Antioxidant and enzymatic responses to oxidative stress induced by pre-harvest water supply reduction and ripening on mango (Mangifera indica L. cv. ‘Cogshall’) in relation to carotenoid content

Abstract

The effects of a reduction in water supply during fruit development and postharvest fruit ripening on the oxidative status and the antioxidant defense system were studied in the mango fruit (Mangifera indica L.) cv. Cogshall. Changes in non-enzymatic (ascorbate) and enzymatic (SOD, CAT, APX, MDHAR, DHAR and GR) antioxidants, as well as oxidative parameters (H2O2 and MDA) and major carotenoids, were measured in unripe and ripe fruits from well-irrigated and non-irrigated trees. Under non-limiting water supply conditions, ripening induced oxidation as a result of the production of ROS and decreased ascorbate content. Antioxidant enzymatic systems were activated to protect fruit tissues and to regenerate the ascorbate pool. The carotenoid pool, mainly represented by β-carotene and esterified violaxanthine isomers, accumulated naturally during mango ripening. The suppression of irrigation decreased fruit size and induced accumulation of ABA and of its storage form, ABA-GE, in fruit pulp from the earliest harvest. It also increased oxidation, which was observable by the high levels of ascorbate measured at the early stages at harvest, and by the delay in the time it took to reach the pseudo constant carotene-to-xanthophyll ratio in ripe fruits. Nevertheless, differences between the irrigation treatments on the antioxidant system in ripe fruits were not significant, mainly because of the drastic changes in this system during ripening.

Role of cation structure in the phytotoxicity of ionic liquids: growth inhibition and oxidative stress in spring barley and common radish

“…The peroxidation level of protein-lipid membranes in plant organisms is commonly determined on the basis of malondialdehyde content. Thus, the MDA content is one of the most important indices, always determined in the study of oxidative stress level in plants subjected to the influence of different stress factors (Liu et al 2013 , 2014 ; Rosalie et al 2015 ; Radošević et al 2015 ). In the present paper, no considerable changes of MDA content of spring barley seedlings were observed between controls and samples cultivated on the soil with the addition of ILs.…”

Superoxide Radical Metabolism in Sweet Pepper (Capsicum annuum L.) Fruits Is Regulated by Ripening and by a NO-Enriched Environment

“…Reactive oxygen species generation is part of the cellular metabolism, and depending on the levels of production and the ROS type, they could have either toxic effects or signal properties. There are evidences that ROS metabolism is involved in a diverse range of plant processes, and the available data indicate that fruit development and ripening have an active ROS metabolism in different species such as grapevine berry (Pilati et al, 2014), mango (Rosalie et al, 2015), peach (Huan et al, 2016), tomato (Kumar et al, 2016;Zhu et al, 2017), grape berry (Xi et al, 2017), or guava (Mondal et al, 2009;Batista-Silva et al, 2018). In previous reports on sweet pepper ripening, we focused on the H 2 O 2 metabolism, and it was found that catalase activity and their gene expression were diminished during ripening.…”

Oxidative stress in spring barley and common radish exposed to quaternary ammonium salts with hexafluorophosphate anion

“…An increase in MDA content in plant cells is an indisputable index of oxidative stress and indirectly reflects the degree of cell damage. Therefore, the level of MDA is always determined when studying the extent of oxidative stress in plants subjected to different stressful biotic and abiotic factors (Liu et al, 2013;Rachoski et al, 2015;Rosalie et al, 2015).…”