Antioxidant, Antihypertensive and Antimicrobial Properties of Phenolic Compounds Obtained from Native Plants by Different Extraction Methods

Boy, Francisco Ramiro; Casquete, Rocío; Martínez, Ana Torrecillas; Córdoba, Maria de Guı́a; Ruíz-Moyano, Santiago; Benito, María José

doi:10.3390/ijerph18052475

Cited by 15 publications

(5 citation statements)

References 43 publications

(45 reference statements)

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“…The celery extract obtained from different extraction techniques and solvents had ACE inhibition activity with no significant difference. These results were different from previous studies that reported that several extracts of native plants from the Extremadura region (Spain) produced from the UAE had higher ACE inhibitory activity than those extracted using agitated maceration [54]. Several traditional medicine industries for standardized herbal medicine and phytopharmaca in Indonesia also use celery as a single ingredient or a mixture with other medicinal plants as an antihypertensive product.…”

Section: Discussioncontrasting

confidence: 99%

Biological Activity of Celery Extract Using Different Extraction Methods

Septiana¹,

Rahmawati²,

Izzati³

et al. 2023

Proceedings of the 1st International Conference for Health Research – BRIN (ICHR 2022)

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Celery is a medicinal plant that is widely used as an ingredient in traditional medicine in Indonesia. Scientifically, celery extract has the ability as an antioxidant, antibacterial, anti-breast cancer, and inhibiting ACE enzyme activity as antihypertensive. The use of maceration and ultrasound-assisted extraction (UAE) methods will give different results on the amount and biological activity of the extract. This study aims to determine the biological activity of celery extract using maceration and ultrasound-assisted extraction method. TEAC, FRAP, NO, and DPPH scavenging assays were used to determine in vitro antioxidant activities. Folin-Ciocalteu reagent and aluminium chloride colorimetric assay were used to determine total phenolic and flavonoid contents, respectively. The sulforhodamine B and angiotensin-converting enzyme (ACE) inhibition assays were used to determine anti-breast cancer and antihypertensive activities, respectively. The celery extract from both extraction methods had antioxidants activity, inhibited the growth of MDA-MB-231 breast cancer cell line, and showed as antihypertensive by ACE inhibition. The maceration gave better activities than UAE with significant results in antioxidants, especially in a water solvent. The use of solvents had more effect on antioxidant activity, especially the FRAP and DPPH free radical scavenging method, as well as the TPC and TFC values of the extract compared to the extraction technique. It was found that ethanol solvent was better than water. The maceration provided better activities than ultrasound-assisted extraction in antibreast cancer. However, there was no significant difference in antihypertensives. The UAE extraction method produced higher yields but had lower antioxidant and anticancer activity than a maceration.

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Section: Discussioncontrasting

confidence: 99%

Biological Activity of Celery Extract Using Different Extraction Methods

Septiana¹,

Rahmawati²,

Izzati³

et al. 2023

Proceedings of the 1st International Conference for Health Research – BRIN (ICHR 2022)

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“…Regarding the antimicrobial activity of Q. ilex extracts, few studies are available in the literature ( Table S1 ). As for leaf extracts, Boy et al [ 23 ] evaluated the efficacy of Q. ilex leaf extracts obtained by ultrasonication in 90% ( v / v ) ethanol-water against yeasts responsible for food spoilage, finding a high inhibition capacity against Candida boidinii C. ramirez , Priceomyces carsonii (Phaff et Knapp) Suzuki et Kurtzman , Kregervanrija fluxuum , (Phaff and E.P. Knapp) Kurtzman and Zygosacharomyces bailii (Lindner) Guillierm at a concentration of 2000 µg·mL −1 , attributed to the high content of phenolic compounds; Sánchez-Gutiérrez et al [ 24 ] demonstrated the antibacterial activity of an aqueous extract of Q. ilex leaves obtained by a microwave-assisted extraction procedure against foodborne Gram- and Gram+ bacteria with inhibition values in the 1000 to 5000 µg·mL −1 range; and Güllüce et al [ 25 ] tested in vitro a methanol extract of Q. ilex leaves against a wide range of human and plant-associated microorganisms, including Pseudomonas syringae Van Hall 1904, with a MIC of 250 µg·mL −1 .…”

Section: Discussionmentioning

confidence: 99%

Holm Oak (Quercus ilex subsp. ballota (Desf.) Samp.) Bark Aqueous Ammonia Extract for the Control of Invasive Forest Pathogens

