Antioxidant responses and reactive oxygen species generation in different body regions of the estuarine polychaeta Laeonereis acuta (Nereididae)

Ferreira-Cravo, Marlize; Piedras, Fernanda Reinhardt; Moraes, Tarsila Barros; Ferreira, Josencler L. Ribas; Freitas, Diana Paula Salomão de; Machado, Márcia Dornelles; Geracitano, Laura A.; Monserrat, José M.

doi:10.1016/j.chemosphere.2006.06.050

Cited by 78 publications

(25 citation statements)

References 20 publications

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“…This place is considered to be free from anthropogenic inputs and presents low metal content in the sediment. Besides, worms collected at this site presented lower antioxidant responses when compared with worms sampled at sites with prevailing pro-oxidant conditions (i.e., high levels of copper) (Ferreira-Cravo et al 2007). The organisms were transferred to the laboratory in ice-cooled saline water (10‰), where they were transferred to glass dishes (6.0 cm diameter; one in each dish) with sand and water at 10‰ (pH 8.00; 20°C) for 6 days (Geracitano et al 2004).…”

Section: Worm Collection Maintenance and Exposure Designmentioning

confidence: 99%

“…Immediately before the reading, 10 μl of the fluorescent probe 2′,7′ dichlorofluorescein diacetate (H 2 DCF-DA) was added to each well at a final concentration of 40 μM (Ferreira-Cravo et al 2007). H 2 DCF-DA is cleaved by esterases present in the samples and the non-fluorescent compound H 2 DCF is oxidized by peroxyl radicals to the fluorescent compound DCF, which is detected at wavelengths of 485 and 530 nm, for excitation and emission, respectively.…”

Section: Determination Of Antioxidant Capacity Against Peroxyl Radicalsmentioning

confidence: 99%

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Accumulation, biotransformation, and biochemical responses after exposure to arsenite and arsenate in the estuarine polychaete Laeonereis acuta (Nereididae)

Ventura‐Lima

Ramos

Fattorini

et al. 2011

Environ Sci Pollut Res

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Section: Worm Collection Maintenance and Exposure Designmentioning

confidence: 99%

Section: Determination Of Antioxidant Capacity Against Peroxyl Radicalsmentioning

confidence: 99%

Accumulation, biotransformation, and biochemical responses after exposure to arsenite and arsenate in the estuarine polychaete Laeonereis acuta (Nereididae)

Ventura‐Lima

Ramos

Fattorini

et al. 2011

Environ Sci Pollut Res

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“…Numerous environmental variables have the capacity to trigger the antioxidant defence system in polychaete species, such as solar radiance, hydrogen peroxide, salinity, water temperature, oxygen, and also exposure to several classes of contaminants , Ait Alla et al 2006, Moreira et al 2006, Ferreira-Cravo et al 2007, Sun and Zhou 2008, Douhri and Sayah 2009.…”

Section: Discussionmentioning

confidence: 99%

Biomarkers in <i>Nereis diversicolor</i> (Polychaeta: Nereididae) as management tools for environmental assessment on the southwest Iberian coast

et al. 2013

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SUMMARY:The environmental quality of the southwest Iberian coast was assessed in different areas (Ria Formosa Lagoon, Guadiana Estuary and Cádiz Bay) focusing on metal contamination (Cd, Cu, Ni, Pb and Zn) in Nereis diversicolor whole tissues. In addition, associated toxicological effects were assessed using a multibiomarker approach combining several conventional biomarkers. The set of biomarkers selected included antioxidant enzymes (catalase, superoxide dismutase and glutathione peroxidase), metallothionein and lipid peroxidation. N. diversicolor from the Ria Formosa Lagoon, Guadiana River and Cádiz Bay responded differently to metal contamination with different biomarker responses showing a clear site trend, suggesting different sources and/or magnitudes of contamination. Cadmium was a source of oxidative stress in polychaetes, mainly in Cádiz Bay, with a significant influence on antioxidant enzymes and enhancement of lipid peroxidation. The highest MT concentrations were in the Ria Formosa Lagoon and in the Guadiana River, where there was a direct relationship with high nickel concentrations. Biomarker responses of N. diversicolor are useful tools for environmental quality assessment on the southwest Iberian coast, and more specific metal biomarkers should be included in future assessments.Keywords: biomarkers, metals, Nereis diversicolor, antioxidant enzymes, metallothionein, lipid peroxidation, southwest Iberian coast.RESUMEN: Biomarcadores en Nereis diversicolor (Polychaeta: nereididae) como herramientas de gestión de la evaluación del medio amBiente en el suroeste de la Península iBérica. -El estudio de la calidad ambiental de los ecosistemas costeros del SO de la Península Ibérica (Ría Formosa, estuario del Guadiana y bahía de Cádiz) en relación a la contaminación metálica (Cd, Cu, Ni, Pb y Zn) se ha llevado a cabo mediante el análisis del contenido de metales en Nereis diversicolor. Además, los efectos ecotoxicológicos fueron evaluados mediante una aproximación "multibiomarcadores", en donde se combinan varios biomarcadores convencionales. La batería de biomarcadores seleccionadas han sido: las enzimas antioxidantes (catalasa (CAT), superóxido dismutasa (SOD) y glutatión peroxidasa (GPX), metalotioneína (MT) y peroxidación lipídica (LPO). Los ejemplares de N. diversicolor recolectados en las distintas zonas, mostraron una respuesta diferente a la contaminación metálica, lo que sugiere diferentes fuentes y/o magnitud de la contaminación. Los niveles de cadmio estan directamente relacionados con las actividades SOD y CAT y los niveles de LPO. En el caso del níquel, sus niveles se hallaban correlacionados positivamente con los de MT en la Ría Formosa y en el río Guadiana. La integración de las respuesta de los biomarcadores en N. diversicolor ha demostrado ser una herramienta útil para la evaluación de la calidad ambiental de los ecosistemas costeros del SO de la Península Ibérica.

