Antioxidant and Anti-Inflammatory Activities of Hydrolysates and Peptide Fractions Obtained by Enzymatic Hydrolysis of Selected Heat-Treated Edible Insects

Abstract

This study investigated the effect of heat treatment of edible insects on antioxidant and anti-inflammatory activities of peptides obtained by in vitro gastrointestinal digestion and absorption process thereof. The antioxidant potential of edible insect hydrolysates was determined as free radical-scavenging activity, ion chelating activity, and reducing power, whereas the anti-inflammatory activity was expressed as lipoxygenase and cyclooxygenase-2 inhibitory activity. The highest antiradical activity against DPPH• (2,2-diphenyl-1-picrylhydrazyl radical) was noted for a peptide fraction from baked cricket Gryllodes sigillatus hydrolysate (IC50 value 10.9 µg/mL) and that against ABTS•+ (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) radical) was the highest for raw mealworm Tenebrio molitor hydrolysate (inhibitory concentration (IC50 value) 5.3 µg/mL). The peptides obtained from boiled locust Schistocerca gregaria hydrolysate showed the highest Fe2+ chelation ability (IC50 value 2.57 µg/mL); furthermore, the highest reducing power was observed for raw G. sigillatus hydrolysate (0.771). The peptide fraction from a protein preparation from the locust S. gregaria exhibited the most significant lipoxygenase and cyclooxygenase-2 inhibitory activity (IC50 value 3.13 µg/mL and 5.05 µg/mL, respectively).

Edible insects as a food source: a review

“…The active constituents extracted from it, including polysaccharides, flavones and beauvericin have been developed into modern medicine targeting corresponding diseases (Hu et al 2017;Wu et al 2015). The medical potential of many other reared insects have been demonstrated, including several cases of antioxidants extracted from different groups of insects (Atkinson et al 1973;Feng and Chen 2009;Zielinska et al 2017). Benefitseconomically and environmentally…”

Antioxidant Peptides from Goat Milk Fermented by Lactobacillus casei L61: Preparation, Optimization, and Stability Evaluation in Simulated Gastrointestinal Fluid

“…Free radicals are generated inevitably as by-products during aerobic metabolism, generally as reactive oxidant species (ROS) [ 1 ]. The most common ROS in vivo are superoxide, hydroxyl radical, peroxyl radical, nitric oxide, and peroxynitrite [ 2 ].…”

Comparative study of composition, antioxidant and antimicrobial activity of two adult edible insects from Tenebrionidae family

“…Specimens from the Tenebrionidae family: Adult beetle T. molitor (Linnaeus, Coleoptera: Tenebrionidae) and adult beetle U. dermestoides were kindly donated from the identified taxonomic reservoir by Dr. J. Luis Navarrete Hereida and the offspring was deposited in a plastic container where the temperature was maintained between 25 and 30 °C, once at the adult stage, insects were separated into different deposits. Food consisted of a mixture of oats and thin slices of apple (approximately 3.2-4.8 mm thick) every third day as source of hydration [11]. Insects were fasted for approximately 48 h to clear their gastrointestinal tract of any residual food before they were collected.…”

“…Authors like Nowak et al [32] point out that existing information on the contribution of insects to nutrient intake is still too scarce. While, in some extent family Tenebrionidae has been characterized in terms of nutritional value [6,7], protein production [8], cytotoxic and genotoxic activity [9,10], and some nutraceutical properties [11][12][13]. Aforementioned studies are focused only in the larvae's stage of the insect.…”