Antitussive, expectorant and anti-inflammatory activities of four alkaloids isolated from Bulbus of Fritillaria wabuensis

Wang, Dongdong; Wang, Shu; Chen, Xiong; Xu, Xueqing; Zhu, Junkai; Nie, Lihuan; Long, Xian-Jun

doi:10.1016/j.jep.2011.10.036

Cited by 129 publications

(104 citation statements)

References 22 publications

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“…Antitussive effects were investigated with the mouse cough model induced by ammonia liquor (Chen, 1994;Wang et al, 2012). The mice were exposed to a 500 mL glass jar sprayed with 13% ammonium hydroxide (0.2 mL) to record the latent period and cough frequency.…”

Section: Evaluation Of Antitussive Activitymentioning

confidence: 99%

Antitussive, expectorant, and anti-inflammatory activities of four caffeoylquinic acids isolated fromTussilago farfara

Zhao

Xiang

et al. 2015

Pharmaceutical Biology

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Context: The flower bud of Tussilago farfara L. (Compositae) (FTF) is one of the traditional Chinese medicinal herbs used to treat cough, phlegm, bronchitic, and asthmatic conditions. Objective: The objective of this study is to isolate four caffeoylquinic acids from the ethyl acetate extract (EtE) of FTF and to evaluate their antitussive, expectorant, and anti-inflammatory activities. Materials and methods: The structures of compounds 1-4 isolated from EtE were determined by spectral analysis. Mice were orally treated with these compounds and their mixture (in a ratio of 5:28:41:26 as in EtE) at doses of 10 and 20 mg/kg once daily for 3 d. The antitussive and expectorant activities were evaluated separately with the ammonia liquor-induced model and the phenol red secretion model. The anti-inflammation activity was evaluated using leukocyte count in the bronchoalveolar lavage fluid after ammonia liquor-induced acute airway inflammation. Results: The four compounds were identified as chlorogenic acid (1), 3,5-dicaffeoylquinic acid (2), 3,4-dicaffeoylquinic acid (3), and 4,5-dicaffeoylquinic acid (4). All compounds, especially compound 4 (58.0% inhibition in cough frequency), showed a significant antitussive effect. However, the mixture was the most effective to inhibit the cough frequency by 61.7%. All compounds also showed a significant expectorant effect, while compound 2 was the most potent to enhance the phenol red secretion by 35.7%. All compounds significantly alleviated inflammation, but compound 4 showed the strongest effect to inhibit the leukocytosis by 49.7%. Discussion and conclusion: The caffeoylquinic acids and their mixture, exhibiting significant antitussive, expectorant, and anti-inflammatory effects, could be considered as the main effective ingredients of FTF, and they may act in a collective and synergistic way. ARTICLE HISTORY

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Section: Evaluation Of Antitussive Activitymentioning

confidence: 99%

Antitussive, expectorant, and anti-inflammatory activities of four caffeoylquinic acids isolated fromTussilago farfara

Zhao

Xiang

et al. 2015

Pharmaceutical Biology

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“…Antitussive effects were investigated by using a classical mouse cough model induced by ammonia liquor with minor modifications (Han et al, 2010;Wang et al, 2012). Mice of either sex were randomly divided into 8 groups of 10 mice each and treated as Section 2.3 (except for group 2 and 3).…”

Section: Antitussive Effect Assessmentmentioning

confidence: 99%

Antitussive, expectorant and anti-inflammatory activities of different extracts from Exocarpium Citri grandis

Jiang

Song

Wang

et al. 2014

Journal of Ethnopharmacology

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“…Antitussive effects were evaluated using a classical model induced by ammonia liquor in mice according to the reported method (Wang et al, 2012). In brief, each mouse was placed in a 1000 ml special glass chamber with the addition of 0.3 ml 25% NH 4 OH via oral administration of the test compounds for 60 min.…”

Section: Antitussive Effects Against Ammonia Induced Coughingmentioning

confidence: 99%

Antitussive and expectorant activities ofPotentilla anserina

Guo

Wei

Jian

2015

Pharmaceutical Biology

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ContextThe root of Potentilla anserina L. (Rosaceae) is an herbal medicine that has been used as an antitussive and expectorant drug for thousands of years in Chinese folk medicine. Objective: This study estimated the antitussive and expectorant effects of P. anserina extract to validate its traditional use. Materials and methods The antitussive and expectorant activities of the ethanol extract, aqueous extract, and polysaccharides from P. anserina were evaluated using classical animal models.Results The results showed that in three antitussive tests, the aqueous extract and polysaccharides at high and low doses significantly inhibited the frequency of cough induced by ammonia and sulfur dioxide in mice and by citric acid in guinea pigs, and increased the latent period of cough in guinea pigs. Similarly, the aqueous extract and polysaccharides also showed significant expectorant activity compared with the control in phenol red secretion experiments. Polysaccharides at dose of 600 mg/kg enhanced tracheal phenol red output by 121.1%, the ammonium chloride (positive control) at dose of 1000 mg/kg by 117.4%. However, the ethanol extract at a high dose (600 mg/kg) has antitussive activity only in the sulfur dioxide induced coughing test. Moreover, the polysaccharides at the same dose showed better bioactivity than the aqueous extract in all tests. Discussion and conclusion The results of the present study provide evidence that P. anserina can be used as an antitussive and expectorant herbal medicine and that polysaccharides may be the main active ingredients of P. anserina responsible for its bioactivities. ARTICLE HISTORY

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Antitussive, expectorant and anti-inflammatory activities of four alkaloids isolated from Bulbus of Fritillaria wabuensis

Cited by 129 publications

References 22 publications

Antitussive, expectorant, and anti-inflammatory activities of four caffeoylquinic acids isolated fromTussilago farfara

Antitussive, expectorant, and anti-inflammatory activities of four caffeoylquinic acids isolated fromTussilago farfara

Antitussive, expectorant and anti-inflammatory activities of different extracts from Exocarpium Citri grandis

Antitussive and expectorant activities ofPotentilla anserina

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