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“…ROS oxidizes 2,7-dichlorofluorescin diacetate, generating a DCF fluorescent compound (2,7-dichlorofluorescein), which is used as an indirect marker of oxidant stress. 32 DCF is detectable by flow cytometry at wavelengths of 488 (excitation) and 525 nm (emission). Immediately before the microplate reading, 10 ml of the fluorescent probe 2,7-dichlorofluorescin diacetate, at a final concentration of 40 mM, was added to wells containing the cell suspension.…”

Section: Generation Of Ros In Cd34 + Cellsmentioning

confidence: 99%

Circulating progenitor cells are increased in newly diagnosed untreated hypertensive patients with arterial stiffening but normal carotid intima-media thickness

et al. 2011

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Circulating progenitor cells (CPCs), including endothelial progenitor cells (EPCs), have a key role in endothelium repair. Cellular NADPH oxidase (Nox) enzymes, including Nox-containing gp91phox, represent a source of reactive oxygen species (ROS); ROS trigger protective signals but may also have detrimental effects. Cellular defenses against ROS include the enzymes manganese superoxide dismutase (MnSOD), catalase (CAT) and glutathione peroxidase type-1 (GPx-1). We investigated the relationships of CPCs with cellular gp91phox, MnSOD, CAT, GPx-1 and ROS levels and with carotid intima-media thickness (cIMT) and stiffness in hypertensives without additional risk factors for cardiovascular disease. CPCs from 53 newly diagnosed, untreated hypertensives and from 29 controls were isolated and identified by flow cytometry. gp91phox, MnSOD, CAT, and GPx-1 mRNA and protein expression and ROS generation were evaluated in enriched samples of CD34 + cells; cIMT and stiffness were assessed. Hypertensives showed higher arterial stiffness (Po0.001) but no difference in cIMT with respect to controls. ROS generation was slightly increased (P¼0.04), whereas gp91phox, MnSOD, CAT and GPx-1 were significantly higher (Po0.001) with respect to controls, as was CPC number (Po0.001), but EPCs were no different. CPC and EPC numbers correlated with gp91phox, ROS and fibrinogen (Po0.001); moreover, gp91phox, MnSOD, CAT and GPx-1 were correlated with CPC number. In early phases of arterial hypertension, before the development of wall thickening and even in the presence of arterial mechanical impairment, CPC number may be increased to maintain an adequate number of EPCs in peripheral blood.

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Antioxidant responses and reactive oxygen species generation in different body regions of the estuarine polychaeta Laeonereis acuta (Nereididae)

Cited by 78 publications

References 20 publications

Accumulation, biotransformation, and biochemical responses after exposure to arsenite and arsenate in the estuarine polychaete Laeonereis acuta (Nereididae)

Accumulation, biotransformation, and biochemical responses after exposure to arsenite and arsenate in the estuarine polychaete Laeonereis acuta (Nereididae)

Biomarkers in <i>Nereis diversicolor</i> (Polychaeta: Nereididae) as management tools for environmental assessment on the southwest Iberian coast

Circulating progenitor cells are increased in newly diagnosed untreated hypertensive patients with arterial stiffening but normal carotid intima-media thickness

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