Antioxidant, anti-inflammatory and anticoagulant activities of three Thymus species grown in southeastern Morocco

Abstract

Background: Thyme has been used for centuries in southeastern Morocco to treat a wide range of diseases such as inflammation disorders. The aim of the current study is to examine and to compare in vitro the anti-inflammatory, antioxidant, and anticoagulant activities of three thyme species grown in southeastern Morocco. Results: Data showed that all studied species possess an important antioxidant activity: Thymus atlanticus (IC 50 = 16.59 μg/mL), Thymus zygis (IC 50 = 15.43 μg/mL), and Thymus satureioides (IC 50 = 14.65 μg/mL). Concerning the antiinflammatory activity, the highest effect was depicted in Thymus atlanticus followed by Thymus zygis and Thymus satureioides. With regard to the anticoagulant activity, the aqueous extract of these species prolongs activated partial thromboplastin time, prothrombin time, and thrombin time significantly (p < 0.05) in a dose-dependent manner. Conclusion: Our results provide evidence that thymus extract exhibits marked antioxidant, anticoagulant, and antiinflammatory effects, thus justifying the popular uses of these plants to treat some inflammatory and cardiovascular illnesses.

In-vitro antioxidant, anti-cancer, and anti-inflammatory activities of selected medicinal plants from western Nepal

“…Free radical scavenging was determined by 1,1-diphenyl 1-2-picryl-hydrazyl (DPPH) assay [43]. For the measurement of antioxidant activity, 1 mL of plant extracts (10,20,40,60,80, 100 μg/mL) was mixed with 3 mL of the DPPH solution of 100 mM concentration. The reaction mixtures were incubated for 30 min in dark and absorbance was recorded at 517 nm using a UV-visible spectrophotometer.…”

“…In addition, the 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) cation decolorization assay was also performed for the evaluation of antioxidant potential of the plant extracts by using standard procedures followed by the previous investigators slight modifications [44]. An aliquot of 1 mL of plant extract (10,20,40,60,80, 100 μg/mL) was mixed with 3 mL of ABTS working solution and incubated for 10 min in dark. The absorbance was taken at 734 nm.…”

“…Different epidemiological and pharmacological studies suggested that the daily intake of certain phytochemicals can reduce the incidence of several types of cancer [18,19]. Furthermore, inflammation and oxidative stress have been reported to play an important role in the development of several diseases [20]. Although, anti-inflammatory agents such as nonsteroidal anti-inflammatory drugs (NSAID) are used for the treatment of inflammatory diseases, suffer from the side effect such as gastro-toxicity, hence it is an utmost need of development of safer anti-inflammatory agents [21].…”

Effect of Aqueous Extract and Polyphenol Fraction Derived from Thymus atlanticus Leaves on Acute Hyperlipidemia in the Syrian Golden Hamsters

“…Moreover, it was reported that the aqueous extract of T. atlanticus possesses high total phenolic content and exhibited a potent anti-inflammatory effect in animal models of inflammation and inhibits blood coagulation ex vivo and in vivo [12,13]. In addition, the aqueous extract of this plant showed potent in vitro antioxidant activity, but, to our knowledge, its antioxidant activity in vivo has not yet been studied [5,13,14]. A preliminary screening of some ymus species from Morocco for hypolipidemic activity indicated that T. atlanticus showed possible hypolipidemic effects in a rat model [5].…”

Health-Promoting Effects of Thymus Phenolic-Rich Extracts: Antioxidant, Anti-inflammatory and Antitumoral Properties

“…Interestingly, the authors disclosed a greater antioxidant ability of these polar solvent extracts in comparison to non-polar ones [56]. Different results were described for aqueous extracts of Thymus atlanticus, T. zygis and Thymus satureioides based on ABTS •+ scavenging assay, which corresponded to about 7.5-8.5 times those of the standard ascorbic acid [62].…”