Antioxidant and Antigenotoxic Potential of Infundibulicybe geotropa Mushroom Collected from Northwestern Turkey

Abstract

Infundibulicybe geotropa (Bull.) Harmaja is an edible mushroom found in Bolu province in northwestern Turkey. The chemical composition and bioactivity of these mushrooms has not been previously investigated. We examined the phenolic composition, elemental content, and antioxidant and antigenotoxic effects of methanol extracts of fruiting bodies. The phenolic compounds in the fungal samples were determined using high-performance liquid chromatography (HPLC), and element content was determined using atomic absorption spectrophotometry. Total antioxidant status (TAS), total oxidant status (TOS), and oxidative stress index (OSI) were determined using the commercially available Rel assay kit. The antigenotoxic effects of the extract were determined using the MTT assay to assess cell viability and the alkaline single-cell gel electrophoresis assay (Comet assay). The total phenolic content (ppm) of I. geotropa was found to be catechin (361±2.31), clorogenic acid (553.54±5.06), and coumaric acid (9.93±0.25). The TAS, TOS, and OSI of the extract were 1.854±0.051 mmol/L, 30.385±0.399 μmol/L, and 1.639±0.067, respectively. The elemental levels were within “normal” range. In HT22 mouse hippocampal neuronal cells, the extract (100 and 200 μg/ml) showed no genotoxic potential and ameliorated hydrogen peroxide- (H2O2-) induced oxidative DNA damage. I. geotropa may be considered a good nutrient due to its phenolic constituents and antioxidant potential.

Antioxidant, antimicrobial and neuroprotective effects of Octaviania asterosperma in vitro

“…As shown in Table 2, the TAS value of the mushroom ethanol extract was 3.410 ± 0.099 mmol/L. The TAS value reported in this study is much higher than the TAS value reported in other mushroom extracts: Fomitopsis pinicola, Laetiporus sulphureus, Infundibulicybe geotropa and Cerioporus varius was 1.44, 2.195, 1.854 and 2.312 mmol/L respectively (Sevindik et al 2017(Sevindik et al , 2018(Sevindik et al , 2020Sevindik 2019). This may be due to the greater phenolic content of the mushroom extract under investigation.…”

“…The TOS value of O. asterosperma ethanol extract was 7.548 ± 0.147 μmol/L. This was much lower compared to the TOS value reported for F. pinicola, I. geotropa and C. varius (14.21, 30.38 and14.36, respectively), but higher than L. sulphureus , (1.303 μmol/L) (Sevindik et al 2017 , 2018 , 2020 ; Sevindik 2019 ). Differences between mushroom TOS values may be attributed to the different methodologies used by different research groups at different times and the different species of mushroom collected in different locations.…”

“…The OSI value for O. asterosperma mushroom reported in this study was 0.221 ± 0.005. On the other hand, the OSI was 0.99, 0.059, 1.639 and 0.627 for F. pinicola, L. sulphureus, I. geotropa and C. varius respectively (Sevindik et al 2017(Sevindik et al , 2018(Sevindik et al , 2020Sevindik 2019). The OSI for O. asterosperma was lower than that of F. pinicola, I. geotropa and C. varius mushrooms and higher than L. sulphureus.…”

Phenolic Content, Antioxidant and Antimicrobial Potential of Endemic Ferulago platycarpa

“…They are one of the oldest living things on the planet that existed on Earth for millions of years. Recently, the resistance of bacteria to synthetic antibiotics has become a global problem [47]. For this reason, researchers have considered the use of natural products such as medicinal plants for the production of new antibiotic drugs [48].…”

Antioxidant and Antimicrobial activity of Scorzonera papposa collected from Iraq and Turkey

“…The discovery of new antimicrobial agents from plants has been increasing in recent years. Especially the insufficiency of synthetic drugs directed the researchers to natural materials (Sevindik et al, 2020). The discovery of new antimicrobial sources has become imperative due to increased microbial resistance and increasing diseases of microorganism origin.…”