Antioxidant and Anticancer Aporphine Alkaloids from the Leaves of Nelumbo nucifera Gaertn. cv. Rosa-plena

Abstract

Fifteen compounds were extracted and purified from the leaves of Nelumbo nucifera Gaertn. cv. Rosa-plena. These compounds include liriodenine (1), lysicamine (2), (-)-anonaine (3), (-)-asimilobine (4), (-)-caaverine (5), (-)-N-methylasimilobine (6), (-)-nuciferine (7), (-)-nornuciferine (8), (-)-roemerine (9), 7-hydroxydehydronuciferine (10) cepharadione B (11), β-sitostenone (12), stigmasta-4,22-dien-3-one (13) and two chlorophylls: pheophytin-a (14) and aristophyll-C (15). The anti-oxidation activity of the compounds was examined by antiradical scavenging, metal chelating and ferric reducing power assays. The results have shown that these compounds have antioxidative activity. The study has also examined the antiproliferation activity of the isolated compounds against human melanoma, prostate and gastric cancer cells. The results shown that 7-hydroxydehydronuciferine (10) significantly inhibited the proliferation of melanoma, prostate and gastric cancer cells. Together, these findings suggest that leaves of Nelumbo nucifera Gaertn. cv. Rosa-plena are a good resource for obtaining the biologically active substances with antioxidant properties.

Identification of the anti-tumor activity and mechanisms of nuciferine through a network pharmacology approach

“…As a pilot study, the anti-tumor activities and molecular mechanisms of nuciferine, an aporphine alkaloid extracted from lotus leaves, were selected for further study and validation in our investigation, on the basis of evidence from the literature and in vivo/in vitro experiments. For example, nuciferine has been reported in studies of melanoma [16] , non-small cell lung cancer [17] and the DU-145 cell line [18] . In addition, we predicted that nuciferine and melphalan would exhibit similar anti-tumor profiles against the tested tumor cell lines.…”

Anticancer activities of alkaloids extracted from the Ba lotus seed in human nasopharyngeal carcinoma CNE-1 cells

“…cv. Rosa-plena exhibited antioxidant and anticancer activity in vitro (30). In the present study, BLSA exhibited anti-cancer effects, associated with the induction of apoptosis, in CNE-1 cells.…”

Edible freshwater macrophytes: a source of anticancer and antioxidative natural products—a mini-review

“…Sequential fractionation of active extracts with organic solvents, for example, petroleum ether, acetone, dichloromethane, ethyl acetate, butanol and methanol (Khlifi et al 2013;Tagne et al 2014) has also been reported. The cytotoxicity assay most widely used for estimating the anticancer potential of EFM is the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay (Babykutty et al 2008;Fan et al 2014;Liu et al 2014;Park et al 2005), but other assays such as the sulphorhodamine B (SRB), neutral red, and lactate dehydrogenase (LDH) leakage assays have also been used (Aboul-Enein et al 2014;Prasad et al 2005;Rose et al 2005). The MTT assay gives an indication of whole cell cytotoxicity.…”