Antioxidant activity of flavonoids and phenolic acids from Dodonaea angustifolia flower: HPLC profile and PASS prediction

Abstract

Background Dodonaea angustifolia is a known medicinal plant across East Arica. The flower of D. angustifolia is not well investigated in terms of phytochemistry and biological activities. This study aims to investigate the presence of flavonoids and phenolic acid in the flower of D. angustifolia and its antioxidant activity. Methods Preliminary phytochemical screening was carried out using standard protocols. Antioxidant activity evaluation using DPPH assay and total phenol content (TPC) and total flavonoid content (TFC) determinations in the flower extract was compared with the values of the leaf extract. UHPLC–DAD analysis was managed to develop the profile of the flower extract. Prediction of biological activity spectra for substances (PASS) was done using an online server for antioxidant and related activities. Results Preliminary phytochemical screening, TPC, and TFC values confirmed the presence of flavonoids and phenolic acids. From HPLC analysis of flavonoids: quercetin, myricetin, rutin, and phenolic acids: chlorogenic acid, gallic acid, and syringic acid were detected and quantified. The biological activity spectrum was predicted for the detected and quantified polyphenols. Conclusions D. angustifolia flower is a rich source of flavonoids and phenolic acids, which are extractable and can be checked for further biological activity. It was possible to identify and quantify phenolic compounds through HPLC analysis in the methanol extract of D. angustifolia flower. PASS biological activity prediction results showed that there were stronger antioxidant activities for the identified flavonoids. Future work will emphasize the isolation and characterization of active principles responsible for bioactivity.

Targeted HPTLC Profile, Quantification of Flavonoids and Phenolic Acids, and Antimicrobial Activity of Dodonaea angustifolia (L.f.) Leaves and Flowers

“…In our previous study, using HPLC analysis, we identified and quantified the three phenolic acids, including chlorogenic acid, gallic acid, and syringic acid, in methanol extract of D. angustifolia flower extract, which is in close agreement with the current HPTLC analysis result [ 34 ]. Similar to the HPLC data presented in our earlier work, the HPTLC profiles ( Figure 2 and Figure 3 ) and peak tables ( supplementary file, Tables S1 and S2 ) provide information regarding the presence of flavonoids and phenolic acids.…”

“…From a prior investigation on the flower of D. angustifolia [ 34 ], it was revealed that almost similar phytochemical screening results show both the leaf and flower are rich in phenolic acids and flavonoids. Furthermore, we have compared both samples’ antioxidant activity and total phenolic and flavonoid contents.…”

“…Similar to the HPLC data presented in our earlier work, the HPTLC profiles ( Figure 2 and Figure 3 ) and peak tables ( supplementary file, Tables S1 and S2 ) provide information regarding the presence of flavonoids and phenolic acids. From the preliminary phytochemical screening test result [ 34 ], the leaves and flowers of D. angustifolia were nearly similar in terms of phytochemical groups (phenolic acids and flavonoids) considered in this investigation. The screening investigation revealed that the main components of the D. angustifolia leaf and flower extract included flavonoids and phenolic acids [ 41 ].…”

“…Because of their inherent structural diversity for health and dietary significance, quick and accurate assessment of phenolic acids and flavonoids is crucial [ 33 ]. Our previous investigation reported that both the leaves and flower methanol extracts of D. angustifolia exhibit significant antioxidant activity [ 34 ]. Phenolic acids play a crucial role in controlling plant development and disease resistance.…”

“…Flavonoids shield plant tissue from UV exposure because of their powerful UV radiation absorption. Pharmacological studies have shown that flavonoids have antiviral, anti-allergic, anticancer, antibacterial, antifungal, and antithrombotic properties [ 33 , 34 , 36 ]. They affect blood arteries, specifically flavanones and catechins, which boost capillary resistance.…”