Antioxidant and Cytotoxicity Activities of Karamunting (Melastoma malabathricum L.) Fruit Ethanolic Extract and Quercetin

Abstract

Melastoma malabathricum L. is a type of plant naturally grows in Kalimantan that has medicinal properties. Ethanolic extract of M. malabathricum L. flower has quercetin and kaempferol contents that have antioxidant and anticancer activities. But the antioxidant and cytotoxicity activities of M. malabathricum L. fruit ethanolic extract were not known. This study measured the quercetin and kaempferol level in M. malabathricum L. fruit ethanolic extract using HPLC MS/MS, antioxidant activity using DPPH method, and cytotoxicity activity using Brine Shrimp Lethality Test (BSLT) method. Results showed the level of quercetin and kaempferol from M. malabathricum L fruit ethanolic extract are 67.78 µg/g and 43.52 µg/g, respectively. Beside, the antioxidant activity with by IC50 was 16.82±0.24 ppm and 7.38±0.41 ppm. The cytotoxicity activities of M. malabathricum L. fruit ethanolic extract and quercetin are shown by the LC50 of 313.44 ppm (95%CI 283.97-344.43) and 37.24 ppm, respectively.

Abelmoschus esculentus subfractions attenuate beta amyloid-induced neuron apoptosis by regulating DPP-4 with improving insulin resistance signals

“…Moreover, the response to particular chemical would up to the dose apply. It was reported quercetin glycosides, which is also abundant in our F1, exhibit the antioxidation or ctotoxicity at low or high IC50, respectively [19]. Hence the dose of AE was determined and treated appropriately to attenuate the apoptosis marker in the present in vitro model (Fig 2).…”

Cytotoxic Activity of Secondary Metabolite Compounds from Myanmar Medicinal Plants

“…They also compared the extract to quercetin in terms of antioxidant and cytotoxic properties. The authors concluded that the extract exhibits antioxidant (IC 50 : 16.82 ± 0.24 ppm) and cytotoxic properties (LC 50 : 313.44 ppm) which may be attributed to the presence of quercetin [69]. The compounds such as flavonoids, naringenin, and kaempferol-3-O-(2′′,6′′-di-Op-trans-coumaroyl)glucoside, isolated from the flower of M. malabathricum, have been proved to be the potent inhibitor of MCF7 with IC 50 values of 0.28 μM and 1.3 μM [70].…”

Exploration of the Potential of Local Plants of Melastoma malabatchricum Fruit for Food Fortification

“…Fruits provide vitamins and minerals for daily diet and are a major source of phytoestrogens, anti-inflammatory agents, and antioxidants [17]. One type of herb whose fruit contains antioxidants is Melastoma malabatchricum fruit (M. malabathricum Merr) [18]; [19]. M. malabatchricum (M. mabathricum Merr) is a quite abundant shrub in Central Kalimantan.…”

“…Several research results state that certain parts of the M. malabatchricum plant have several benefits, including leaf extract as an antifungal and anti-microbial [21]. Other studies have revealed some of the benefits of M. malabatchricum fruit as a natural textile dye, natural food coloring, and antioxidant effects [18]; [19].…”