1989
DOI: 10.1007/bf01871778
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Abstract: We developed site-directed rabbit antisera against synthetic peptides selected from the deduced amino acid sequence of the hamster lung beta 2-adrenergic receptor (amino acids 16-31 and 174-189, respectively). All antisera directed against peptide 1 (four of four rabbits) as well as two antisera directed against peptide 2 (two of four rabbits) recognized the purified beta 2-adrenergic receptor in immunoblot conditions when used at a dilution of 1:500. Antisera directed against peptide 1 as well as peptide 2 we… Show more

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Cited by 5 publications
(2 citation statements)
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“…Furthermore pure brain SRIF receptor was not used as an antigen since insufficient quantities of pure receptor are available to generate antibodies (9). Because of the micropurification procedure (18,19), antibodies reacting with a limited population of proteins could be obtained. In the case of F4, only antibodies reacting with proteins of55-60 kDa were selected.…”
Section: Resultsmentioning
confidence: 99%
“…Furthermore pure brain SRIF receptor was not used as an antigen since insufficient quantities of pure receptor are available to generate antibodies (9). Because of the micropurification procedure (18,19), antibodies reacting with a limited population of proteins could be obtained. In the case of F4, only antibodies reacting with proteins of55-60 kDa were selected.…”
Section: Resultsmentioning
confidence: 99%
“…The binding of [3-adrenergic agonists to these cell surface receptors leads to the stimulation of adenylate cyclase activity and the accumulation of intracelluar cyclic AMP. In order to further characterize 13-adrenergic receptors, it was of interest to raise anti-receptor antibody (2)(3)(4)(5)(6).…”
Section: Introductionmentioning
confidence: 99%