Antioxidant Hispidin Derivatives from Medicinal Mushroom Inonotus hispidus

Zan, Li Feng; Qin, Jianchun; Zhang, Ya Mei; Yao, Yan; Bao, Hai Y.; Li, Xiang

doi:10.1248/cpb.59.770

Cited by 57 publications

(43 citation statements)

References 13 publications

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“…High antioxidant activities have also been reported for some mushrooms in Inonotus species such as I. hispidus and I. obliquus [49, 50], but we could not find any published report on the antioxidant capacity of the I. andersonii mushroom. It is worth noting here that the main antioxidative compounds isolated from above-mentioned Inonotus mushrooms are hispidin and hispidin moiety-contained compounds such as inonotusin A and B in I. hispidus [49], inonoblins, and phelligridins in I. obliquus [51]. From these results, we think that I. andersonii may also contain such compounds and this mushroom should be a good candidate for future antioxidant researches.…”

Section: Resultsmentioning

confidence: 60%

“…Until now, such high ORAC values have only been reported for extracts of well-known antioxidant spices like cloves, pimento, and cinnamon [41, 48]. High antioxidant activities have also been reported for some mushrooms in Inonotus species such as I. hispidus and I. obliquus [49, 50], but we could not find any published report on the antioxidant capacity of the I. andersonii mushroom. It is worth noting here that the main antioxidative compounds isolated from above-mentioned Inonotus mushrooms are hispidin and hispidin moiety-contained compounds such as inonotusin A and B in I. hispidus [49], inonoblins, and phelligridins in I. obliquus [51].…”

Section: Resultsmentioning

confidence: 73%

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Wild Mushrooms in Nepal: Some Potential Candidates as Antioxidant and ACE-Inhibition Sources

Bang

Suhara

Doi

et al. 2014

Evidence-Based Complementary and Alternative Medicine

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Twenty-nine mushrooms collected in the mountainous areas of Nepal were analyzed for antioxidant activity by different methods, including Folin-Ciocalteu, ORAC, ABTS, and DPPH assays. Intracellular H2O2-scavenging activity was also performed on HaCaT cells. The results showed that phenolic compounds are the main antioxidant of the mushrooms. Among studied samples, Inonotus andersonii, and Phellinus gilvus exhibited very high antioxidant activity with the phenolic contents up to 310.8 and 258.7 mg GAE/g extracts, respectively. The H2O2-scavenging assay on cells also revealed the potential of these mushrooms in the prevention of oxidative stress. In term of ACE-inhibition, results showed that Phlebia tremellosa would be a novel and promising candidate for antihypertensive studies. This mushroom exhibited even higher in vitro ACE-inhibition activity than Ganoderma lingzhi, with the IC50 values of the two mushrooms being 32 μg/mL and 2 μg/mL, respectively. This is the first time biological activities of mushrooms collected in Nepal were reported. Information from this study should be a valuable reference for future studies on antioxidant and ACE-inhibitory activities of mushrooms.

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Section: Resultsmentioning

confidence: 60%

Section: Resultsmentioning

confidence: 73%

Wild Mushrooms in Nepal: Some Potential Candidates as Antioxidant and ACE-Inhibition Sources

Bang

Suhara

Doi

et al. 2014

Evidence-Based Complementary and Alternative Medicine

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“…Although compound 2 was synthesized previously during synthesis of a biphenyl compound, 15 it was isolated and reported for the first time from a natural source. Compounds 3-7 were identified as 4-(3,4-dihydroxyphenyl)but-3-en-2-one (3), 16 4-hydroxy-3,5-dimethoxybenzoic acid (4), 17 3,4-dihydroxybenzaldehyde (5), 16 4-hydroxybenzene-1,3-dioic acid (6) 18 and 3,4-dihydroxybenzoic acid (7), 19 respectively, based on their physicochemical properties and NMR spectral data, which were in good agreement with those published previously.…”

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confidence: 99%

Phenolic compounds from the fungus Inonotus obliquus and their antioxidant properties

