Antioxidant systems and erythrocyte life-span in mammals

Kurata, Masahiro; Suzuki, Masatoshi; Agar, Nicholas

doi:10.1016/0305-0491(93)90121-k

Cited by 185 publications

(120 citation statements)

References 94 publications

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“…Erythrocytes are particularly susceptible to oxidative damage because (i) being oxygen carriers, they are exposed uninterruptedly to high oxygen tension [33,34] and (ii) hemoglobin is susceptible to autoxidation and their membrane components to lipid peroxidation [35,36]. L-carnitine did not play a major role in maintaining Hb.…”

Section: Discussionmentioning

confidence: 95%

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l-carnitine as a Potential Additive in Blood Storage Solutions: A Study on Erythrocytes

Ravikumar

Hsieh

Vani

2015

Indian J Hematol Blood Transfus

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Erythrocytes undergo various changes during storage (storage lesion) that in turn reduces their functioning and survival. Oxidative stress plays a major role in the storage lesion and antioxidants can be used to combat this stress. This study elucidates the effects of L-carnitine (LC) on erythrocytes of stored blood. Blood was obtained from male Wistar rats and stored (4°C) for 20 days in CPDA-1 (citrate phosphate dextrose adenine) solution. Samples were divided into-(i) controls (ii) LC 10 (L-carnitine at a concentration of 10 mM) (iii) LC 30 (L-carnitine at a concentration of 30 mM) and (iv) LC 60 (L-carnitine at a concentration of 60 mM). Every fifth day, the biomarkers (haemoglobin, hemolysis, antioxidant enzymes, lipid peroxidation and protein oxidation products) were analysed in erythrocytes. Hemoglobin and protein sulfhydryls were insignificant during storage indicative of the maintenance of hemoglobin and sulfhydryls in all groups. Superoxide dismutase and malondialdehyde levels increased initially and decreased towards the end of storage. The levels of catalase and glutathione peroxidase were lower in experimentals than controls during storage. L-carnitine assisted the enzymes by scavenging the reactive oxygen species produced. Hemolysis increased in all groups with storage, elucidating that L-carnitine could not completely protect lipids and proteins from oxidative stress. Hence, this study opens up new avenues of using L-carnitine as a component of storage solutions with combinations of antioxidants in order to maintain efficacy of erythrocytes.

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Section: Discussionmentioning

confidence: 95%

“…Catalase and GSH-Px act on the same substrate, H 2 O 2 , at high and low concentrations respectively [35,45,46]. In accordance with the SOD activity, CAT and GSH-Px were activated initially during storage.…”

Section: Discussionmentioning

confidence: 99%

l-carnitine as a Potential Additive in Blood Storage Solutions: A Study on Erythrocytes

Ravikumar

Hsieh

Vani

2015

Indian J Hematol Blood Transfus

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“…SOD and GSH are two antioxidant substances widely distributed in the organism, so they are measured as a reference for the damage degree of tissue lipid peroxidation. [48,49] GSH helps in detoxification and excretion of toxic compounds. [50] GSH directly interacts ROS or is involved in enzymatic detoxification reactions for ROS as a co-factor.…”

Section: Discussionmentioning

confidence: 99%

Nigella sativa protects against formaldehyde-induced neurotoxicity in the rat frontal cortex

Mohamad¹,

Essa²,

Ata³

2015

Anatomy

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Formaldehyde (FA) is a compound commonly used in industry and medicine that can react with different macromolecules such as proteins, nucleic acids and low molecular weight substances as amino acids. [1,2] In particular, anatomists and medical students are exposed to FA gas during cadaver dissections. [3,4] Humans are exposed to FA from direct environmental sources like paint, plywood, cosmetics, cigarette smoke, photochemical smog and even various fruits as well as from the metabolism of xenobiotics. [5,6] FA is accepted as toxic over certain doses and its at high room temperatures its harmful effects increase due to volatility. [7][8][9][10] Physiological FA forms by the metabolism of L-methionine, histamine or methylamine in the body, and contribute to biological methylation by folic acid. [11] Acute exposure to FA causes central nervous system symptoms such as headache, malaise, insomnia, anorexia

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“…Previous investigations of SOD activity in RBC have shown that it is quite uniform among a variety of animal species, ranging from 551 ± 236 EU/g Hb in the grey-headed fruit bat to 3058 ± 542 EU/g Hb in cattle (Maral et at., 1977;Suzuki, 1982;Wightman et al, 1986;Agar et at., 1989;Kurata et at., 1993a). The results of the present investigation fall within this range.…”

Section: Superoxide Dismutase (Sod)mentioning

confidence: 99%

Comparative study of the antioxidant defence systems in the erythrocytes of australian marsupials and monotremes

Whittington

Parkinson

Spencer

et al. 1995

Comparative Biochemistry and Physiology Part C: Pharmacology, T

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A comparison of the erythrocyte (RBC) antioxidant metabolites and enzymes in nine marsupial and two monotreme species was carried out. Reduced glutathione (GSH) concentrations were comparable with those reported for other marsupial and eutherian species. An important finding was that the erythrocytes of the southern hairy nosed wombat regenerated GSH faster than the erythrocytes from its close relative, the common wombat. The activities of glutathione-S-transferase, NADH-methaemoglobin reductase, superoxide dismutase, and glutathione peroxidase (GSH-Px), showed similar levels and extents of variation as those observed in other marsupial and eutherian species. Catalase activities in the marsupials were lower than those measured in the two monotreme species and much lower than those reported in eutherian species. A negative correlation, significant at P < 0.05, was observed between GSH-Px and catalase activities in the RBC of the marsupials. Since both these enzymes "detoxify" H 2 0 2 , there appears to be a reciprocal relationship between the activities of these enzymes in marsupial RBC.

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Antioxidant systems and erythrocyte life-span in mammals

Cited by 185 publications

References 94 publications

l-carnitine as a Potential Additive in Blood Storage Solutions: A Study on Erythrocytes

l-carnitine as a Potential Additive in Blood Storage Solutions: A Study on Erythrocytes

Nigella sativa protects against formaldehyde-induced neurotoxicity in the rat frontal cortex

Comparative study of the antioxidant defence systems in the erythrocytes of australian marsupials and monotremes

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