Antioxidant Activity of Syringic Acid Prevents Oxidative Stress in l-arginine–Induced Acute Pancreatitis: An Experimental Study on Rats

Abstract

The aim of this study was to investigate the possible protective role of antioxidant treatment with syringic acid (SA) on L-arginine-induced acute pancreatitis (AP) using biochemical and histopathologic approaches. A total of 30 rats were divided into 3 groups. The control group received normal saline intraperitoneally. The AP group was induced by 3.2 g/kg body weight L-arginine intraperitoneally, administered twice with an interval of 1 hour between administrations. The AP plus SA group, after having AP induced by 3.2 g/kg body weight L-arginine, was given SA (50 mg kg À1 ) in 2 parts within 24 hours. The rats were killed, and pancreatic tissue was removed and used in biochemical and histopathologic examinations. Compared with the control group, the mean pancreatic tissue total oxidant status level, oxidative stress index, and lipid hydroperoxide levels were significantly increased in the AP group, being 30.97 6 7.13 (P , 0.05), 1.76 6 0.34 (P , 0.0001), and 19.18 6 4.91 (P , 0.01), respectively. However, mean total antioxidant status and sulfhydryl group levels were significantly decreased in the AP group compared with the control group, being 1.765 6 0.21 (P , 0.0001) and 0.21 6 ), peroxynitrite, and other nitrates, whereas carbon-centered molecules are rather complex in terms of their chemical structure and generally are produced in the xenobiotic metabolism.2 Normally, there is a delicate balance between ROS and RNS production and tissue concentrations of antioxidants in the body. This balance is related to the rate of total antioxidant status (TAS) to total oxidant status (TOS), as determined by the oxidative stress index (OSI). ROS are produced both normally through the electron transfer chain system of the mitochondria and in excessive numbers in various conditions that increase energy (ATP) demand. The latter may include, among other factors, biologic factors and exposure to heat and certain chemicals and toxins. [3][4][5] ROS plays an important role in the pathogenesis of AP, and there is also a correlation between the production of ROS and the severity of AP. The detrimental effects of ROS and RNS are mediated by their direct actions on biomolecules, such as lipids, proteins, and DNA, and the activation of proinflammatory signal cascades, which subsequently lead to the activation of immune responses. 2The dietary plant polyphenolic compounds were shown to have beneficial effects in preventing oxidative stress, inhibiting the production of free radicals and the formation of lipid peroxidation.Scientific interest in phenolic compounds has been stimulated because of their anti-inflammatory, antimutagenic, and anticarcinogenic properties. They have antioxidant activity mainly due to their redox properties, which allow them to act as reducing agents, hydrogen donors, free radical scavengers, metal chelators, and modulators of enzymatic activity, thereby preventing a lot of diseases, including diabetes mellitus, hypertension, atherosclerosis, and cancer. 6,7 Antioxidants are compounds that, even when presen...

Phenolic Contents, Oxidant/Antioxidant Potential and Heavy Metal Levels in Cyclocybe cylindracea

“…27 Syringic acid is known to exhibit multiple bioactive properties such as strong antioxidant, antiproliferative, antiendotoxic, antimicrobial, anti-inflammatory and anti-cancer effects. 28 In a study, authors stated that hydroxybenzoic acid has antioxidant effect. 29 In our work, high antioxidant activity is might be due to the presence of phenolic compounds as extracted from C. cylindracea samples.…”

Effect of Syringic acid on antioxidant biomarkers and associated inflammatory markers in mice model of asthma

“…Syringic acid from the methanolic extract of Tamarix aucheriana serves as a potent inhibitor for proteasome activity and thus enhances antimitogenic activity against human breast, colorectal, and malignant melanoma cells based on molecular docking and also inhibits cell‐cycle, cell proliferation, angiogenesis, NF‐kB DNA‐binding, proteasome activity and induces apoptosis (Abaza et al, ; Orabi et al, ). A potent antioxidant activity of Syringic acid has been reported by hampering the oxidative stress markers in L‐arginine–induced acute toxicity of pancreas in rats (Cikman et al, ). Syringic acid has considerable chemopreventive effect on DMBA (7,12‐dimethylbenz[a]anthracene) induced hamster buccal pouch carcinogenesis (Periyanna, Vinothkumar, Sukumar, & Ramachandiran, ).…”

“…Antioxidants are known to modulate inflammation and attenuate oxidative stress related conditions (Cikman et al, 2015;Gao et al, 2018). Syringic acid (4-Hydroxy-3,5-dimethoxybenzoic acid) is a naturally occurring phenolic compound mainly found in fruits of edible plants and mushrooms (Itoh et al, 2009;Pacheco-Palencia, Mertens-Talcott, & Talcott, 2008).…”

Increased Oxidative/Nitrosative Stress in Common Metabolic Diseases in Gaziantep Region

“…Researchers have indicated that ROS/RNS formation processes are intimately linked to the development of the inflammatory disorders. The detrimental effects of highly reactive ROS/RNS are mediated by their direct actions on macromolecules such as lipids, proteins, and nucleic acids, and activation of proinflammatory signal cascades, which subsequently lead to activation of immune responses (Cikman, et al, 2015;Leung & Chan, 2009).…”

“…We found that oxidative/nitrosative stress was significantly increased in PKU and MSUP patients. Uncontrolled production of free radicals leads to oxidative / nitrosative damage and activation of reactive signal cascades (Cikman, et al, 2015;Leung & Chan, 2009) and therefore antioxidant treatment may be recommended as a potential treatment for disorders associated with overproduction of free radicals. Future research should focus on understanding disease mechanisms and identifying common goals to prevent or treat oxidative/nitrosative stress-induced pathologies in people with metabolic disorders.…”