Antitumor activity of Moringa oleifera (drumstick tree) flower trypsin inhibitor (MoFTI) in sarcoma 180-bearing mice

Patriota, Leydianne Leite de Siqueira; Ramos, Dalila de Brito Marques; Santos, Angela Caroline Lima Amorim dos; Silva, Yasmym Araújo; Silva, Mariana Gama e; Torres, Diego José Lira; Procópio, Thamara Figueiredo; Oliveira, Alisson Macário de; Coelho, Luana Cassandra Breitenbach Barroso; Pontual, Emmanuel Viana; Silva, Diego César Nunes da; Lorena, Virgínia Maria Barros de; Mendes, Rosemairy Luciane; Napoleão, Thiago Henrique

doi:10.1016/j.fct.2020.111691

Cited by 21 publications

(7 citation statements)

References 43 publications

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“…The genotoxic damage found for PEAh is supported by the findings of other studies, since it has been reported that protein extract and pure lectins in plants present genotoxic effect, demonstrated by micronucleus, ames, and comet assays (Faheina-Martins et al, 2011;Patriota et al, 2020;Rolim et al, 2011).…”

Section: Hourssupporting

confidence: 83%

“…These differences may be due to the species and the analytical methods employed (Correa et al, 1986;Valdes-Rodriguez et al, 1993). It has been demonstrated that this type of protease inhibitors present cytotoxic effects in some malignant and normal cell lines, such as MCF-7, HeLa, A549, HEK (Lanza et al, 2004;Patriota et al, 2020;Shamsi et al, 2017) and can also cause hypertrophy and hyperplasia of the pancreas, and lead to adenomas and carcinomas of the exocrine pancreas in rats (Hathcock, 1991). This supports the results obtained, since trypsin inhibitors were found in the amaranth extract, which may be contributing to the cytotoxic and toxicological effects in animals.…”

Section: Hoursmentioning

confidence: 99%

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Phytochemical, cytotoxic, and genotoxic evaluation of protein extract of Amaranthus hypochondriacus seeds

Valadéz-Vega

Lugo-Magaña

Morales-González

et al. 2021

CyTA - Journal of Food

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Amaranth has been of interest for its nutritional quality and its multiple benefits; however, there is little research on its toxicological effect. This work studied antinutritional compounds, the cytotoxic and genotoxic effect in the Amaranthus protein extract (PEAh). The content of lectins, tannins, saponins, and trypsin inhibitors was determined. Biological studies were conducted to determine toxicity in male CD-1 mice by intraperitoneal administration. The genotoxic potential was determined by the micronucleus test. The bone marrow cytotoxicity was determined by polychromatic erythrocyte (PE) rate and the cytotoxic effect was evaluated by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) test. Our results showed considerably high concentrations of lectins and trypsin inhibitors, lower concentrations of tannins and saponins in PEAh. The PEAh was toxic to mice, presenting genotoxic and cytotoxic damage to bone marrow and hepatocytes.Evaluación fitoquímica, citotóxica y genotóxica del extracto de proteína de semillas de Amaranthus hypochondriacus RESUMEN El amaranto ha sido de interés por su calidad nutricional y sus múltiples beneficios; sin embargo, hay poca investigación sobre su efecto toxicológico. Este trabajo estudió compuestos antinutricionales, el efecto citotóxico y genotóxico en el extracto de proteína de Amaranthus hypochondriacus (PEAh). Se determinó el contenido de lectinas, taninos, saponinas e inhibidores de tripsina. Se realizaron estudios biológicos para determinar la toxicidad en ratones machos CD-1 mediante administración intraperitoneal. El potencial genotóxico se determinó mediante la prueba de micronúcleos. La citotoxicidad de la médula ósea se determinó mediante la tasa de eritrocitos policromáticos (PE) y el efecto citotóxico se evaluó mediante la prueba MTT. Los resultados mostraron concentraciones considerablemente altas de lectinas e inhibidores de tripsina, concentraciones más bajas de taninos y saponinas en PEAh. El PEAh fue tóxico para los ratones, presentando daño genotóxico y citotóxico en la médula ósea y los hepatocitos.

