Antioxidant Effect of Melatonin in Human Retinal Neuron Cultures

Lee, Min-Cheol; Chung, Young-Taek; Lee, Jae-Hyuk; Jung, Jong-Jae; Kim, Hyung-Seok; Kim, Seung Up

doi:10.1006/exnr.2001.7793

Cited by 12 publications

(9 citation statements)

References 34 publications

(31 reference statements)

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“…Melatonin has been shown to be neuroprotective in different experimental models of acute (Duan et al, 2006) and degenerative (Sharma et al, 2006;Rochitta et al, 2006) brain disorder, as well as to have antioxidant properties protecting against cellular injury induced by reactive oxygen species in astrocytes and retinal neuron cultures (Lee et al, 2001). Melatonin has excellent pharmacological properties after oral or parenteral administration, and it easily crosses the blood-brain barrier.…”

Section: Discussionmentioning

confidence: 99%

“…Melatonin also reduced acute (Ohta et al, 1999) and chronic (Cruz et al, 2005) toxic liver injury, as well as oxidative stress-related apoptosis, in experimental cholestasis (Padillo et al, 2004) and cirrhosis (Cruz et al, 2005). Melatonin exerts antioxidant properties against cellular injury induced by reactive oxygen species in retinal neuron cultures (Lee et al, 2001). Nevertheless, the role of melatonin in the prevention of neurological damage by oxidative stress in experimental OJ has not been tested.…”

mentioning

confidence: 97%

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Melatonin prevents brain oxidative stress induced by obstructive jaundice in rats

Cruz

Túnez

Martínez

et al. 2007

J of Neuroscience Research

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The aim of the study was to analyze the impact of melatonin on brain oxidative stress in experimental biliary obstruction. Cholestasis was done by a double ligature and section of the extrahepatic biliary duct. Melatonin was injected intraperitoneally (500 microg/kg/day). Malondialdehyde (MDA), reduced glutathione (GSH), catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GPx) contents were determined in the brain tissue. Biliary obstruction raised MDA and reduced GSH contents in the cortex, cerebellum, and hypothalamus areas. Moreover, the scavenger enzyme activity significantly dropped in all areas of the brain. Melatonin drastically reduced MDA concentration and enhanced GSH concentration, as well as all antioxidant enzyme activity in all brain areas obtained from the bile duct-ligated animals. In conclusion, the treatment with melatonin decreased lipid peroxidation and recovered the antioxidant status in the brain from cholestatic animals.

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 97%

Melatonin prevents brain oxidative stress induced by obstructive jaundice in rats

Cruz

Túnez

Martínez

et al. 2007

J of Neuroscience Research

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“…In order to measure viability of the B2A1 cells, the 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) colorimetric assay was performed (16). This method is based on the cellular reduction of tetrazolium salt MTT (Sigma) by the mitochondrial dehydrogenase of viable cells, which results in formation of a blue formazan product which can then be measured with a spectrophotometer.…”

Section: Methodsmentioning

confidence: 99%

Neurotoxicity Screening in a Multipotent Neural Stem Cell Line Established from the Mouse Brain

et al. 2010

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Neural stem cells (NSCs) have mainly been applied to neurodegeneration in some medically intractable neurologic diseases. In this study, we established a novel NSC line and investigated the cytotoxic responses of NSCs to exogenous neurotoxicants, glutamates and reactive oxygen species (ROS). A multipotent NSC line, B2A1 cells, was established from long-term primary cultures of oligodendrocyte-enriched cells from an adult BALB/c mouse brain. B2A1 cells could be differentiated into neuronal, astrocytic and oligodendroglial lineages. The cells also expressed genotypic mRNA messages for both neural progenitor cells and differentiated neuronoglial cells. B2A1 cells treated with hydrogen peroxide and L-buthionine-(S,R)-sulfoximine underwent 30-40% cell death, while B2A1 cells treated with glutamate and kainate showed 25-35% cell death. Cytopathologic changes consisting of swollen cell bodies, loss of cytoplasmic processes, and nuclear chromatin disintegration, developed after exposure to both ROS and excitotoxic chemicals. These results suggest that B2A1 cells may be useful in the study of NSC biology and may constitute an effective neurotoxicity screening system for ROS and excitotoxic chemicals.

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“…12,13 In vitro, melatonin is generally regarded as a cytoprotectant and appears to shield the retina from oxidative damage. [14][15][16][17][18] Importantly, if neuroleptics mediate retinopathy through dopamine-receptor blockade, then many other neuroleptic agents should also produce retinopathies-they do not. Risperidone, ritanserin, CPZ, TRZ, and haloperidol have similar binding affinity ratios for D2 and D4 receptors, yet only CPZ and TRZ produce retinopathies.…”

Section: Potential Mechanisms For Drug-induced Retinopathymentioning

confidence: 98%

Invited Review: Fluphenazine Augments Retinal Oxidative Stress

Toler

2005

Journal of Ocular Pharmacology and Therapeutics

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Recently, fluphenazine, a phenothiazine neuroleptic, has been associated with idiosyncratic retinopathy. Neuroleptic-induced retinopathy appears to be isolated to only a few structurally related phenothiazines, suggesting that the causality is not the result of dopamine antagonism. The chemical structure of fluphenazine is very similar to that of chlorpromazine and thioridazine, agents known to produce retinopathy. Like chlorpromazine and thioridazine, fluphenazine may be oxidized by retinal cytochrome P450 and/or myeloperoxidase to an electrophile, producing injury in susceptible patients.

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Antioxidant Effect of Melatonin in Human Retinal Neuron Cultures

Cited by 12 publications

References 34 publications

Melatonin prevents brain oxidative stress induced by obstructive jaundice in rats

Melatonin prevents brain oxidative stress induced by obstructive jaundice in rats

Neurotoxicity Screening in a Multipotent Neural Stem Cell Line Established from the Mouse Brain

Invited Review: Fluphenazine Augments Retinal Oxidative Stress

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