Antioxidative Collagen-Derived Peptides In Human-Placenta Extract

Togashi, Shin-ichi; Takahashi, Noriko; Iwama, Masanori; Watanabe, Satoshi; Tamagawa, Kayoko; Fukui, Tetsuya

doi:10.1053/plac.2002.0833

Cited by 86 publications

(64 citation statements)

References 13 publications

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“…However, such substances in PPE are likely to be small molecules including tripeptides and dipeptides, because PPE has shown bioactivity via oral administration for the treatment of wrinkles, climacteric symptoms, and knee pain [13,14]. Collagen is characterized by a triple helical structure with a repeating sequence of Glycine (G)-X-Y, where amino acids X and Y are high-frequency proline and hydroxyl proline, respectively [32]. Togashi et al, reported that HPE contains G-X-Y tripeptides [32].…”

Section: Discussionmentioning

confidence: 99%

“…Collagen is characterized by a triple helical structure with a repeating sequence of Glycine (G)-X-Y, where amino acids X and Y are high-frequency proline and hydroxyl proline, respectively [32]. Togashi et al, reported that HPE contains G-X-Y tripeptides [32]. The G-X-Y tripeptide reportedly increases collagen and hyaluronic acid contents in cultured fibroblasts [33].…”

Section: Discussionmentioning

confidence: 99%

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Effect of Porcine Placental Extract on Collagen Production in Human Skin Fibroblasts In Vitro

Yoshikawa

Takano²,

Ishigaki³

et al. 2013

Gynecol Obstet (Sunnyvale)

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Placental Extract (PE) has been used as a folk remedy in Asian countries. Human PE is clinically used for the treatment of skin wrinkles because it promotes skin regeneration. In this study, we found that Porcine PE (PPE) promoted collagen production in Normal Human Dermal Fibroblasts (NHDFs). Treatment of NHDFs with PPE at concentrations of 12.5-100 μg/mL enhanced their proliferation and secretion of procollagen C peptide. We also found that expression of intracellular Matrix Metalloproteinase-9 (MMP-9) in PPE-treated NHDFs was decreased in a dose-dependent manner. These results indicate that PPE might affect facial wrinkling partly by enhancing fibroblast proliferation and collagen secretion while inhibiting MMP-9 expression in NHDFs.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Effect of Porcine Placental Extract on Collagen Production in Human Skin Fibroblasts In Vitro

Yoshikawa

Takano²,

Ishigaki³

et al. 2013

Gynecol Obstet (Sunnyvale)

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“…Thus HPEs have been shown to scavenge hydroxyl radical, nitric oxide, and superoxide radical; to reduce ferric iron; to chelate transition metal ions; and to prevent lipid peroxidation [6,7]. Such activity may be explained by the presence of antioxidants able to be donors of electrons such as phenol compounds, ascorbic acid, uric acid, some SH-group-containing compounds and protein amino acids [8]. These facts support HPE application in clinical practice in order to treat oxidation-induced diseases including atherosclerosis, aging, inflammation, and certain nervous system disorders.…”

Section: Introductionmentioning

confidence: 99%

Protective effect of placenta extracts against nitrite-induced oxidative stress in human erythrocytes

Rozanova

Cherkashina

Repina

et al. 2012

Cellular and Molecular Biology Letters

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Aqueous-saline human placenta extract (HPE) is known to possess antioxidant activity due to the high concentration of bioactive substances. This fact allows its application in clinical practice in order to treat oxidation-induced diseases. Extract antioxidant activity is mainly conditioned by proteins. Freezing of extracts has been shown to lead to their antioxidant activity increasing due to protein conformation changes. Different biological models are widely used in order to evaluate efficacy of novel antioxidants and mechanisms of their action. One such model appears to be erythrocytes under nitrite-induced oxidative stress. Nitrite is known to be able to penetrate erythrocyte membrane and to oxidize hemoglobin. In order to investigate whether HPE is able to decrease nitrite-induced oxidative injuries and to evaluate the protein contribution to this process, spectrophotometric and electron spin resonance (ESR) assays were used. Experimental data have revealed that antioxidant activity of extracts and of some of their fractions correlates with methemoglobin concentration lowering. Preliminary erythrocyte incubation with an extract fraction of 12 kDa allows preservation of the structural-dynamic cytosol state the closest to the control.

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“…The nutritional substances and vitamins therein, known as the placental extract (PE), can be extracted. The placenta is considered a reservoir of cytokines, hormones, bioactive peptides, enzymes, growth factors, vitamins and minerals (Togashi et al, 2002). PE also contains many valuable bioactive compounds that have various bio-capabilities, including inhibition of aging, inflammation, sunburn, gene mutation, anaphylacticity and oxidation (Kim et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

Immune Modulation Effect of Pig Placenta Extracts in a Mouse Model: Putative Use as a Functional Food Supplement

Park¹,

Suh²,

Kim³

et al. 2011

Korean Journal for Food Science of Animal Resources

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This study was performed to establish an effective extraction method of pig placenta extract that could be used for a putative functional food supplement with immunomodulatory effects. In the present study, we used different temperatures (4, 37, 60, 80, and 100 o C) and different solvents (chloroform, NaOH, and phosphate buffered saline [PBS]) to extract the pig placenta. Among the different placenta extracts yielded by the different extraction methods, placenta extract (PE) in PBS at 80 o C for 30 min (referred to as PE-PBS80) showed a significant increase of nitric oxide production of up to 22.97 µM/10 5 cells at a 1 mg/mL dose (p<0.05 ) in J774A.1 cells than other extracts and control tested. Using PE-PBS80, further animal challenges were performed to identify the immune-enhanced effects. As a result, orally administered PE-PBS80 showed a significant increase in blood T and B cell activities and immunoglobulin (IgG and IgM) production. IgG and IgM levels increased to 41.53 mg/mL at a 20 mg dose on day 7 and to 27.38 mg/mL at a 10 mg dose on day 14, respectively (p<0.05). Furthermore, PE-PBS80 was also able to significantly enhance the immune modulator cytokine levels (p<0.05) compared to the control and vehicle treatments. Among the evaluated cytokines, the tumor necrosis factor-α (TNF-α) level increased to 28.89 pg/mL at extract doses of 20 and 50 mg, the interleukin-1β (IL-1β) level increased to 21.52 pg/mL at extract doses of 10, 20, 50 and 75 mg and the interferon (IFN)-γ level increased to 18.24 pg/mL at extract doses of 10, 20, and 50 mg. Therefore, this study presents an effective method for extracting pig placenta extracts and also demonstrates that pig placenta extracts had significant immunomodulatory effects not only at the cellular level but also in a mouse model, suggesting that this material could be used as an excellent candidate functional food supplement.

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Antioxidative Collagen-Derived Peptides In Human-Placenta Extract

Cited by 86 publications

References 13 publications

Effect of Porcine Placental Extract on Collagen Production in Human Skin Fibroblasts In Vitro

Effect of Porcine Placental Extract on Collagen Production in Human Skin Fibroblasts In Vitro

Protective effect of placenta extracts against nitrite-induced oxidative stress in human erythrocytes

Immune Modulation Effect of Pig Placenta Extracts in a Mouse Model: Putative Use as a Functional Food Supplement

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