Antioxidant enzyme activities and markers of oxidative stress in the life cycle of earthworm,<i>Eudrilus eugeniae</i>

Kiran, T. Ravi; Aruna, H. K.

doi:10.1080/11250000902932841

Cited by 16 publications

(8 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Studies using earthworms had shown toxic effects of BDE 29 , 30 in soil and thus an increased health risk (WHO 2004) subsequently. 31 The oxidative stress and reduction in thiol components were reported in Eudriluseugeniae 32 stressing the significance of impact of oxidant/antioxidant imbalance. The oxidant burden and the antioxidant depletion observed in the current study were well manifested in the symptoms such as rigidity, coiling, ataxia, constriction in the worms ( Fig.…”

Section: Discussionmentioning

confidence: 99%

Cerebral ganglionic variations and movement behaviors of Lumbricus terrestris on exposure to neurotoxin

Subaraja

Vanisree

2015

ANS

View full text Add to dashboard Cite

BackgroundInvertebrate worms serve as models for understanding the features of neurological functions. Acrylamide (ACR), the well-known neurotoxin, is a water-soluble chemical widely used in various industrial and laboratory processes. ACR is also found in food items which are cooked under high temperature.PurposeThe study attempts to assess the neuropathological changes in cerebral ganglions along with the locomotion and neuronal behavior of Lumbricus terrestris on ACR intoxication.MethodsThe dosage of acrylamide induced neurotoxicity ranged from 0–17.5 mg/kg body weight for 7 days. The time/dose dependent changes in the oxidant and antioxidant status, activities of Na+/K+ATPase, Ca2+/Mg2+ ATPase and 5’ Nucleotidase were assessed along with the locomotor behavioral analysis.ResultThe activities of super oxidase dismutase and catalases were not altered appreciably. However, the glutathione family, lipid peroxide, protein carbonyl content and vitamin C did show significant variations (p<0.001) in a dose-dependent manner, depicting more of oxidative stress, when compared to control worms. The activities of Na+/K+ ATPase was significantly affected (p<0.001) at 3.5 mg/kg bw itself while those of both Ca2+ and 5’ Nucleotidase were found to be affected at 7.0 mg/kg bw of ACR. Mg2+ ATPase showed significant reduction (p<0.001) in its activity only at 10.5 mg/kg bw of ACR. These dose dependent biochemical variations observed were found to be linked with the behavior of the worms as evident from the latency of movement in a dose-dependent manner which is less pronounced at 7.0 mg and more pronounced at 17.5 mg/kg bw of ACR.ConclusionThe study suggests that ACR disrupts GSSS/GSH balance and perturbs ionic homeostasis in worms and thus affect the motor function highlighting their (GSH-ions) interrelationship in influencing neuromuscular activity. These simple analyses implicate that the cerebral ganglionic variations in the worms may be useful to appreciate the pathology of the neurological diseases (provided sophisticated analyses are employed) especially which involve movement dysfunction, where the brain tissue samples from the affected human patients are scarce.

show abstract

Section: Discussionmentioning

confidence: 99%

Cerebral ganglionic variations and movement behaviors of Lumbricus terrestris on exposure to neurotoxin

Subaraja

Vanisree

2015

ANS

View full text Add to dashboard Cite

show abstract

“…Animals were sacri ced immediately after exposure for 14 d to the four doses of the pesticides. Tissues were processed for biochemical study following the methods of Avi Kiran and Aruna [20] Enzymes assays were determined following the methods below…”

Section: Sample Collection and Processingmentioning

confidence: 99%

Responses of Earthworms to Acetamiprid

Erhunmwunse,

Tongo,

Endurance

et al. 2023

Preprint

View full text Add to dashboard Cite

To increase agricultural output, millions of tonnes of neonicotinoids are reportedly used worldwide, but these agrochemicals have negative impacts on soil quality and biodiversity. 98% of pesticides applied reach non-target organisms in the water and soil including the earthworms. Earthworm provides significant contributions to soil fertility and serves as a bioindicator for pesticide-induced soil pollution in an ecotoxicological investigation. In this study, earthworms (Aporrectodea longa) were subjected to commercial-grade Acetaminprid at various doses for 14 days and the LC50 was found to be 2.52 mg/kg. Morphological alterations and behavioral changes such as weight loss, avoidance, and growth rate were observed to be concentration-dependent. Catalase (CAT), glutathione-S-transferase (GST), glutathione peroxidase (GPx), C-reactive protein (CRP), and Total Oxidative Capacity (TAC) activities were also measured. Significant alterations in these stress markers were detected in pesticide-exposed earthworms at increasing pesticide concentrations. Our findings showed that the exposed earthworm's growth rate decreased, its digging activity slowed down, it died, and it lost a significant amount of weight. These findings varied significantly depending on the pesticide concentration. This study indicates that Earthworms are particularly susceptible to acetamiprid, which cause immobility and rigidity, as well as a reduction of biomass and growth by disturbing numerous physiological activities, resulting in a loss of the earthworm population.

show abstract

“…To initiate the reaction, H 2 O 2 was added to the mixture. The decrease in absorbance was taken at 470 nm (Ravi Kiran & Aruna, 2010).…”

Section: Analysis Of Antioxidant Enzymesmentioning

confidence: 99%

The potential of Bacillus subtilis and phosphorus in improving the growth of wheat under chromium stress

Ilyas

Akhtar

Naseem

et al. 2022

Journal of Applied Microbiology

View full text Add to dashboard Cite

The high magnitude of heavy metals contamination in daily food attracts the attention of researchers for nding e cient and eco-friendly approaches that can help resolve this problem. Hexavalent Chromium (Cr +6 ) is one of the most toxic heavy metals that have deteriorating effects on the growth and quality of the end product of wheat. In this research, the role of Bacillus subtilis and phosphorus fertilizer on wheat development in Cr +6 stress was evaluated. The statistical analysis of the data showed that the coapplication of Bacillus subtilis and phosphorus gave considerably signi cant (p<0.05) results compared to their separate application. The increase in shoot length, root length, shoot fresh weight, and root fresh weight was 38.17%, 29.31%, 47.89%, and 45.85%, respectively, compared to untreated stress-facing plants. The use of Bacillus subtilis and phosphorus improved the process of osmolytes (proline 39.98%, and sugar 41.30%) production in plants which results in high water uptake and structural stability of proteins (86.65%) and improved cell membranes (66.66%) as well. They also augmented the production of antioxidants by 67.71% (SOD), 95.39% (APX), and 60.88% (CAT), respectively. The Cr +6 accumulations were reduced by 54.24%, 59.19%, and 90.26% in the shoot, root, and wheat grains, respectively. The combined application of Bacillus subtilis and phosphorus can reduce the heavy metals toxicity in crops.

show abstract

Antioxidant enzyme activities and markers of oxidative stress in the life cycle of earthworm,Eudrilus eugeniae

Cited by 16 publications

References 32 publications

Cerebral ganglionic variations and movement behaviors of Lumbricus terrestris on exposure to neurotoxin

Cerebral ganglionic variations and movement behaviors of Lumbricus terrestris on exposure to neurotoxin

Responses of Earthworms to Acetamiprid

The potential of Bacillus subtilis and phosphorus in improving the growth of wheat under chromium stress

Contact Info

Product

Resources

About