Antioxidant, antibacterial and in vivo wound healing properties of laminaran purified from Cystoseira barbata seaweed

Sellimi, Sabrine; Maâlej, Hana; Rekik, Dorsaf Moalla; Benslima, Abdelkarim; Ksouda, Ghada; Hamdi, Marwa; Sahnoun, Zouheir; Li, Suming; Nasri, Monçef; Hajji, Mohamed

doi:10.1016/j.ijbiomac.2018.07.171

Cited by 90 publications

(52 citation statements)

References 54 publications

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“…Lipids are traditionally removed by lower polarity solvents, such as chloroform, petroleum ether or dichloromethane; pigments with semipolar solvents, such as acetone, methanol or ethanol; while other high polarity molecules, such as monosaccharides, proteins and minerals are extracted in water [20]. Prior to laminarin extraction from Cystoseira barbata, Sellimi et al [42] seaweed powder was sequentially treated twice with acetone-methanol (7:3) and twice with chloroform for 24 h at 30 • C with constant stirring (250 rpm). Similarly, January et al [43] used the mixture of methanol-chloroform-water (4:2:1) as a pre-treatment prior to fucoidan extraction.…”

Section: Pre-treatment Of Marine Brown Algaementioning

confidence: 99%

“…Furthermore, for the improvement of the extraction yield, the use of 0.1 M HCl solution has been shown to be effective since it enables cell walls hydrolysis and facilitates fucoidan and laminarin extraction from the matrix [42,51]. In addition, the acid converts alginate into water-insoluble alginic acid, which is removed, together with solid seaweeds residues, resulting in relatively pure fucoidan fraction [20].…”

Section: Conventional Extraction Technique (Ce)mentioning

confidence: 99%

“…Ethanol precipitation is often used as the first step in APS purification, which removes low molecular weight impurities from the polysaccharides. Numerous researchers conducted purification step by adding two or three volumes of ethanol to the extract and allowing the mixture to precipitate overnight at 4 • C [16,42,46,52,54]. Due to shielded oppositely charged groups APS are soluble in solvents with higher dielectric constants such as water.…”

Section: Purification Proceduresmentioning

confidence: 99%

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Advanced Technologies for the Extraction of Marine Brown Algal Polysaccharides

et al. 2020

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Over the years, brown algae bioactive polysaccharides laminarin, alginate and fucoidan have been isolated and used in functional foods, cosmeceutical and pharmaceutical industries. The extraction process of these polysaccharides includes several complex and time-consuming steps and the correct adjustment of extraction parameters (e.g., time, temperature, power, pressure, solvent and sample to solvent ratio) greatly influences the yield, physical, chemical and biochemical properties as well as their biological activities. This review includes the most recent conventional procedures for brown algae polysaccharides extraction along with advanced extraction techniques (microwave-assisted extraction, ultrasound assisted extraction, pressurized liquid extraction and enzymes assisted extraction) which can effectively improve extraction process. The influence of these extraction techniques and their individual parameters on yield, chemical structure and biological activities from the most current literature is discussed, along with their potential for commercial applications as bioactive compounds and drug delivery systems.(APS) is determined by algae species however it is also influenced by other factors causing inter-species variation, e.g., growth location and harvesting season [8]. Vast structural variation between the APS therefore presents a challenge in terms of pre-treatments application, extraction techniques and optimization, characterization of isolated fractions and determination of their biological properties.Chemical structure and yield of APS isolated from marine macroalgae by conventional extraction (CE) techniques can be affected by various experimental conditions (pH, time, temperature, pressure, particle size, solvent, sample to solvent ratio, agitation speed etc.). In addition, different advanced techniques such as microwave assisted extraction (MAE), ultrasound assisted extraction (UAE), pressurized liquid extraction (PLE), enzyme-assisted extractions (EAE) are assessed and applied for APS extraction [9][10][11].In general, the chemical structure of polysaccharides determines its physical, chemical and biochemical properties as well as its biological activities [12]. Several studies have reported that their biological activity is strongly associated with their chemical structure [9]. Due to very complex mechanisms that are affected by many factors, the correlation between polysaccharide structure and biological activity is still not sufficiently clarified.In order to improve isolation of APS, pre-treatments are usually applied to the algal biomass prior to the extraction process with the two aims: (i) to prevent co-extraction of interfering bioactive compounds with similar solubility; and (ii) to disrupt cell walls and improve mass transfer of APS into extraction solvent. The first type of pre-treatments is therefore used to remove compounds which are highly bound to the APS such as proteins, phenols and lipids, as well as mannitol and chlorophyll [13]. For that purpose, the application of various pr...

