Antiribosomal P protein antibodies in Chilean SLE patients: no association with renal disease

Abstract

The objective of this work was to determine the frequency and clinical associations of anti-ribosomal P protein antibodies (Anti-P) in a cohort of Chilean patients with systemic lupus erythematosus (SLE). Between 1996 and 1998, 141 consecutive patients with SLE were examined prospectively according with a standard protocol. Disease activity was measured by MEX-SLEDAI in 138 patients. Anti-P positivity was determined by double immune diffusion or Western blot and ELISA. Anti-P was found in 21 (15%) patients. In the Anti-P positive patients recent onset SLE (disease duration of 1 year or less) was more frequent (P = 0.018). Anti-P was found in 23% of 83 patients with active SLE vs 4% of the 55 patients with inactive SLE (Yates corrected P = 0.00479). An association with anti-dsDNA antibodies by Farr assay was observed. Anti-P positive patients had a median Farr of 65 IU/ml (1.4-1240) and Anti-P negative of 12 IU/ml (1.4-992; P-value = 0.0084). During the study only two patients had lupus psychosis and they were Anti-P positive. No association was found with liver disease (six patients, two with Anti-P antibodies) or active glomerulonephritis (22 patients four with Anti-P). Our data shows that the presence of Anti-P antibodies supports the clinical diagnosis of lupus psychosis.

Schwann Cell to Axon Transfer of Ribosomes: Toward a Novel Understanding of the Role of Glia in the Nervous System

“…Teased fibers were blocked/permeabilized in PBS containing 5% fish skin gelatin (Sigma) and 0.2% Triton X-100 for 1 h at room temperature and incubated overnight in the same solution with the following primary antibodies: human anti-ribosome antiserum, 1:1000 [from a patient carrying a systemic lupus erythematosus characterized by Massardo et al (2002), a kind gift from S. Jacobelli, Pontificia Universidad Cató lica, Santiago, Chile]; mouse anti-neurofilament (clone N52; Sigma), 1:1000; rabbit anti-S100 (Dako), 1:200; rabbit anti-peripheral myelin glycoprotein P0 (a kind gift from J. P. Brockes, Ludwig Institute for Cancer Research, London, UK), 1:600; rabbit anti-myelin basic protein (MBP; Stem Cell Technologies), 1:200; rabbit anti-GFP (AbCam ab290), 1:500; and human anti-ribosomal P antigen (ImmunoVision), 1:100. After washing, the preparations were incubated for 2.5 h at room temperature with the appropriate secondary antisera; FITC-donkey anti-mouse IgG1, 1:200; TRITC-goat anti-human IgG, 1:200 (both from Jackson ImmunoResearch); AlexaFluor 647-goat anti-rabbit IgG, 1:400; AlexaFluor 647-goat anti-mouse IgG1, 1:400 (both from Molecular Probes), washed three times in PBS, mounted in Vectashield (Vector Laboratories) and viewed with a Bio-Rad radiance 2000 confocal microscope.…”

Actualización en lupus neuro-psiquiátrico con énfasis en déficit cognitivo

“…Se encuentran de 5% a 13% de los pacientes con LES. Son específicos de LES, y se han relacionado con LESNP en globo (psicosis lúpica y trastornos depresivos), pero con una potencia predictora dé-bil 21,34,[44][45][46][47][48] . Recientemente, Matus et al, trabajando en nuestro laboratorio, identificaron un autoanticuerpo proveniente de una paciente con psicosis lúpica dirigido contra un antígeno P ubicado en la superficie neuronal (llamado NSPA por sus siglas en inglés), reconocido por autoanticuerpos anti-P 49 .…”

Anticorpo antiproteína P ribossomal em pacientes com hepatite autoimune

“…A maior frequência de cirrose no grupo anti-P positivo reforça o potencial papel patogênico desse anticorpo no fígado, assim como nas manifestações psiquiátricas no LES 29 , na nefrite lúpica 30,31 e na hepatite associada ao LES 12,16,32 . Estudos prévios demonstraram a presença da proteína ribossomal P0 em células de hepatoma 33 e a habilidade do anti-P penetrar nestas células e causar disfunção celular 14 .…”