índices similares de 85,0 e 86,3% de redução na capacidade da adsorção. Estas reduções no desempenho do HSV foram medidas pela fórmula de porcentagem de inibição da replicação viral (PI). Os resultados levam a concluir que os compostos fenólicos do alecrim apresentam ação antiviral sobre o HSV-1.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Inhibition of DNA virus: Herpes-1 (HSV-1) in cellular culture replication, through an antioxidant treatment extracted from rosemary spice

Mancini

Torres

Pinto

et al. 2009

Braz. J. Pharm. Sci.

View full text Add to dashboard Cite

show abstract

“…Bioactivity: Aqueous extract of Prunella vulgaris showed a high antiviral activity against Herpes Simplex Virus-1, 2 and Acyclovir (resistant) strain of HSV (Nolkemper et al, 2006). P-374 herbal therapy for endometriosis of Prunella vulgaris (Self heal) reduces the size and number of enomedriotic xenograft in immune deficient RAG -2/gamma (C) knockout mice (Collins et al, 2006).…”

Section: Use In Tsmmentioning

confidence: 99%

An overview and economical importance of few selected endangered medicinal plants grown in Jammu and Kashmir region of India

Rasool¹,

Khan²,

Hamid³

et al. 2016

View full text Add to dashboard Cite

Medicinal plants as a group comprise approximately 8000 species and account for around 50% of all the higher flowering plant species of India. Millions of rural households use medicinal plants in a self-help mode. Over one and a half million practitioners of the Indian System of Medicine in the oral and codified streams use medicinal plants in preventive, promotive and curative applications. In recent years, the growing demand for herbal product has led to a quantum jump in volume of plant materials traded within and across the countries. Further, in view of the side effects of synthetic drugs coupled with their high cost of production, medicinal plants have become major sectors of trade and commerce and are significantly contributing to the socioeconomic developments of certain developed and developing countries. Though India has a rich biodiversity, the growing demand is putting a heavy strain on the existing resources. Among various States of India, Jammu and Kashmir State has a rich diversity of medicinal plants because it provides a diverse type of habitats for their growth. Kashmir valley represents the temperate region, Jammu area represents sub-tropical and tropical and Ladakh as cold desert region. More than 50% of plant based medicines prescribed in British Pharmacopoeia are growing in Jammu and Kashmir State.In the present paper, 13 important medicinal plants, viz., Achillea millefolium L., Aconitum heterophyllum Wall.ex Royle, Artemisia absinthium L., Asplenuim adiantum-nigrum L., Berberis lycium Royle, Cydonia oblonga Miller, Delphinium denudatum Wall.ex.H&T, Picrorhiza kurroa Royle.ex.Benth, Po dop hyllum hex and rum Royle, Prune lla vu lga ris L., Sa lix cap rea L., Taraxacum officinale Webber. and Viola odorata L. with attributes to traditional system of medicine and their use by local population in primary health care have been described. The vernacular names, distribution pattern, folk use, use in traditional system of medicine (TSM), phytochemical constituents isolated and characterized in each plant species are discussed. The present status, in State of nature of each plant species based on ethnobotanical exploration made by authors from time to time and future prospects for commericial cultivation is discussed.

show abstract

“…This activity is attributed to chemical constituents, such as rosmarinic acid, caffeic acid, chlorogenic acid, and ferulic acid, which inhibit viral DNA and RNA replication (Allahverdiyev et al, 2004;Jassim, Naji, 2003;British Herbal Pharmacopoeia, 1996;Dimitrova, Dimov, Manaloval, 1993). Moreover, results suggest that constituents of Melissa officinalis extract interact with structures of the virion envelope during the extracellular phase which are necessary for adsorption or entry into host cells, displaying the highest antiviral activity in vitro against HSV-1 and HSV-2 (inhibitory concentrations of 0.025 mg/mL and 0.027 mg/mL respectively) (Nolkemper et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Mechanical properties and total hydroxycinnamic derivative release of starch/glycerol/Melissa officinalis extract films

Rechia

Morona

Zepon

et al. 2010

Braz. J. Pharm. Sci.

View full text Add to dashboard Cite

The aim of this study was to investigate the mechanical properties of starch/glycerol/Melissa officinalis, a topical drug delivery system for labial herpes treatment. Four films were prepared with different concentrations of starch, glycerol, and Melissa officinalis extract. The results revealed that increasing the glycerol concentration in the film reduced elasticity modulus and tensile strength, exhibiting a plasticizing effect. The increase in free volume resulted in increased release of hydroxycinnamic derivatives expressed as rosmarinic acid. Uniterms

show abstract

Antiviral Effect of Aqueous Extracts from Species of the Lamiaceae Family against Herpes simplex Virus Type 1 and Type 2 in vitro

Cited by 154 publications

References 14 publications

Inhibition of DNA virus: Herpes-1 (HSV-1) in cellular culture replication, through an antioxidant treatment extracted from rosemary spice

Inhibition of DNA virus: Herpes-1 (HSV-1) in cellular culture replication, through an antioxidant treatment extracted from rosemary spice

An overview and economical importance of few selected endangered medicinal plants grown in Jammu and Kashmir region of India

Mechanical properties and total hydroxycinnamic derivative release of starch/glycerol/Melissa officinalis extract films

Contact Info

Product

Resources

About