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Section: Pre-treatment Of Marine Brown Algaementioning

confidence: 99%

Section: Conventional Extraction Technique (Ce)mentioning

confidence: 99%

Section: Purification Proceduresmentioning

confidence: 99%

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Advanced Technologies for the Extraction of Marine Brown Algal Polysaccharides

et al. 2020

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“…It was observed that the formulated cream comprising laminaran as a bioactive constituent exerted significant wound-improving effects, and after 13 days, the wound reduction reached 98.57%. As compared with the untreated groups, the derma in laminaran-treated group was correctly organized revealing increased fibroblast densities as well as enhanced deposition of collagen [44]. Very recently, fucoidan, from brown algae, has been used for transdermal formulations targeting inflammatory skin conditions or as an anticoagulant after topical application, which is a very important aspect of wound healing [39,45].…”

Section: Wound-curative Potentialmentioning

confidence: 99%

Marine Seaweed Polysaccharides-Based Engineered Cues for the Modern Biomedical Sector

Bilal

Iqbal

2019

Marine Drugs

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Seaweed-derived polysaccharides with unique structural and functional entities have gained special research attention in the current medical sector. Seaweed polysaccharides have been or being used to engineer novel cues with biomedical values to tackle in practice the limitations of counterparts which have become ineffective for 21st-century settings. The inherited features of seaweed polysaccharides, such as those of a biologically tunable, biocompatible, biodegradable, renewable, and non-toxic nature, urge researchers to use them to design therapeutically effective, efficient, controlled delivery, patient-compliant, and age-compliant drug delivery platforms. Based on their significant retention capabilities, tunable active units, swelling, and colloidal features, seaweed polysaccharides have appeared as highly useful materials for modulating drug-delivery and tissue-engineering systems. This paper presents a standard methodological approach to review the literature using inclusion-exclusion criteria, which is mostly ignored in the reported literature. Following that, numerous marine-based seaweed polysaccharides are discussed with suitable examples. For the applied perspectives, part of the review is focused on the biomedical values, i.e., targeted drug delivery, wound-curative potential, anticancer potentialities, tissue-engineering aspects, and ultraviolet (UV) protectant potential of seaweed polysaccharides based engineered cues. Finally, current challenges, gaps, and future perspectives have been included in this review.

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“…Thus, the use of antioxidant substances minimizes the generated damage and guarantees a conducive environment to healing. [46][47][48][49] It is reported in the literature 50 that polysaccharides isolated from Agaricus brasiliensis mushrooms were able to reduce tissue expression of mRNA (messenger ribonucleic acid) of the proinflammatory cytokine IL-1β in burns, favoring the repair process of injured tissue.…”

Section: Ferrous Ion Chelating Assaymentioning

confidence: 99%

Chitosan and Agaricus brasiliensis Polysaccharides Films: A Preliminary Study

Gonzaga

Campelo

Saraiva

et al. 2020

J. Braz. Chem. Soc.

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The known biotechnological properties of chitosan and the recent biological activities attributed to polysaccharides from Agaricus brasiliensis have been of interest to obtain films constituted by these two polymers. The glucans obtained from the mushroom inhibited about 96.5% of the ferrous ion, besides not promoting a significant increase of lactate dehydrogenase enzyme (LDH), which indicates that the polysaccharide is able to inhibit the production of radical species and also presents low cytotoxicity to the biological systems. The results of spectroscopy analyses in the infrared region (FTIR) and X-ray diffraction suggest an existing electrostatic interaction between the substances. A reduction in the films' swelling capacity was observed with an increase in the polysaccharide content in the composition. In addition, scanning electron microscopy (SEM) revealed greater surface density of the films. In convening the biological properties of the substances, it is expected that this study raises interest in evaluating the films and their capacity for healing wounds and burns.

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Antioxidant, antibacterial and in vivo wound healing properties of laminaran purified from Cystoseira barbata seaweed

Cited by 90 publications

References 54 publications

Advanced Technologies for the Extraction of Marine Brown Algal Polysaccharides

Advanced Technologies for the Extraction of Marine Brown Algal Polysaccharides

Marine Seaweed Polysaccharides-Based Engineered Cues for the Modern Biomedical Sector

Chitosan and Agaricus brasiliensis Polysaccharides Films: A Preliminary Study

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