Antistress Effects of the Ethanolic Extract fromCymbopogon schoenanthusGrowing Wild in Tunisia

Abstract

This study aimed to investigate the antistress properties of the ethanol extract of Cymbopogon schoenanthus (CSEE), growing wild in the southern part of Tunisia. The effect of extracts on H2O2-induced cytotoxicity and stress in human neuroblastoma SH-SY5Y cells. Its effect on stress-induced in ICR mice was exposed to force swim and tail suspension, in concordance with heat shock protein expression (HSP27 and HSP90), corticosterone, and catecholamine neurotransmitters level. Our results demonstrated that pretreatment of SH-SY5Y cells with CSEE at 1/2000, 1/1000, and 1/500 v/v dilutions significantly inversed H2O2-induced neurotoxicity. Moreover, CSEE treatments significantly reversed heat shock protein expression in heat-stressed HSP47-transformed cells (42°C, for 90 min) and mRNA expression of HSP27 and HSP90 in H2O2-treated SH-SY5Y. Daily oral administration of 100 mg/kg and 200 mg/kg CSEE was conducted to ICR mice for 2 weeks. It was resulted in a significant decrease of immobility time in forced swimming and tail suspension tests. The effect of CSEE on animal behavior was concordant with a significant regulation of blood serum corticosterone and cerebral cortex levels of catecholamine (dopamine, adrenaline, and noradrenaline). Therefore, this study was attempted to demonstrate the preventive potential of CSEE against stress disorders at in vitro and in vivo levels.

Antidepressant‐Like Effect of Ferulic Acid via Promotion of Energy Metabolism Activity

“…CSE treatment decreased immobility time of mice in both models. The effect of CSE on animal behavior was concordant with a significant reduction of corticosterone in serum and upregulation of neurotransmitters such as dopamine, adrenaline, and noradrenaline levels in the cerebral cortex of mice . We also analyzed active compounds in CSE using HPLC analysis, which identified several active compounds in CSE such as gallic acid, quercetin‐3‐rhamnoside, and ferulic acid (FA).…”

“…We also analyzed active compounds in CSE using HPLC analysis, which identified several active compounds in CSE such as gallic acid, quercetin‐3‐rhamnoside, and ferulic acid (FA). In particular, FA is the most abundant polyphenol in CSE composing up to 10% of the total phenolic constituent …”

Detoxification capacity and protective effects of medicinal plants (part 2): plant based review

“…The results also demonstrated that pretreatment of neuroblastoma SH-SY5Y cells with CSEE at 1/2000, 1/1000, and 1/500 v/v dilutions, significantly inversed H 2 O 2 -induced neurotoxicity. Moreover, CSEE treatments significantly reversed heat shock protein expression in heat-stressed HSP47-transformed cells (42 °C, for 90 min) and mRNA expression of HSP27 and HSP90 in H 2 O 2 -treated SH-SY5Y [122].…”

Chemical composition and spasmolytic activity of Cymbopogon schoenanthus (L.) Spreng. (Poaceae) essential oil from Sudan

“…This plant is used as a culinary herb in salads and traditional meat dishes. Because of its pleasant aroma, it is also consumed as a refreshing beverage prepared by steeping the aerial parts in hot water [7]. Its medicinal properties were considered helpful in the treatment of gout, prostate inflammation, kidney disorders, stomach pain, fever and rheumatism [6,8].…”

“…Furthermore, C. schoenanthus is traditionally used as a digestive, for treating intestinal spasms as well as for treating anorexia, i.e. for increasing appetite [7,9].…”