Antiobesity effect of
            <i>Brassica juncea</i>
            cultivated in Jeongseon with optimized sinigrin content using 3T3‐L1 adipocytes

Kwon, Hee-Yeon; Choi, Sun‐Il; Han, Xionggao; Men, Xiao; Jang, Gill‐Woong; Choi, Yong Hwan; Lee, Ok‐Hwan

doi:10.1111/jfbc.13650

Cited by 6 publications

(6 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…PPAR-γ, C/EBP-α, and ap2 are crucial factors in the transformation of preadipocytes into fully developed adipocytes [ 37 ]. In a previous study, BJE was reported to inhibit lipid accumulation by downregulating protein levels of PPAR-γ, C/EBP-α, and ap2, aligning with the findings of the current study [ 30 ]. ACC regulates the condensation of lipids and is integral in the overall regulation of energy metabolism [ 38 ].…”

Section: Discussionsupporting

confidence: 92%

“…Moreover, many studies have aimed to verify the efficacy of natural substances in functional foods and anti-obesity drugs, with the intention of reducing the various side effects associated with commercially available anti-obesity drugs [ 34 , 35 , 36 ]. This research was conducted to confirm the in vivo anti-obesity effect in an animal model, expanding on the results of prior in vitro investigations on the inhibition of lipid accumulation by BJE with an optimized sinigrin content [ 30 ].…”

Section: Discussionmentioning

confidence: 99%

“…Sinigrin from Brassica juncea has been documented to exhibit various beneficial properties such as anticancer, anti-inflammatory, antibacterial, antifungal, and antioxidant effects. In our previous studies, BJE extracted under optimized sinigrin extraction conditions was reported to have anti-obesity effects in vitro [ 28 , 29 , 30 ]. Therefore, we prepared BJE using an optimized extraction method referring to previous studies and analyzed the content of sinigrin, the main active ingredient of Brassica juncea , using HPLC.…”

Section: Discussionmentioning

confidence: 99%

“…In prior research, we established a uniform extraction technique by conducting methodical experiments that focused on variables such as extraction duration, type of solvent used, and extraction temperature. Briefly, we adopted a standardized experimental method using 60% ethanol at 70 °C as the extraction solvent, and the extraction time was 3 h [ 30 ]. The materials utilized in the experiment were subjected to a washing procedure to eliminate any unwanted components, followed by freeze-drying using a freeze dryer (Ilshin, Seoul, Republic of Korea).…”

Section: Methodsmentioning

confidence: 99%

“…Sinigrin has been reported to demonstrate anticancer, anti-inflammatory, antibacterial, antifungal, and antioxidant effects. Our previous study reported that Brassica juncea extract (BJE), extracted under optimized sinigrin extraction conditions, has anti-obesity effects in vitro [ 28 , 29 , 30 ]. Furthermore, there have been reports indicating that BJE exhibits anti-obesity properties in living organisms; however, research on the specific underlying mechanism remains limited [ 31 ].…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

The Mechanism of the Anti-Obesity Effects of a Standardized Brassica juncea Extract in 3T3-L1 Preadipocytes and High-Fat Diet-Induced Obese C57BL/6J Mice

Lim,

Im,

Han

et al. 2024

Nutrients

Self Cite

View full text Add to dashboard Cite

Obesity is a global health concern. Recent research has suggested that the development of anti-obesity ingredients and functional foods should focus on natural products without side effects. We examined the effectiveness and underlying mechanisms of Brassica juncea extract (BJE) in combating obesity via experiments conducted in both in vitro and in vivo obesity models. In in vitro experiments conducted in a controlled environment, the application of BJE demonstrated the ability to suppress the accumulation of lipids induced by MDI in 3T3-L1 adipocytes. Additionally, it downregulated adipogenic-related proteins peroxisome proliferator-activated receptor-γ (PPAR-γ), CCAAT/enhancer-binding protein-α (C/EBP-α), adipocyte protein 2 (aP2), and lipid synthesis-related protein acetyl-CoA carboxylase (ACC). It also upregulated the heat generation protein peroxisome proliferator-activated receptor gamma coactivator-1α (PGC-1α) and fatty acid oxidation protein carnitine palmitoyltransferase-1 (CPT-1). The oral administration of BJE decreased body weight, alleviated liver damage, and inhibited the accumulation of lipids in mice with diet-induced obesity resulting from a high-fat diet. The inhibition of lipid accumulation by BJE in vivo was associated with a decreased expression of adipogenic and lipid synthesis proteins and an increased expression of heat generation and fatty acid oxidation proteins. BJE administration improved obesity by decreasing adipogenesis and activating heat generation and fatty acid oxidation in 3T3-L1 cells and in HFD-induced obese C57BL/6J mice. These results suggest that BJE shows potential as a natural method for preventing metabolic diseases associated with obesity.

show abstract

Section: Discussionsupporting

confidence: 92%