2015

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The medicinal fungus Inonotus obliquus (Pers.: Fr.) Pilat (family Hymenochaetaceae) is widely distributed in Europe, Asia and North America. 1 This mushroom, which lives on tree trunks of Betula (birch) but rarely on Ulmus, Alnus and Fraxinus, has been used as a folk medicine for treating cancer in Russia and western Siberia, and has also been used to prevent and treat heart, liver and stomach diseases and tuberculosis. 2,3 Chemical investigations show that I. obliquus produces a diverse range of secondary metabolites, including phenolic compounds, 4 melanins 5 and lanostane-type triterpenoids. 6 Among these are active components with antioxidant, 4 antitumor 7 and antiviral activities, 8 as well as compounds that improve human immunity against pathogenic microbial infections.Reactive oxygen species are major primary catalysts for initiating oxidation in vivo and in vitro. Reactive oxygen species are associated with many diseases and degenerative processes that occur during aging. 9-11 Accumulating excess reactive oxygen species damages lipids, proteins, carbohydrates and DNA, leading to oxidative stress, loss of cell function and apoptosis or necrosis. 12,13 All of these biochemical processes are common in various diseases, such as cancers, inflammation, atherosclerosis and cardiovascular disease. 14 Therefore, exploring the functions of antioxidants that scavenge reactive oxygen species may be of great value to prevent the onset and propagation of oxidative stress.As part of our search for free radical scavengers from medicinal fungi, two new phenolic antioxidants 1 and 2 were isolated with known compounds 4-(3,4-dihydroxyphenyl)but-3-en-2-one (3), 4-hydroxy-3,5-dimethoxybenzoic acid (4), 3,4-dihydroxybenzaldehyde (5), 4-hydroxybenzene-1,3-dioic acid (6) and 3,4-dihydroxybenzoic acid (7) from a methanol extract of the I. obliquus fruiting body ( Figure 1). In this study, we describe the isolation and structural determination of the phenolic antioxidants from the I. obliquus fruiting body as well as their antioxidant properties.The I. obliquus fruiting bodies were purchased at an herbal drug market in Keumsan, Chungnam Province, Korea. The ground fruiting bodies (5 kg) were extracted twice with 70% aqueous methanol at 60°C for 12 h. After removing the methanol under reduced pressure, the aqueous solution was partitioned between hexane, chloroform, ethyl acetate, and butanol and water, in that order. Compound 1 was purified from the butanol-soluble portion, which was separated by silica gel column chromatography, and eluted with stepwise ethyl acetate: methanol (20:1-1:1, v/v). The phenolic fraction was chromatographed on a Sephadex LH-20 column (Pharmacia, Uppsala, Sweden) eluted with 20% aqueous methanol, followed by preparative HPLC (column: C 18 Cosmosil i.d. 20 × 150 mm, solvent: a gradient of 20-90% aqueous methanol/0.04% trifluoroacetic acid, flow rate: 5 ml per minute) to afford compound 1 (9.1 mg). The ethyl acetate-soluble fraction was subjected to Sephadex LH-20 column chromatography and eluted with 70...

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“…Previous studies of this species have reported the presence of hispolon and hispidin, inonotusin A and B, (E)-4-(3,4-dihydroxyphenyl)but-3-en-2-one, hispidin, 3,4-dihydroxybenzaldehyde, hispindic acids A and B, melanin and polysaccharides with a high content of glucose, mannose, and galactose. [13][14][15][16][17] Extracts from this fungus have exhibited biological activities, including antimicrobial, [18] antiviral, [12] antioxidant, [19] anti-inflammatory, [14] immunomodulatory, [20] antiproliferative, [13] and cytotoxic activities. [21] According to Smolskaite et al, [18] positive results in antimicrobial activity were obtained when I. hispidus was analyzed versus gram-positive bacteria (Bacillus cereus), gramnegative bacteria (Pseudomonas aeruginosa) and fungi (Candida albicans) by means of agar diffusion method.…”

Section: Introductionmentioning

confidence: 99%

A comparative study of the antimicrobial and antioxidant activities of Inonotus hispidus fruit and their mycelia extracts

Angelini

Girometta

Tirillini

et al. 2019

International Journal of Food Properties

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Inonotus hispidus (Bull.) P. Karst. has been used as traditional medicine for the treatment of dyspepsia, cancer, and diabetes. Numerous studies have confirmed the antimicrobial, antiviral, antioxidant, anti-inflammatory, immunomodulatory, antiproliferative and cytotoxic biological activities of extracts from this species. The purpose of this study was a comparative analysis of the antioxidant and the antimicrobial activities of methanol extracts from fruit and liquid-cultured mycelia. Four compounds (N-butylbenzenesulfonamide, lauramidopropyl betaine, 3,5-di-tert-butyl-4-hydroxybenzaldehyde, and uplandicine), determined by hybrid HRMS, were found only in mycelia culture extracts. Free radical scavenging, measured by DPPH assay on methanol extracts, showed an activity of about 17.2% and 22.1% of Trolox in fruiting bodies and mycelia, respectively. The I. hispidus methanol extracts from fruit and mycelia culture were found to have varying degrees of antibacterial and antifungal effects against the pathogenic microorganisms tested (minimum inhibitory concentration from 0.17 to 2.56 μg mL −1 ). ARTICLE HISTORY

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Antioxidant Hispidin Derivatives from Medicinal Mushroom Inonotus hispidus

Cited by 57 publications

References 13 publications

Wild Mushrooms in Nepal: Some Potential Candidates as Antioxidant and ACE-Inhibition Sources

Wild Mushrooms in Nepal: Some Potential Candidates as Antioxidant and ACE-Inhibition Sources

Phenolic compounds from the fungus Inonotus obliquus and their antioxidant properties

A comparative study of the antimicrobial and antioxidant activities of Inonotus hispidus fruit and their mycelia extracts

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