Antioxidant effects of the essential oil ofSyzygium aromaticumon bovine epididymal spermatozoa

Abstract

Focusing on its application in reproductive biotechnology, we evaluated the effects of the essential oil of Syzygium aromaticum (EOSA) on bovine epididymal sperm quality variables, including morphology, membrane functional integrity, membrane structural integrity, mitochondrial activity, metabolic activity, motility and oxidative stress by reactive oxygen species (ROS) levels. Bovine spermatozoa from eight males were incubated into the following groups: EOSA0 (without EOSA), EOSA10 (10 μg/ml of EOSA), EOSA15 (15 μg/ml of EOSA) and EOSA20 (20 μg/ml of EOSA); the incubation time with and without the EOSA was 1 or 6 hr. None of the sperm quality variables presented difference among the EOSA concentrations. However, the incubation time had a significant effect on the membrane functional integrity, membrane structural integrity, mitochondrial activity, progressive motility and some kinetic parameters. The effect of interaction among EOSA and incubation time was significant only on ROS levels. Spermatozoa incubated in the presence of 15 μg/ml of the EOSA for 1 hr had significantly reduced ROS levels compared with all other groups in the same time. In conclusion, the EOSA at a concentration of 15 µg/ml has antioxidant effects and protects bovine epididymal spermatozoa; hence, the EOSA may potentially be used in the field of reproductive biotechnology.

Preserving effect of Loboob (a traditional multi-herbal formulation) on sperm parameters of male rats with busulfan-induced subfertility

“…(almond) enhances sperm motility via its unsaturated fatty acid component (Hussein & Raheem, 2015). Protecting structural and functional membrane integrity as well as mitochondrial effects are the mechanisms described for Syzygium aromaticum (cloves) as a dose-dependent component (Mishra & Singh, 2016;Santos et al, 2019). Protecting structural and functional membrane integrity as well as mitochondrial effects are the mechanisms described for Syzygium aromaticum (cloves) as a dose- dependent component (36, 37).…”

“…(almond) enhances sperm motility via its unsaturated fatty acid component ( Hussein & Raheem, 2015 ). Protecting structural and functional membrane integrity as well as mitochondrial effects are the mechanisms described for Syzygium aromaticum (cloves) as a dose-dependent component ( Mishra & Singh, 2016 ; Santos et al. , 2019 ).…”

“…(35). Besides, Syzygium aromaticum positively affects sperm viability by protecting structural and functional membrane integrity ( Santos et al ., 2019 ). It should be considered that Piper.…”

Implications of cryopreservation on structural and functional attributes of bovine spermatozoa: An overview

“…The clove oil has sperm protective activity and has shown good ROS‐sequestering activity in both biochemical and cellular assays (Baghshahi et al., 2014). The essential oils such as olive and clove oil may therefore be an agent of interest for the maintenance of sperm quality during in vitro reproductive biotechnology (Santos et al., 2019).…”

Preliminary Assessment of the Mucosal Toxicity of Tea Tree (Melaleuca alternifolia) and Rosemary (Rosmarinus officinalis) Essential Oils on Novel Porcine Uterus Models

“…During the last decades, physical approaches were developed such as bacteria removal by colloid centrifugation [10] and microfiltration of seminal plasma [11], or the use of antimicrobial peptides [12,13]. At the same time, natural compounds, and in particular essential oils (EOs) were evaluated on semen of different species for their antioxidant and antimicrobial proprieties [14][15][16][17].…”