Antioxidant Activity of Dominant Plants Species in Obat Pahit from Lingga Malay Ethnic in Riau Archipelago

Abstract

Obat Pahit is a potion that has been long commonly consumed by Lingga Malay society for generations as stamina keeper. The most dominant plants found in the packaging of the Obat Pahit were namely Bauhunia semibifida, Cnestis palala and Penawa Root (3 species). This research aimed to investigate and determine activity of antioxidant contents in Obat Pahit from five Traditional Medicine Practitioners (TMPs) in the district of Lingga. The tested samples were mashed then being soaked into 2 types of solvent: distilled water and methanol, containing HCl 1%. DPPH method was also used in this research. Quantitatively antioxidant activity test of Obat Pahit from the five TMPs by using methanol solvent had extremely highest activity compared to the distilled water solvent. The test, using TLC plate by spraying the extract from three dominant plants with 0.1 mM of DPPH solution, produced a pale-yellow spots at a wavelength of 366 nm. On the other hand, the test using HPLC at wavelengths of 230 nm and 280 nm showed the presence of two dominant secondary metabolites contents: flavonoid and phenolic. IC50 (ppm) of Bauhinia semibifida (6.6247), Penawa Root (5.0124) and Cnestis palala (5.9968) were much lower than IC50 of mangosteen's rind (41.7675), vitamin C (6.6612) and Stimuno drug (8.333). This antioxidant analysis has not been reported previously. This proof contributed greatly to uncovering potentially native natural resources as an indigenous Indonesian drug which is expected to decrease dependence on imported drugs especially imunomodulator, antihypertensive, antidiabet etc. This research would be beneficial and excellent manifestation for the development of natural antioxidant-based medicines from traditional knowledge of Indonesia's local ethnicities.

The Potential of Liquid Tofu Waste in Increasing Antioxidant Activity of Robusta Green Coffee

“…Robusta coffee with decaffeination in 90% waste concentration for 9 hours has the highest antioxidant activity of 17,6061. A natural ingredient is said to contain very active antioxidants, if it has an IC50 of less than 50 ppm (Budryn et al, 2017;Fitmawati et al, 2017;Gebeyehu & Bikilla, 2015;Iswari & Susanti, 2016).…”

Isolasi Senyawa Metabolit Sekunder dari Ekstrak Metanol Kulit Batang Bauhinia semibifida Roxb. dan Uji Aktivitas Antioksidan

“…atau dikenal dengan kangkang katup atau pohon kupu-kupu merupakan salah satu komposisi ramuan tradisional "obat pahit" oleh masyarakat Melayu Lingga. Tumbuhan tersebut memiliki khasiat menjaga stamina tubuh, mengobati pegal linu (Lovadi and Linda, 2015) dan ramuan tersebut memiliki aktivitas antioksidan dengan nilai IC50 sebesar 6,62 ppm (Fitmawati et al, 2017).…”

Isolasi dan Uji Aktivitas Antioksidan Metabolit Sekunder dari Ekstrak Etil Asetat Kulit Batang Kangkang Katup (Bauhinia semibifida Roxb)

“…Tumbuhan Bauhinia semibifida Roxb ini oleh masyarakat Melayu Lingga dikenal dengan Kangkang katup atau dikenal juga dengan pohon bunga kupu-kupu. Dimana masyarakat Melayu Lingga tersebut menggunakan tumbuhan tersebut untuk menjaga stamina tubuh (Fitmawati et al, 2017).…”

“…Berdasarkan studi literatur menunjukkan bahwa tumbuhan Kangkang katup ini belum begitu banyak dilakukan (Fitmawati et al, 2017). Beberapa penelitian sebelumnya, Fadhli et al, (2019) telah melaporkan bahwa ekstrak etil asetat Bauhinia semibifida Roxb memiliki aktivitas antioksidan kuat dengan IC50 sebesar 30,15 µg/mL dan nilai AAI sebesasar 1.32, Selain itu, pada penelitian Tanjung & Tjahjandarie (2014) Al-Kimia |Volume 8 Nomor 1 2020 37 dengan nilai IC50 sebesar 6,6247 ppm.…”