Antioxydant Extract of Endophytic Fungi Fusarium oxysporum LBKURCC41

Marlinda, Sri; Teruna, Hilwan Yuda; Pratiwi, Nova Wahyu; Ardhi, Aulia; Saryono, Saryono

doi:10.31258/jnat.17.2.1-9

Cited by 3 publications

(2 citation statements)

References 4 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…can increase the resistance of tomato plants because it produces secondary metabolites that act as antioxidants. According to Marlinda et al (2019), secondary metabolites, ethyl acetate in the endophytic fungus Fusarium sp. can act as antibacterials.…”

Section: Resultsmentioning

confidence: 99%

Potential of Endophytic Fusarium sp. Against Bacterial Wilt Disease in Tomato Plants

2024

Nusantara Science and Technology Proceedings

View full text Add to dashboard Cite

Ralstonia solanacearum is a pathogen that causes bacterial wilt in tomato plants (Lycopersicum esculentum), can decrease production until 90%. The controls generally use chemical pesticides which are harmful to the environment and humans, so it is necessary alternative controls using endophytic Fusarium sp. The aim of the research was to determine the effect of endophytic Fusarium sp. to bacterial wilt. Endophytic Fusarium sp. was explored on tomato plant in Tlekung, Junrejo-Batu and taken systematically randomly then brought to the laboratory. Isolation of endophytic fungi was done by washing the plants with running water, cutting 1cm then was sterilized. The sample was planted on Potato Dextrose Agar (PDA) and incubated at 37°C for 48 hours, if its growth was purified on new media. Isolation of R. solanacearum was done by washing the stems, cutting 0.5 cm then sterilizing. The piece is put into a test tube, added 10 ml of sterile water until the bacterial mass comes out and dissolves in sterile water. The mass of bacteria in sterile water is shaken using vortex until suspension forms. The suspension was grown on TZC agar and incubated at 28 0 C for 24 hours, if it grow was purified on new media. Application of endophytic Fusarium sp. carried out using immersing technique of the roots of tomato plant. Inoculation of R. solanacearum was done into sterile soil before the plant were planted. The study used completely randomized design (CRD) with control and isolate of endophytic Fusarium sp. as treatments with 3 repeat and conducted in the greenhouse of the Faculty of Agriculture UPN "Veteran" East Java. Observations are incubation period and disease intensity, data were analyzed using BNT 5%. The results show that endophytic Fusarium sp. delayed the period incubation and suppressed the intensity of bacterial wilt disease in tomato plants.

show abstract

Section: Resultsmentioning

confidence: 99%

Potential of Endophytic Fusarium sp. Against Bacterial Wilt Disease in Tomato Plants

2024

Nusantara Science and Technology Proceedings

View full text Add to dashboard Cite

show abstract

“…that have weak potential may need more time incubation to produce the compounds and that do not show antibacterial potential in S. aureus may have other potential compounds. Several studies have shown that endophytic microbes can produce antifungal substances (Souza, et al, 2014), antioxidants (Marlinda et al, 2019), anticancer, antivirus, insecticide (Lodewyckx, et al, 2002), and IAA hormone (Yarnaliza et al, 2010) Based on its effectiveness, the antibacterial activity of endophytic bacteria from the roots of the Purwoceng (P. pruatjan Molk.) was categorized as a bacteriostatic group because it can inhibit the growth S. aureus that was used.…”

Section: Discussionmentioning

confidence: 99%

Screening of Endophytic Bacteria from Purwoceng Roots (Pimpinella pruatjan Molk.) Antibacterial Activity Against Staphylococcus aureus

2021

Journal of Biological Science and Education

View full text Add to dashboard Cite

A. IntroductionPurwoceng (Pimpinella pruatjan Molk.) is a commercial herbal plant with Coumarin, Sterols, Alkaloids, Saponins, and several types of oligosaccharides in the roots (Darwati et al., 2006). Secondary metabolite compounds produced by purwoceng have abilities such as antimicrobial, anticoagulant, and anti-inflammatory (Darwati et al., 2006, Widayat et al., 2012.The ability of purwoceng to produce antimicrobials can be used to reduce the growth of pathogenic bacteria. Staphylococcus aureus is a type of pathogenic bacteria that often cause infectious diseases in the community (

show abstract

Selection of Non-Mycotoxigenic Inulinase Producers in the Group of Black Aspergilli for Use in Food Processing

Stojanovic

Stepanović

Špirović-Trifunović

et al. 2022

Food Technol. Biotechnol. (Online)

View full text Add to dashboard Cite

Research background. Inulinases are used for fructooligosaccharide production and they are research topic for scientific community and for industry too. Black aspergilli represent a diverse group of species which has use for enzyme production, in particular some species are known as potent inulinases producers. Finding new potential producers from the environment is as important as improving production with known strains. Safety use of enzymes provided by aspergilli in food industry is placed ahead of their benefit for inulinase production. Experimental approach. Here we shown specific approach to finding/screening of newly isolated fungal inulinase producers which combine newly developed screening method and an equally important assessment of the toxigenic potential of the fungus. In this study 39 black aspergilli collected from different substrates in Serbia were identified and assessed for inulinases production. Results and conclusions. The most common species were Aspergillus tubingensis (51.2 %), followed by A. niger (23.1 %), A. welwitschiae (23.1 %), and A. uvarum (2.6 %). Selection of the isolates for inulinases production was conducted using a cheap and easy, fast and non-hazardous alternative inulinase screening test developed in this work. For selected inulinases producing strains enzymatic activity was confirmed spectrophotometrically. Since some A. niger and A. welwitschiae are able to produce mycotoxins ochratoxin A (OTA) and fumonisins (FB), the toxigenic potential of selected inulinases producers was assessed analytically and genetically. Fungal enzyme producer could be considered as food safety only after crossing results of both approaches for toxic potential - the direct presence of mycotoxins in the enzyme preparation (analytically) and a presence of genes clusters (genetically). In some strains absence of OTA and FB production capability was molecularly confirmed by absence of complete or critical parts of biosynthetic gene clusters, respectively. The two best inulinases producers and mycotoxin non-producers (without mycotoxin production capability as additional safety) were selected as potential candidates for further development of enzyme production. Novelty and scientific contribution. Presented innovative approach for selection of potential fungal enzyme producer shows that only non-toxigenic fungi could be considered as useful in food industry. Although this study was done on local isolates, the approach is applicable globally.

show abstract

Antioxydant Extract of Endophytic Fungi Fusarium oxysporum LBKURCC41

Cited by 3 publications

References 4 publications

Potential of Endophytic Fusarium sp. Against Bacterial Wilt Disease in Tomato Plants

Potential of Endophytic Fusarium sp. Against Bacterial Wilt Disease in Tomato Plants

Screening of Endophytic Bacteria from Purwoceng Roots (Pimpinella pruatjan Molk.) Antibacterial Activity Against Staphylococcus aureus

Selection of Non-Mycotoxigenic Inulinase Producers in the Group of Black Aspergilli for Use in Food Processing

Contact Info

Product

Resources

About