Antioxidant isoenzyme responses to nickel-induced stress in tobacco cell suspension culture

Pompeu, Geórgia Bertoni; Gratão, Priscila Lupino; Vitorello, Victor Alexandre; Azevedo, Ricardo Antunes

doi:10.1590/s0103-90162008000500015

Cited by 33 publications

(29 citation statements)

References 19 publications

(21 reference statements)

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“…In any case, a higher protection against oxidative damage may require a rapid metabolism of H 2 O 2 generated by the action of SOD. Therefore, for the proper functioning of the detoxification of free radicals, subsequent antioxidant enzymes in the system, such as catalase (CAT) and the ascorbate peroxidase (APX), may work in synchrony to remove H 2 O 2 (Gratão et al, 2005;Pompeu et al, 2008). In this case, CAT ( Figure 4B) and APX ( Figure 4C) had similar behavior to SOD for plants under both stressful conditions.…”

Section: Resultsmentioning

confidence: 93%

“…The H 2 O 2 production occurs in the plants tissues under a variety of factors that include extreme temperatures, excessive excitement energy, water stress, heavy metal and pathogen action (Deuner et al, 2008;GomesJúnior et al, 2006;Pompeu et al, 2008). The H 2 O 2 production may occur in plant cells via a number of patways, such as NAD(P)H oxidase, peroxidation of lipids or by the transport of electrons in photosynthesis (Montillet et al, 2004).…”

Section: Resultsmentioning

confidence: 99%

“…Among the various enzymes involved in the elimination of ROS, SOD can be considered a key enzyme and is usually the first line of defense mechanism against oxidative stress (Gratão et al, 2005;Pompeu et al, 2008). Water deficit induced a higher activity of SOD, which determines the concentration of O 2 · -and H 2 O 2 , being central in the defense mechanisms required to prevent the formation of OH · radical (Yoshimura et al, 2000).…”

Section: Resultsmentioning

confidence: 99%

“…To protect its cells from ROS, drought tolerant plants activate an oxidative defense system, formed by enzymatic and non-enzymatic components capable of maintaining a favorable balance between ROS and the detoxifying capacity (Pompeu et al, 2008;Vasconcelos et al, 2009). Moreover, plant responses to different types of stresses are associated with generation of ROS, suggesting that ROS may function as a common signal in signaling pathways of plant stress responses (Gratão et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

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Stomatal behavior and components of the antioxidative system in coffee plants under water stress

Deuner¹,

Alves²,

Zanandrea

et al. 2011

Sci. agric. (Piracicaba, Braz.)

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Coffee (Coffea arabica) plants show a positive relationship between stomatal closure and formation and accumulation of H 2 O 2 . However, for coffee plants under water restriction such relationship has never been studied. The objective of the present study was evaluate the stomatal movement and the antioxidant capacity of coffee seedlings under different water regimes. Eight months old coffee seedlings of cv. Catuaí IAC 99 were submitted to field capacity, gradual and total suspension of irrigation during a period of 21 days. Evaluations of leaf water potential (Ψ w ) were performed in the beginning of the morning, and stomatal resistance, transpiration rate and vapor pressure deficit were determined at 10 am and 5 pm. All biochemical and enzymatic determinations were performed in leaves collected at 5 pm. Evaluations and samplings were performed at three days intervals. There was no variation in Ψ w during the evaluated period for plants in field capacity. However, an expressive decrease of Ψ w following day 12, reaching values near -2.5 MPa at the end of the experiment was observed for plants submitted to gradual suspension of irrigation. For plants submitted to total suspension of irrigation, Ψ w decreases after the sixth day, reaching -2.5 MPa at day 15. The decay of Ψ w in plants submitted to gradual and total suspension of irrigation reflected in increased stomatal resistance and in a decreased transpiration rate leading to an increase in hydrogen peroxide formation and, on final stages, increase in lipid peroxidation. As a conclusion, an increase in the activity of antioxidant enzymes as well as in the levels of ascorbate and dehydroascorbate was observed, which act in the detoxification of free radicals formed as result of the water stress. Key words: Coffea arabica, oxidative stress, antioxidants, water stressComportamento estomático e componentes do sistema antioxidante em cafeeiros sob estresse hídrico RESUMO: Para o cafeeiro (Coffea arabica) existe uma comprovada relação positiva entre fechamento estomático e formação e acúmulo de H 2 O 2 . Entretanto, tal relação para a cultura sob restrição hídrica ainda não foi estudada. Avaliou-se o movimento estomático e a capacidade antioxidante em mudas de cafeeiro sob diferentes regimes hídricos. Mudas de cafeeiro cv. Catuaí IAC 99, com oito meses de idade, foram submetidas à capacidade de campo, suspensão gradativa e suspensão total da irrigação por um período de 21 dias. Foram realizadas avaliações do potencial hídrico (Ψ w ) foliar na antemanhã e resistência estomática, taxa transpiratória e déficit de pressão de vapor foram avaliados as 10h00 e 17h00. As determinações bioquímicas e enzimáticas foram realizadas em folhas coletadas às 17h00. Todas as avaliações e coletas foram realizadas em intervalos de três dias. Nas plantas em capacidade de campo não houve variação no Ψ w durante o período de avaliação. Para a suspensão gradativa da irrigação, houve queda expressiva a partir dos 12 dias, chegando próximo a -2,5 Mpa, ao final do experimento. Já ...

