Antioxidant Stability Testing On Liquid And Powder Eichhornia Crassipes Extract

Abstract

Water hyacinth or Eicchornia crassipes is a free-floating aquatic plant and is easily found in swamps or rivers. The rapid growth of water hyacinth can disrupt the aquatic environment, thus water hyacinth is categorized as one of the world’s worst weed. The challenge is not on how to eliminate the number of this weed, but on how to take advantage by the presence of this water plant. Water hyacinth is known to contain high phenolic content and antioxidant activity. Water hyacinth was extracted using 96% ethanol/water with mass to solvent ratio of 1:30 at 3 different temperatures (30°C, 40°C, 50°C). Spray drying was conducted to produce extract powder with the addition of maltodexrin and Arabic gum as the encapsulating agents. The stability of liquid and powder extract was compared based on the Total Phenolic Content (TPC) and antioxidant activity. The highest TPC was obtained from extraction at 30°C whereas the highest antioxidant activity was obtained from extraction at 50°C. In powder extract, the highest TPC and antioxidant activity was obtained from powder with ratio 2:1 of TSS to encapsulating agents. The stability was performed under different storage conditions. The degradation of TPC was faster in room temperature.

Antioxidant and Cytotoxic Effects of the Methanolic Extract of Eichhornia crassipes Petioles Upon Mg-63 Cell Lines: An In Vitro Study

“…Tyagi and Agarwal determined the antioxidant potential, total polyphenols, and flavonoids of E. crassipes from methanolic extracts of various sections of plants and demonstrated their therapeutic and pharmaceutical values [ 29 ]. According to Nugriani et al, the powdered preparation of E. crassipes displayed higher TPC and antioxidant properties with a proportion of 1:2 than the various liquid extracts [ 30 ].…”

Manufacturing Process Development of Health Supplement Containing Water Hyacinth (Eichhornia crassipes) Extract

“…The powder extract samples used in the toxicity analysis at IMERI Laboratory were variated based on the extraction temperature of WH leaves and storage temperature. In order to produce the powder extract, the liquid extract were spray-dried, using encapsulating agents (equal mixture of maltodextrin and Arabic gum) with mass ratio of 1:1 of the total soluble solids (TSS) to total mass of encapsulating agents [3].…”

“…The extraction process to extract the antioxidant content from WH powder will use a mixture of 96% ethanol and water in a 1:1 ratio by weight [3] at a temperature of 50℃ with constant stirring for 3 hours. The extracted solution will then be dried by means of spray drying to remove the remaining solvent.…”

“…Both processes might not be efficient for maintaining TPC, but the processes are necessary for the manufacturing process and to remove the rest of the solvent from the extract, so that safety of the product can be ensured. Each tablet contains 100 mg of WH extract which proven to have an antioxidant activity of 6.3071 mg GAE, using the assumption that the TPC of the powder extract is 63.07 mg GAE/g powder while the IC50 of the powder extract shows the concentration of 306.75 ppm [3].…”

“…In the leaves, the ethanolic extract showed good 2,2-Diphenyl-1-Picrylhydrazyl hydrate (DPPH) radical scavenging activities, with an IC50 of 55.76 ± 6.73 ppm [2]. A further study performed a stability testing on the antioxidant activity of water hyacinth leaves powder extract and showed a shelf life of 35.51 days under refrigeration [2,3].…”