Antioxidant properties of the mucus secreted by Laeonereis acuta (Polychaeta, Nereididae): A defense against environmental pro-oxidants?

Moraes, Tarsila Barros; Ferreira, Josencler L. Ribas; Rosa, Carlos Eduardo da; Sandrini, Juliana Zomer; Votto, Ana Paula S.; Trindade, Gilma Santos; Geracitano, Laura A.; Abreu, Paulo César; Monserrat, José M.

doi:10.1016/j.cbpc.2005.10.017

Cited by 22 publications

(23 citation statements)

References 38 publications

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“…Moraes et al (2006) showed that the mucus secretion of the polychaete Laeonereis acuta is colonized by bacteria communities that confer antioxidant properties. In the present study, the results indicated altered antioxidant responses and ameliorated lipid peroxidation in shrimps maintained in the BFT system.…”

Section: Discussionmentioning

confidence: 99%

Antioxidant and oxidative damage responses in different organs of Pacific white shrimpLitopenaeus vannamei(Boone, 1931) reared in a biofloc technology system

Martins

Flores

Wasielesky

et al. 2015

Marine and Freshwater Behaviour and Physiology

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Shrimp (Litopenaeus vannamei) reared in a conventional seawater (SW) aquarium system SW were compared with those raised in a biofloc technology (BFT) system. After 30 days, the L. vannamei shrimp were euthanized and samples of gills, hepatopancreas and muscle were dissected. Statistical analysis was performed using bi-factorial ANOVA, with the factors of the treatment (shrimp reared in SW or in a BFT system) and organs (gills, hepatopancreas and muscles). No differences (p > 0.05) in glutathione-S-transferase activity were observed between shrimp reared in SW and shrimp reared in BFT (p < 0.05). Glutathione levels were lower (p < 0.05) in the gills and hepatopancreas of shrimp reared in the BFT system, suggesting changes in the antioxidant composition of these organs. Lipid peroxidation levels were higher in the hepatopancreas than in muscle in shrimp reared in SW (p < 0.05) and this difference was not observed in organisms reared in the BFT system (p > 0.05). Overall, the results showed that rearing shrimp in the BFT system altered both their antioxidant and oxidative damage responses. This indicates that some microbial communities in BFT systems can influence the redox state of L. vannamei.

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Section: Discussionmentioning

confidence: 99%

Antioxidant and oxidative damage responses in different organs of Pacific white shrimpLitopenaeus vannamei(Boone, 1931) reared in a biofloc technology system

Martins

Flores

Wasielesky

et al. 2015

Marine and Freshwater Behaviour and Physiology

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“…Enzymatic antioxidants such as peroxiredoxin, catalase and superoxide dismutase have already been described in various human and invertebrate mucus (see supplemental Table S4). For instance, such enzymes are found in the mucus secreted by the polychaete Laeonereis acuta and act as an antioxidant defence system by intercepting and degrading ROS [58]. Non-enzymatic antioxidants such as ferritin and melanotransferrin were also highlighted in sea star mucus.…”

Section: Accepted Manuscriptmentioning

confidence: 99%

An integrated transcriptomic and proteomic analysis of sea star epidermal secretions identifies proteins involved in defense and adhesion

Hennebert

Leroy

Wattiez

et al. 2015

Journal of Proteomics

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Sea stars rely on epidermal secretions to cope with their benthic life. Their integument produces a mucus, which represents the first barrier against invaders; and their tube feet produce adhesive secretions to pry open mussels and attach strongly but temporarily to rocks. In this study, we combined high-throughput sequencing of expressed mRNA and mass-spectrometry-based identification of proteins to establish the first proteome of mucous and adhesive secretions from the sea star Asterias rubens. We show that the two secretions differ significantly, the major adhesive proteins being only present in trace amounts in the mucus secretion. Except for 41 proteins which were present in both secretions, a total of 34 and 244 proteins were identified as specific of adhesive secretions and mucus, respectively. We discuss the role of some of these proteins in the adhesion of sea stars as well as in their protection against oxygen reactive species and microorganisms. In addition, 58% of the proteins identified in adhesive secretions did not present significant similarity to other known proteins, revealing a list of potential novel sea star adhesive proteins uncharacterized so far. The panel of proteins identified in this study offers unprecedented opportunities for the development of sea star-inspired biomimetic materials.

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“…Such gentle centrifugation is not intense enough to pellet down proteins (which requires ultracentrifugation) but can pellet down structures (lamellar bodies, lattices) formed from cross-linked glycosylated proteins such as mucins10. Such gentle centrifugation is in fact used to separate active compounds from the mucin network1112, the active compounds then being “squeezed out” of the mucus and found in the less-viscous, and easier-to-work-with, supernatant. Here, the highly viscous phase (pellet) behaved like the native mucus in terms of bioluminescence, producing spontaneous light that was inhibited by H 2 O 2 (Fig.…”

Section: Resultsmentioning

confidence: 99%

Evidence that ferritin is associated with light production in the mucus of the marine worm Chaetopterus

Rawat

Deheyn

2016

Sci Rep

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The blue glow of the mucus from Chaetopterus involves a photoprotein, iron and flavins. Identity and respective role of these components remain, however, largely unresolved today, likely because of viscosity issues and inhibition of this system by oxidizers conventionally used to track bioluminescence activity. Here, we used gentle centrifugation to obtain a mucus supernatant showing no inhibition to oxidizers, allowing for further analysis. We applied conventional chromatographic techniques to isolate major proteins associated with light emission. Luminescence ability of elutriate fractions was tested with hydrogen peroxide to track photoprotein and/or protein-bound chromophore. Fractions producing light contained few major proteins, one with similarity to ferritin. Addition to the mucus of elements with inhibitory/potentiary effect on ferritin ferroxidase activity induced corresponding changes in light production, emphasizing the possible role of ferritin in the worm bioluminescence. DNA of the protein was cloned, sequenced, and expressed, confirming its identity to a Chaetopterus Ferritin (ChF). Both ferric and ferrous iron were found in the mucus, indicating the occurrence of both oxidase and reductase activity. Biochemical analysis showed ChF has strong ferroxidase activity, which could be a source of biological iron and catalytic energy for the worm bioluminescence when coupled to a reduction process with flavins.

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Antioxidant properties of the mucus secreted by Laeonereis acuta (Polychaeta, Nereididae): A defense against environmental pro-oxidants?

Cited by 22 publications

References 38 publications

Antioxidant and oxidative damage responses in different organs of Pacific white shrimpLitopenaeus vannamei(Boone, 1931) reared in a biofloc technology system

Antioxidant and oxidative damage responses in different organs of Pacific white shrimpLitopenaeus vannamei(Boone, 1931) reared in a biofloc technology system

An integrated transcriptomic and proteomic analysis of sea star epidermal secretions identifies proteins involved in defense and adhesion

Evidence that ferritin is associated with light production in the mucus of the marine worm Chaetopterus

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