Sánchez-Hernández

Balduque-Gil

Barriuso-Vargas

et al. 2022

IJMS

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Holm oak (Quercus ilex subsp. ballota (Desf.) Samp.) bark is a commonly used remedy to treat gastrointestinal disorders, throat and skin infections, hemorrhages, and dysentery. It has also been previously reported that its methanol extracts possess antibacterial activity, which can be related to the richness of Quercus spp. extracts in phenolic compounds, such as flavonoids and tannins. However, there is no information on the antifungal (including oomycete) properties of the bark from Q. ilex or its subspecies (ilex and ballota). In this work, we report the characterization of the aqueous ammonia extract of its bark by FTIR and GC-MS and the results of in vitro and ex situ inhibition tests against three phytopathogens. The main phytochemical components identified were inositols (19.5%), trans-squalene (13%), 4-butoxy-1-butanol (11.4%), gulopyranose (9.6%), lyxose (6.5%), 2,4-dimethyl-benzo[H]quinoline (5.1%), catechol (4.5%), and methoxyphenols (4.2%). The efficacy of the extract in controlling forest phytopathogens was tested in vitro against Fusarium circinatum (responsible for pitch canker of Pinus spp.), Cryphonectria parasitica (which causes chestnut blight), and Phytophthora cinnamomi (which causes ‘root and crown rot’ in a variety of hosts, including Castanea, conifers, Eucalyptus, Fagus, Juglans, Quercus, etc.), obtaining EC90 values of 322, 295, and 75 μg·mL−1, respectively, much lower than those attained for a commercial strobilurin fungicide (azoxystrobin). The extract was further tested ex situ against P. cinnamomi on artificially inoculated, excised stems of ‘Garnem’ almond rootstock, attaining complete protection at a dose of 782 μg·mL−1. The results suggest that holm oak bark extract may be a promising source of bioactive compounds against invasive forest pathogens, including the oomycete that is causing its decline, the so-called ‘seca’ in Spain.

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“…Polyphenols are a category of secondary plant metabolites that incorporates a large number and variety of compounds ( Table 1 ) [ 6 , 7 , 8 ]. Such a variety of compounds incorporates a great variety of possible benefits, being able to find several beneficial effects for the same subclass and common effects in different classes and subclasses [ 9 , 10 , 11 ]. Of all the polyphenols studied as nutraceuticals or functional ingredients, we can highlight those that are better known as curcuminoids, betalains, and stilbenes, such as resveratrol, quinones as ubiquinol, and various flavonoids very studied, such as luteolin, genistein, hesperetin, quercetin, catechinsm and anthocyanidins ( Table 1 ) [ 12 , 13 ].…”

Section: Introductionmentioning

confidence: 99%

Essential Oils and Melatonin as Functional Ingredients in Dogs

Ruiz-Cano¹,

Sánchez-Carrasco²,

Mihyaoui

et al. 2022

Animals

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The use of nutraceuticals or functional ingredients is increasingly widespread in human food; their use is also widespread in animal feed. These natural compounds generally come from plant materials and comprise a wide range of substances of a very diverse chemical nature. In animals, these compounds, so-called phytogenics, are used to obtain improvements in feed production/stability and also as functional components with repercussions on animal health. Along with polyphenols, isoprenoid compounds represent a family of substances with wide applications in therapy and pet nutrition. Essential oils (EOs) are a group of complex substances with fat-soluble nature that are widely used. Melatonin is an indolic amine present in all living with amphiphilic nature. In this work, we present a review of the most relevant phytogenics (polyphenol, isoprenoid, and alkaloid compounds), their characteristics, and possible uses as nutraceuticals in dogs, with special emphasis on EOs and their regulatory aspects, applied in foods and topically. Additionally, a presentation of the importance of the use of melatonin in dogs is developed, giving physiological and practical aspects about its use in dog feeding and also in topical application, with examples and future projections. This review points to the combination of EOs and melatonin in food supplements and in the topical application as an innovative product and shows excellent perspectives aimed at addressing dysfunctions in pets, such as the treatment of stress and anxiety, sleep disorders, alopecia, and hair growth problems, among others.

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Antioxidant, Antihypertensive and Antimicrobial Properties of Phenolic Compounds Obtained from Native Plants by Different Extraction Methods

Cited by 15 publications

References 43 publications

Biological Activity of Celery Extract Using Different Extraction Methods

Biological Activity of Celery Extract Using Different Extraction Methods

Holm Oak (Quercus ilex subsp. ballota (Desf.) Samp.) Bark Aqueous Ammonia Extract for the Control of Invasive Forest Pathogens

Essential Oils and Melatonin as Functional Ingredients in Dogs

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