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Section: Resultsmentioning

confidence: 93%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Stomatal behavior and components of the antioxidative system in coffee plants under water stress

Deuner¹,

Alves²,

Zanandrea

et al. 2011

Sci. agric. (Piracicaba, Braz.)

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“…Of the enzymes involved in the removal of ROS, SOD is usually the first line of defense against oxidant stress (Pompeu et al 2008). The increase in the saline concentration of the irrigation water induced greater SOD activity ( Fig.…”

Section: Resultsmentioning

confidence: 99%

Salt stress: antioxidant activity as a physiological adaptation of onion cultivars

et al. 2013

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The germination and development of several plant species can be limited when those plants are grown in soils with high salinity, which reduces seedling viability and vigor, as well as activating the antioxidant defense system. The aim of this study was to evaluate germination, initial growth and activity of antioxidant enzymes (superoxide dismutase, catalase and ascorbate peroxidase) in seedlings of three onion cultivars (Madrugada, Fepagro 27 and Petroline) exposed to different concentrations of NaCl (0, 40, 80, 120 and 160 mM). Seedlings were evaluated for viability, vigor and antioxidant enzyme activity. The experimental procedures were completely randomized in 3 × 5 factorial design, with each treatment performed in triplicate, at a significance level of 5%. For all cultivars, viability and vigor decreased in parallel with increasing NaCl concentrations, whereas antioxidant enzyme activity increased, and one cultivar (Madrugada) showed less salt tolerance than did the others. We conclude that high NaCl concentrations have a negative effect in the physiological quality of onion seeds, resulting in lower seedling growth rates and increased antioxidant enzyme activity, where Fepagro 27 and Petroline cultivars were more tolerant to salt stress than 'Madrugada' .

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Cadmium‐ and barium‐toxicity effects on growth and antioxidant capacity of soybean (Glycine max L.) plants, grown in two soil types with different physicochemical properties

Melo

Alleoni

Carvalho

et al. 2011

Z. Pflanzenernähr. Bodenk.

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The pollution of agricultural soils by metals is of growing concern worldwide, and is increasingly subject to regulatory limits. However, the effect of metal pollutants on the responses of plants can vary with soil types. In this study, we examined the growth and antioxidant responses of soybean plants exposed to contrasting soils (Oxisol and Entisol), which were artificially contaminated with cadmium (Cd) or barium (Ba). Cadmium reduced plant growth at concentrations higher than 5.2 mg (kg soil)–1, while Ba only affected plant growth at 600 mg kg–1. Such levels are higher than the limits imposed by the Brazilian environmental legislation. Lipid peroxidation was increased only at a Cd concentration of 10.4 mg kg–1 in the Oxisol, after 30 d of exposure. Twelve superoxide dismutase (SOD; EC 1.15.1.1) isoenzymes were evaluated, most of which were classified as Cu/Zn forms. The SOD activity in the leaves of plants grown in the Oxisol decreased over time, whilst remaining high in the Entisol. Catalase (CAT; EC 1.11.1.6) activity in the leaves exhibited little response to Cd or Ba, but increased over time. Glutathione reductase (GR; EC 1.6.4.2) activity was reduced over time when exposed to the higher Cd concentrations, but increased following Ba exposure in the Oxisol. The enzyme‐activity changes were mainly dependent on soil type, time of exposure and, to a lesser extent, the metal concentration of the soil. Soybean plants grown in a sandy soil with a low buffering capacity, such as Entisol, suffer greater oxidative stress than those grown in a clay soil, such as Oxisol.

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Antioxidant isoenzyme responses to nickel-induced stress in tobacco cell suspension culture

Cited by 33 publications

References 19 publications

Stomatal behavior and components of the antioxidative system in coffee plants under water stress

Stomatal behavior and components of the antioxidative system in coffee plants under water stress

Salt stress: antioxidant activity as a physiological adaptation of onion cultivars

Cadmium‐ and barium‐toxicity effects on growth and antioxidant capacity of soybean (Glycine max L.) plants, grown in two soil types with different physicochemical properties

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