Antioxidant therapy enhances pulpal healing in bleached teeth

Abstract

ObjectivesThe purpose of this study was to evaluate the histopathological effects of an antioxidant therapy on the pulp tissue of rat teeth exposed to a bleaching gel with 35% hydrogen peroxide.Materials and MethodsForty rats were subjected to oral ingestion by gavage of distilled water (DW) or ascorbic acid (AA) 90 min before the bleaching therapy. For the bleaching treatment, the agent was applied twice for 5 min each to buccal surfaces of the first right mandibular molars. Then, the animals were sacrificed at 6 hr, 24 hr, 3 day, or 7 day post-bleaching, and the teeth were processed for microscopic evaluation of the pulp tissue.ResultsAt 6 hr, the pulp tissue showed moderate inflammatory reactions in all teeth of both groups. In the DW and AA groups, 100% and 80% of teeth exhibited pulp tissue with significant necrosis and intense tissue disorganization, respectively. At 24 hr, the AA-treated group demonstrated a greater regenerative capability than the DW group, with less intense inflammatory reaction and new odontoblast layer formation in 60% of the teeth. For up to the 7 day period, the areas of pulpal necrosis were replaced by viable connective tissue, and the dentin was underlined by differentiated odontoblast-like cells in most teeth of both groups.ConclusionsA slight reduction in initial pulpal damage during post-bleaching was promoted by AA therapy. However, the pulp tissue of AA-treated animals featured faster regenerative potential over time.

Concentration‐dependent effect of bleaching agents on the immunolabelling of interleukin‐6, interleukin‐17 and CD5‐positive cells in the dental pulp

“…Furthermore, a recent study revealed areas of necrosis after 6 h of application of bleaching gel (Lima et al 2016). Thus, it may be that the pulp tissue observed in the present study at 2 days is in the process of regeneration.…”

Human Dental Pulp Stem Cells Exhibit Different Biological Behaviours in Response to Commercial Bleaching Products

“…HP reaches the pulp chamber via the dentinal tubules, decreases cell metabolism and viability [ 6 , 7 ] and induces vascular permeability changes [ 8 ], DNA modifications and pulpal necrosis [ 9 , 10 , 11 , 12 ].…”

Influence of dental bleaching on the pulp tissue: A systematic review of in vivo studies

“…The inflammatory response in pulp tissues was evaluated in 27 in vivo studies, primarily assessed based on the intensity of inflammatory infiltrate in different thirds of pulp tissue (Benetti et al., 2017; Benetti, Briso, et al., 2018; Benetti, Briso, Carminatti, et al., 2019; Benetti, Briso, de Araújo Lopes, et al., 2019; Benetti, Gomes‐Filho, et al., 2018; Carminatti et al., 2020; Cintra et al., 2013; Cintra et al., 2017; Cintra, Benetti, Ferreira, Gomes‐Filho, et al., 2016; Costa et al., 2010; da Silva et al., 2020; Ferreira et al., 2018; Gallinari et al., 2019; Kina et al., 2010; Lima et al., 2016; Louzada et al., 2019; Robertson & Melfi, 1980; Roderjan et al., 2015; Silva‐Costa et al., 2018; Terayama et al., 2020; Vaz et al., 2016), or by counting inflammatory cells (Cintra, Benetti, Ferreira, Rahal, et al., 2016).…”

“…In animal studies (19 studies), 18 of them assessed the inflammatory response, and all demonstrated significant inflammation in the most superficial regions of coronal pulp, often accompanied by necrotic areas. This was particularly evident after bleaching with 35% or 38% HP and during the initial periods (Barbosa et al., 2019; Benetti et al., 2017; Benetti, Briso, et al., 2018; Benetti, Briso, Carminatti, et al., 2019; Benetti, Briso, de Araújo Lopes, et al., 2019; Benetti, Gomes‐Filho, et al., 2018; Carminatti et al., 2020; Cintra et al., 2013, 2017; Cintra, Benetti, Ferreira, Gomes‐Filho, et al., 2016; da Silva et al., 2020; Ferreira et al., 2018; Gallinari et al., 2019; Lima et al., 2016; Louzada et al., 2019; Seale et al., 1981; Silva‐Costa et al., 2018; Terayama et al., 2020).…”