Antisense vicR-Loaded Dendritic Mesoporous Silica Nanoparticles Regulate the Biofilm Organization and Cariogenicity of Streptococcus mutans

Spermine-starch nanoparticles with antisense vicR suppress Streptococcus mutans cariogenicity

“…In our recently published work, 17 we constructed the aminated dendritic mesoporous silica nanoparticles (DMSNs-NH 2 ) carrying plasmid-AS vicR . The plasmid-AS vicR loaded on DMSNs-NH2 could be transformed into S. mutans , which reduced EPS synthesis and biofilm organization and showed preliminary anti-caries potential.…”

“…For treatment, a sterile cotton bud (Labor Import) was used to swab the rat teeth with 200 μL of SSN (1 mg mL −1 ), AS vicR plasmid (0.12 mg mL −1 ) or SSN-AS vicR (1 mg mL −1 ) suspensions respectively on days 1 to 14 as per our recently published study. 17 Rats were weighed once a week and then their physical appearance was routinely recorded. On day 28, the animals were sacrificed by CO 2 asphyxiation.…”

“…The starch vector can also be hydrolyzed by amylase in saliva, thus achieving gene delivery in an innovative manner. In our recently published work, 17 we constructed the aminated dendritic mesoporous silica nanoparticles (DMSNs-NH 2 ) carrying plasmid-AS vicR . However, the use of DMSNs-NH 2 is restricted by its non-degradable properties.…”

Delivery of Therapeutic Biopolymers Employing Silica-Based Nanosystems

“…Another interesting example of local administration was reported by Tian et al, who developed a dendritic silica nanosystem able to deliver an anti- Streptococcus mutans plasmid [ 67 ]. Employed particles were prepared with cyclohexane as pore expander and an amino-modified surface to ensure electrostatically binding of the plasmid.…”

“…In conclusion, plasmid delivery has also been employed to treat bacterial infections. Through a downregulation of extracellular polysaccharides in Streptococcus Mutans colonies, Tian et al were able to reduce the cariogenic potential of this type of cocci [ 67 ]. Their model was built by electrostatic deposition of the negatively charged anti-VicR plasmid onto dendritic amino-modified MSNs.…”