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Section: Hourssupporting

confidence: 83%

Section: Hoursmentioning

confidence: 99%

Phytochemical, cytotoxic, and genotoxic evaluation of protein extract of Amaranthus hypochondriacus seeds

Valadéz-Vega

Lugo-Magaña

Morales-González

et al. 2021

CyTA - Journal of Food

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“…Reviewed evidence from Asare and co-workers revealed that Moringa oleifera is genotoxic at supra-supplementation levels of 3,000 mg/kg body weight, with intake mostly considered is safe at levels ≤1,000 mg/kg (body weight) ( Asare et al, 2012 ). However, other studies have indicated that although available literature is very promising ( Awodele et al, 2012 ; Ajibade et al, 2013 ; Stohs and Hartman, 2015 ; Patriota et al, 2020 ; Siddiqui et al, 2021 ; Teshome et al, 2021 ), additional clinical (human) to accomplish standardized extracts of this plant.…”

Section: Safety and The Toxicity Profile Of Moringa Oleiferamentioning

confidence: 99%

A Review on the Antidiabetic Properties of Moringa oleifera Extracts: Focusing on Oxidative Stress and Inflammation as Main Therapeutic Targets

et al. 2022

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Moringa oleifera is one of the popular plants that have shown significant health benefits. Certainly, preclinical evidence (predominantly from animal models) summarized in the current review supports the beneficial effects of Moringa oleifera leaf extracts in combating the prominent characteristic features of diabetes mellitus. This includes effective control of blood glucose or insulin levels, enhancement of insulin tissue sensitivity, improvement of blood lipid profiles, and protecting against organ damage under sustained conditions of hyperglycemia. Interestingly, as major complications implicated in the progression of diabetes, including organ damage, Moringa oleifera leaf and seed extracts could efficiently block the detrimental effects of oxidative stress and inflammation in these preclinical models. Notably, these extracts (especially leaf extracts) showed enhanced effects in strengthening intracellular antioxidant defences like catalase, superoxide dismutase, and glutathione to lower lipid peroxidation products and reduce prominent pro-inflammatory markers such as tumor necrosis factor-α, interleukin (1L)-β, IL-6, monocyte chemoattractant protein-1 and nitric oxide synthase. From animal models of diabetes, the common and effective dose of leaf extracts of Moringa oleifera was 100–300 mg/kg, within the treatment duration of 2–8 weeks. Whereas supplementation with approximately 20 g leaf powder of Moringa oleifera for at least 2 weeks could improve postprandial blood glucose in subjects with prediabetes or diabetes. Although limited clinical studies have been conducted on the antidiabetic properties of Moringa oleifera, current findings provide an important platform for future research directed at developing this plant as a functional food to manage diabetic complications.

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“…With relatively limited cancer studies on other parts of M. oleifera, Patriota et al [58] treating sarcoma 180-bearing mice with trypsin inhibitor from M. oleifera flower found that it can cause significant tumor weight loss (90.1-97.9%). Al-Asmari et al [59] found that bark extracts showed significant anti-cancer properties for MDA-MB-231 and HCT-8 cancer cells and had significantly lower cell survival in both cell lines, while the number of apoptotic cells in cancer cell lines increased significantly.…”

Section: Oleifera: a Promising Anti-cancer Agentmentioning

confidence: 99%

Anti-Cancer and Medicinal Potentials of Moringa Isothiocyanate

Lau

2021

Molecules

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Moringa oleifera (M. oleifera), which belongs to the Moringaceae family, is a common herb, rich in plant compounds. It has a variety of bioactive compounds that can act as antioxidants, antibiotics, anti-inflammatory and anti-cancer agents, etc., which can be obtained in different body parts of M. oleifera. Isothiocyanates (ITCs) from M. oleifera are one class of these active substances that can inhibit cancer proliferation and promote cancer cell apoptosis through multiple signaling pathways, thus curbing cancer migration and metastasis, at the same time they have little adverse effect on normal cells. There are multiple variants of ITCs in M. oleifera, but the predominant phytochemical is 4-(α-L-rhamnosyloxy)benzyl isothiocyanate, also known as moringa isothiocyanate (MIC-1). Studies have shown that MIC-1 has the possibility to be used clinically for the treatment of diabetes, neurologic diseases, obesity, ulcerative colitis, and several cancer types. In this review, we focus on the molecular mechanisms underlying the anti-cancer and anti-chronic disease effects of MIC-1, current trends, and future direction of MIC-1 based treatment strategies. This review combines the relevant literature of the past 10 years, in order to provide more comprehensive information of MIC-1 and to fully exploit its potentiality in the clinical settings.

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Antitumor activity of Moringa oleifera (drumstick tree) flower trypsin inhibitor (MoFTI) in sarcoma 180-bearing mice

Cited by 21 publications

References 43 publications

Phytochemical, cytotoxic, and genotoxic evaluation of protein extract of Amaranthus hypochondriacus seeds

Phytochemical, cytotoxic, and genotoxic evaluation of protein extract of Amaranthus hypochondriacus seeds

A Review on the Antidiabetic Properties of Moringa oleifera Extracts: Focusing on Oxidative Stress and Inflammation as Main Therapeutic Targets

Anti-Cancer and Medicinal Potentials of Moringa Isothiocyanate

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