Antiproliferative potential of astaxanthin-rich alga Haematococcus pluvialis Flotow on human hepatic cancer (HepG2) cell line

Subramani, Nagaraj; Rajaram, M.G.; Arulmurugan, P.; Baskaraboopathy, A; Karuppasamy, K.; Jayappriyan, K.R.; Sundararaj, R; Rengasamy, Ramasamy

doi:10.1016/j.bionut.2012.03.009

Cited by 38 publications

(25 citation statements)

References 30 publications

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“…We can conclude that synthetic and natural astaxanthins are not toxic to HUVECs up to the concentration of 15 µM. These results were consistent with those of the literature, in particular with the study of Nagaraj et al [ 35 ]. In this study, the authors performed the same protocol, but using HepG2 and MCF-7 cancer cells, which should be more resistant to toxic drugs.…”

Section: Resultssupporting

confidence: 93%

“…Cells had the same elongated shape as the control. According to Nagaraj et al [ 35 ], the mechanism of toxicity of astaxanthin is dual: an increase in the permeability of the membrane and an interaction with microtubules resulting in the blockage of the mitotic cycle for high concentrations (>40 µM). Therefore, the absence of morphological changes of HUVEC at 10 μM confirmed the absence of toxicity of astaxanthin at this concentration.…”

Section: Resultsmentioning

confidence: 99%

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Astaxanthin from Haematococcus pluvialis Prevents Oxidative Stress on Human Endothelial Cells without Toxicity

Bastias²,

et al. 2015

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Astaxanthin, a powerful antioxidant, is a good candidate for the prevention of intracellular oxidative stress. The aim of the study was to compare the antioxidant activity of astaxanthin present in two natural extracts from Haematococcus pluvialis, a microalgae strain, with that of synthetic astaxanthin. Natural extracts were obtained either by solvent or supercritical extraction methods. UV, HPLC-DAD and (HPLC-(atmospheric pressure chemical ionization (APCI)+)/ion trap-MS) characterizations of both natural extracts showed similar compositions of carotenoids, but different percentages in free astaxanthin and its ester derivatives. The Trolox equivalent antioxidant capacity (TEAC) assay showed that natural extracts containing esters displayed stronger antioxidant activities than free astaxanthin. Their antioxidant capacities to inhibit intracellular oxidative stress were then evaluated on HUVEC cells. The intracellular antioxidant activity in natural extracts was approximately 90-times higher than synthetic astaxanthin (5 µM). No modification, neither in the morphology nor in the viability, of vascular human cells was observed by in vitro biocompatibility study up to 10 µM astaxanthin concentrations. Therefore, these results revealed the therapeutic potential of the natural extracts in vascular human cell protection against oxidative stress without toxicity, which could be exploited in prevention and/or treatment of cardiovascular diseases.

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Section: Resultssupporting

confidence: 93%

Section: Resultsmentioning

confidence: 99%

Astaxanthin from Haematococcus pluvialis Prevents Oxidative Stress on Human Endothelial Cells without Toxicity

Bastias²,

et al. 2015

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“…The results also demonstrated the cytotoxic activity of H. pluvialis on MCF7 human caucasian breast adenocarcinoma and on HePG 2 human hepatocellular carcinoma cell line that were also in favour of the carotenoid fraction. The antiproliferative effect of astaxanthin‐rich H. pluvialis extract on HePG 2 human hepatocellular carcinoma cell line was previously reported by Nagaraj et al (). The effect on colon cancer was far more promising than the effects on the other cell lines indicating selective activity, this is also apparent from the diminished effect of the polar fraction on colon cancer cell line in comparison with the effects on other cell lines.…”

Section: Discussionsupporting

confidence: 58%

Cytotoxic activity of carotenoid rich fractions from Haematococcus pluvialis and Dunaliella salina microalgae and the identification of the phytoconstituents using LC‐DAD/ESI‐MS

Baz

Hussein

Mahmoud

et al. 2017

Phytotherapy Research

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Microalgae represent a rich source that satisfies the growing need for novel ingredients of nutriceuticals, pharmaceuticals, and food supplements. Haematococcus pluvialis and Dunaliella salina microalgae are isolated from the Egyptian hydro-flora and are reported for their potent antioxidant activities. The cytotoxic activity of different fractions of both microalgae was investigated on 4 cell lines HePG2, MCF7, HCT116, and A549. The carotenoid rich fraction of H. pluvialis showed potent cytotoxic activity against colon cancer cell line and moderate activity against both liver and breast cancer cell lines. On the other hand, the carotenoid rich fraction of D. salina showed mild cytotoxic activity on breast and liver cancer cell lines. The carotenoid rich fraction of H. pluvialis was analysed using LC-DAD/ESI-MS and the major carotenoids were identified either free as well as bounded to fatty acids.

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“…Tranilast has been suggested to perturb intercellular Ca 2+ equilibrium (Shishibori et al, 1999), which may account for the significant decline in brain and liver lipid peroxidation product, MDA. In context, depletion of GSH and SOD has been reported to markedly enhance lipid peroxidation (Nagaraj et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

Tranilast reduces serum IL-6 and IL-13 and protects against thioacetamide-induced acute liver injury and hepatic encephalopathy

Abdеlaziz

Elkashef

Said

2015

Environmental Toxicology and Pharmacology

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Antiproliferative potential of astaxanthin-rich alga Haematococcus pluvialis Flotow on human hepatic cancer (HepG2) cell line

Cited by 38 publications

References 30 publications

Astaxanthin from Haematococcus pluvialis Prevents Oxidative Stress on Human Endothelial Cells without Toxicity

Astaxanthin from Haematococcus pluvialis Prevents Oxidative Stress on Human Endothelial Cells without Toxicity

Cytotoxic activity of carotenoid rich fractions from Haematococcus pluvialis and Dunaliella salina microalgae and the identification of the phytoconstituents using LC‐DAD/ESI‐MS

Tranilast reduces serum IL-6 and IL-13 and protects against thioacetamide-induced acute liver injury and hepatic encephalopathy

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